How about an immerse in Silver nitrate solution (10%) for 15 min
take the slides out and wash with distilled water
and then add Ammonium Hydroxide solution (1%) to be just clear
put the slides back, stain for 30 min
then JUST wash with distilled water?
Do you think this works?
Anyone has an idea on the neurochemical bases for this reaction/
Or anyone can introduce me some quick protocol for silver staining?