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[Neuroscience] Re: More questions on Cerebellar slices

Bill via neur-sci%40net.bio.net (by connelly.bill from gmail.com)
Mon Apr 9 16:57:12 EST 2007


On Apr 7, 2:47 am, usene... from out-of-phase.de (Christian Wilms) wrote:
> We use a variation of what Matthew suggested, as we are interested
> _only_ in removing the cerebellum. We also insert the scissors into the
> foramen magnum, but we cut around the cerebellum: we start the cut
> heading rostral but as soon as we clear the cerebellum we start cutting
> upwards towards the midline. Once reaching or slightly crossing the
> midline we place the same cut for the other side, freeing the part of
> the skull covering the cerebellum. Now one can use a forceps to remove
> this part of the skull - if you grab the skull at the point where your
> two cuts met and pull it off "tailwards" or grab hold of it at the
> foramen magnum and pull the skull of in an upward-rostral direction (my
> personal preference when working with rats) is a matter of taste.
>
> Using this procedure you will normally destroy large parts of the
> Cortex, so if you would want to prepare slices from several regions you
> would need to vary it - probably Matthew's approach is the best for
> that.
>
> Sorry, if I'm not making myself clear enough - I'll see if I can get a
> picture series of a prep if I've totally lost you here.
>
> Cheers, Christian

Excellent, I think I completely understand, thanks for the tip.



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