Thank you for the message. I have been able to solve the issue, by
fabricating a heavy harp, and also leaving quite a bit of millipore membrane
around the slice. It works fine, except that I now plate only 3-4 explants
per well. Thanks again.
"mathneuro" <mathneuro from gmail.com> wrote in message
news:1193107201.958342.15710 from v29g2000prd.googlegroups.com...
> On Oct 5, 10:31 am, "Karthik Rajasekaran" <k... from virginia.edu> wrote:
>> Dear Members
>> I am trying to perform patch clamp recordings from hippocampal slice
>> cultures in our lab, but find it tough compared to recording from acute
>> slices. Part of the problem, I think is that its difficult to securely
>> anchor down the slice, the explants are attached to the Millipore
>> membrane. When I try to reach a neuron that is slightly deeper (maybe 75
>> microM), the whole slice moves. If someone has experience with recording
>> from slice cultures, I would greatly appreciate receiving your advice on
>> what should I do (and not do) when recording from slice cultures? Thank
>> you so much.
>> Are you using a harp to hold the slice down?