[Neuroscience] Biocytin and double-labelling in EM

Linda Hassinger lhassinger at mclean.harvard.edu
Fri Mar 3 12:40:41 EST 2006

Hi, I happened to pick up this message from you while looking for something 
else.  I double label  rat  1-Gaba  with gold  2-fibers dab 
biocytin.  There are several tricks that I employ to get these to work.  I 
try to keep the fiber staining light to be able to see the synapses.  I 
cryo protect the sections and freeze thaw  the sections packed in baby 
powder to improve the Gaba staining and preserve the ultrastructure. Yes, 
baby powder.  It works!  I now enhance the gold with silver.  I can give 
you more details if you are interested.
Linda Hassinger

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