Hi everyone,
I'm new on this forum and here is my first question. Do you have =
experience with TOPRO3. I commonly use it to stain DNA for confocal =
imaging and it compares well to TOTO3 or IP for example because it does =
not stain RNA and therefore you don't need to go through RNAse =
treatment. The problem is that the staining intensity of the nucleus can =
show variations from one experiment to the other. I feel that TOPRO3 =
might be rather fragile and staining efficiency might be affected after =
a few months storage of TOPRO3 at -20=B0C even if you take care to avoid =
repeted freeze/thaw. What is your experience? Also what is the best =
fixative for DNA staining (I commonly use PFA 4%)?
Thank You
Bruno=20
Bruno SAUBAMEA
EA 3621 & Service Commun d'Imagerie Cellulaire et Mol=E9culaire
Facult=E9 des Sciences Pharmaceutiques et Biologiques
Universit=E9 Paris Descartes
4, avenue de l'Observatoire
75006 PARIS
tel : 01.53.73.97.13
fax : 01.53.73.99.09
