Seems reasonable to me. The procedure that is commonly used around
here is were 1.4-1.9g/kg urethane with additional ketamine at 10 mg/
kg, I.M and xylazine 2 mg/kg, I.M.and then an injection of urethane
(0.2 gm/kg) every 1-2 hr.
Maybe you should just avoid the isoflurane?
On May 1, 1:44 am, <Ana.Cervera-Fe... from uv.es> wrote:
>> I am a PhD student and I am starting with the electrophysiology. We are
> trying to record neuronal activity in rats during urethane anesthesia
> but we are having lots of problems with this. I am first anesthetizing
> with isofluorane and then 15% urethane in distilled water I.P. at 1.5 to
> 1.8 g/kg. Usually it takes a lot of time for them to loss reflexes and
> sometimes they die before we could start recording (and also they use to
> die very early). I also use local topic lidocaine in pressure points.
>> Any advise? What dose should I use?