Matthew Kirkcaldie <m.kirkcaldie at removethis.unsw.edu.au> wrote:
> Surely you'd have to find a way to sterically hinder the action of
> whatever is desensitising the receptors? What's desensitising them?
I would like to be able to expose my slices to a prolonged GABA puff
without desensitizing the receptors.
> I can tell you that allopregnanolone is a potent endogenous potentiator
> of the GABA-A receptor, but it would be a pain to deliver in a
> controlled way - it's a steroid so you usually have to use very sticky
> vehicle.
That doesn't sound to nice. Sounds like it would stick to the perfusion
system as well.
I've been giving the idea of potentiating the currents with barbiturates
a bit of though, but don't quite not what to expect from a kinetic point
of view (I know: a look into Hille's text book will change that -
tomorrow).
Have a nice Sunday,
Christian W.
