On Oct 22, 2:59 pm, Han Xu <h... from cns.nyu.edu> wrote:
> Hi All,
>> I recently study long-term synaptic plasticity in neocortex with
> patch clamp recording. The biggest problem I am facing is that series
> resistance is always going up over time, which decreases synaptic
> response amplitude. As a result, it is impossible to define the real
> potentiation or depression after conditioning stimuli. One thing I
> doubt is the internal pipette solution with an osmolarity of 271
> mOsm, which is low compared with the value mentioned in literature in
> the range of 290-310 mOsm. Is there anybody has experience on this
> problem? I will be really appreciated if somebody can give me any
> suggestions.
>> Best,
> Han
Can you explain why the series resistance (presumably of the membrane
but unclear) is increasing? Or if that is unknown, how it is being
measured?
