[Neuroscience] cold PFA

Kimberly Robin Thompson via neur-sci%40net.bio.net (by thompski from stanford.edu)
Sun Mar 18 12:48:36 EST 2007

I fixed my cultured hippocampal neurons on ice for 30 min with 4%PFA and
later found that many of the cell bodies were missing.  If I fix at room
temperature, all is fine.  Any thoughts on why temperature has such an

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