[Neuroscience] Re: Excessive DC drift during field potential recording

J.A.Legris via neur-sci%40net.bio.net (by jalegris from sympatico.ca)
Fri Nov 30 09:05:47 EST 2007

On Nov 28, 4:37 pm, Bill <connelly.b... from gmail.com> wrote:
> Hi,
> We're trying to record cortical spreading depression in rat brain
> slices, you microinject some 26mM KCl solution and it induces a 5-20mV
> spreading wave of depolarization across the cortex.
> The problem is we're getting really bad DC drift, the potential drifts
> up and down, sometimes by as much as +/-10mV.  Turning ON the
> temperature controller makes it worse (presumably because of
> temperature swings). The hight of the tissue bath doesn't seem to be
> changing.
> Any ideas on what could be causing the drift, and hence how to get rid
> of it?
> (Oh, and I can't just AC couple the data, the spreading depression
> waves are 10+s long).

Can you post a link to circuit diagram of your setup?


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