[Neuroscience] rat brain perfusion and fixation

[Mary P. Brownson]

Hello,

 

I am working on perfusion and fixation of rat brains for the purpose of
Fluoro-Jade staining after the brain has been sectioned with a cryostat.
I am having problems with the brain sections looking like "Swiss
cheese".  Has anyone else had this problem?  What can I do about this
problem?  I am using 4% paraformaldehyde in PBS.  Any info/hints will be
greatly appreciated.

 

Thank you,

 

Mary Brownson, Ph.D.

School of Pharmacy

University of Wyoming

(307)766-6748