Matthew Kirkcaldie <m.kirkcaldie at removethis.unsw.edu.au> wrote:
> Surely you'd have to find a way to sterically hinder the action of
> whatever is desensitising the receptors? What's desensitising them?
I would like to be able to expose my slices to a prolonged GABA puff
without desensitizing the receptors.
> I can tell you that allopregnanolone is a potent endogenous potentiator
> of the GABA-A receptor, but it would be a pain to deliver in a
> controlled way - it's a steroid so you usually have to use very sticky
That doesn't sound to nice. Sounds like it would stick to the perfusion
system as well.
I've been giving the idea of potentiating the currents with barbiturates
a bit of though, but don't quite not what to expect from a kinetic point
of view (I know: a look into Hille's text book will change that -
Have a nice Sunday,