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[Neuroscience] How to evaluate cytoxicity of viral vectors

Peter Shiromani via neur-sci%40net.bio.net (by petershiromani At yahoo.com)
Wed Jan 17 12:49:15 EST 2007


I am trying to determine an assay procedure that will evaluate cytoxicity of viral vectors.  Would the following protocol work:

Electroporate or transfect PC12 cells (or suggest better cell lines such as BHK cell) with the viral vector and then at various times after the transfection measure  cytokines such as TNF-alpha etc using standard kits from commercial sources, say R&D systems or Genzyme.
Controls will be mock infections.


Will this work??

 
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