Hi, I happened to pick up this message from you while looking for something
else. I double label rat 1-Gaba with gold 2-fibers dab
biocytin. There are several tricks that I employ to get these to work. I
try to keep the fiber staining light to be able to see the synapses. I
cryo protect the sections and freeze thaw the sections packed in baby
powder to improve the Gaba staining and preserve the ultrastructure. Yes,
baby powder. It works! I now enhance the gold with silver. I can give
you more details if you are interested.
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