On Dec 8, 10:12 pm, usene... from out-of-phase.de (Christian Wilms) wrote:
> Especially when switching solutions from hypoosmol to "normal ACSF" it
> is very important to slowly exchange solution. Ideally, a peristaltic
> pump will be pumping regular ACSF into the bath containing the finished
> slices while remove excess solution at the same time. A good rate should
> be 2 to 5 mL per minute.
>> If you use ascorbic acid or its salt form sodium ascorbate shouldn't
> matter, as long as you adjust the pH properly. In both cases the two
> forms will be present in an equilibrium that will depend on the pH of
> your solution (according to the Henderson-Hasselbalch equation).
>> h2h, Christian
Thanks for your answer. But I still don't understand the reason for
using hypoosmol solution. What good do hypoosmol solution?
