my experiment is transplanted the neurons to the nerve tissue of host.
Now I want to observe the synapse between the transplanted neurons and
host neurons. So before transplantation, i go to find a dye to
staining the transplanted neurons.
this dye shouldnot cross the synapse,so I can obsevre the synapse in
and the experimentsin vivo lasts a long time ,I want to it keep the
fluorescence for about 4 month.
is there a dye recommended? does DiI or Dio meet the requirement? I
really donnot konw if DiI can cross the synapse.