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[Neuroscience] Re: Curing slygard?

Bill via neur-sci%40net.bio.net (by connelly.bill from gmail.com)
Mon Apr 30 20:51:18 EST 2007


Yes, it doesn't make much difference to the peak to peak noise in my
hands either. However, I was using it more for minimizing fast
capacitative artifacts; under a water immersion lens, the axopatch 200
couldn't correct for all the capacitance.

On Apr 30, 3:12 am, fbur... from nyx.net (Francis Burton) wrote:
> In article <1176412432.715383.47... from w1g2000hsg.googlegroups.com>,
>
> Bill <connelly.b... from gmail.com> wrote:
> >This may be a stupid question, but I've only ever figured out how to
> >do these things by myself, is there a better way to cure slygard on
> >your pipettes than holding it a foot above the bunsen burner? I don't
> >want to leave it for the 48 hours that it takes for the sylgard to
> >cure at room temp, (according to the dow corning website), I've bought
> >into the dogma that old patch pipettes don't work that well.
>
> When I was patch-clamping in the mid 80s, I found that Sylgaard
> made NO difference to noise levels. Moreover, the presence of a
> hydrophobic coating created a more curved meniscus which made
> manipulating the pipette in relation to the cell harder. So I
> omitted the coating step. I was studying single channel currents
> in inside-out patches from cardiac and skeletal muscle membrane,
> using an EPC-7 amplifier.
>
> YMMV, of course - but have you tried NOT Sylgarding?
>
> Francis




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