[Neuroscience] Re: Biocytin labeling in adult brain slices

Bill via neur-sci%40net.bio.net (by connelly.bill from gmail.com)
Wed Mar 14 21:00:29 EST 2007

On Mar 15, 5:25 am, "Karthik Rajasekaran" <k... from virginia.edu> wrote:
> Hi
> I have only limited success (approx. 50%) with biocytin labelling of neurons
> from which I record whole cell currents from thalamic relay neurons. The
> protocol that I use is fairly standard in which singal is amplified by
> incubating the sections in ABC and then reacted with DAB. The slices are
> obtained 8-9 month old rats, and often those that present with extensive
> gliosis....though I do not see any reason why that would affect it as long
> as I get my cell to record...  Any advice / suggestions will be greatly
> appreciated. Thank you!
> Karthik

How are you pulling your electrode off the cells? Fast, slow?

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