On Mar 15, 5:25 am, "Karthik Rajasekaran" <k... from virginia.edu> wrote:
> I have only limited success (approx. 50%) with biocytin labelling of neurons
> from which I record whole cell currents from thalamic relay neurons. The
> protocol that I use is fairly standard in which singal is amplified by
> incubating the sections in ABC and then reacted with DAB. The slices are
> obtained 8-9 month old rats, and often those that present with extensive
> gliosis....though I do not see any reason why that would affect it as long
> as I get my cell to record... Any advice / suggestions will be greatly
> appreciated. Thank you!
How are you pulling your electrode off the cells? Fast, slow?