Hi,
You may want to try approaching your cells with low positive pressure. Test
if they are sensitive to the pressure in your recording pipette. Cells
should stick to the poly-lys coverslips. Give them a few hours to do it so.
best,
eva
On Sat, May 10, 2008 at 9:23 AM, <anjum_zfr from yahoo.com> wrote:
> Hi ,
> we are using newborn hipppocampal neurons for patching at JCSMR, ANU,
> Canberra. they grow well and stick to glass coverslipes as well.
> Though I am new student here and learning patching here. I don't have
> the references with me rightnow but i can send you them if you wish.
>> Cheers
> Anjum
>> J.A.Legris wrote:
>> > On Apr 24, 2:15�am, christinela... from hotmail.com wrote:
> > > Hi, I'm wondering if anyone here has done electrophysiology on acutely
> > > dissociated neurons? I've found several methods in the literature and
> > > have had a couple of attempts with adult rat brain. The cells come out
> > > looking bright and round and alive, but unfortunately I can't make
> > > them stick to glass chips, with or without poly-D-lysine coating. I
> > > need them to stick to something in order to patch them.
> > >
> > > Any suggestions?
> >
> > How about a micropipette under low negative pressure.
> >
> > --
> > Joe
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--
Ewa
