On Nov 28, 4:37 pm, Bill <connelly.b... from gmail.com> wrote:
> Hi,
>> We're trying to record cortical spreading depression in rat brain
> slices, you microinject some 26mM KCl solution and it induces a 5-20mV
> spreading wave of depolarization across the cortex.
>> The problem is we're getting really bad DC drift, the potential drifts
> up and down, sometimes by as much as +/-10mV. Turning ON the
> temperature controller makes it worse (presumably because of
> temperature swings). The hight of the tissue bath doesn't seem to be
> changing.
>> Any ideas on what could be causing the drift, and hence how to get rid
> of it?
>> (Oh, and I can't just AC couple the data, the spreading depression
> waves are 10+s long).
Can you post a link to circuit diagram of your setup?
--
Joe
