Saif,
I'm not sure what 2vo refers to, but I've done quite a few surgeries on
rats. However, the best person to consult in the matter would be a
Veterinary Technician or a Veterinarian if you have access to one.
Rats should be able to push themselves up (and have control over their body
temperature) about 4-5 hours after the last dose of anesthesia. And 5-10ml
of Lactated Ringer's saline (0.9% NaCl) is also typical.
If your rats aren't waking up at all, some things to consider in your case
could be:
Anesthisia
-Are the rats receiving the correct dose of anesthesia before surgery
(should be about 40-80mg/kg Ketamine with 5-10mg/kg Xylazine)?
-And if your surgery lasts more than an hour, are they receiving the correct
maintainance doses (maintanance doses are lower, usually the inital volume
multiplied by about 0.38)?
Temperature Maintanance
-Are they kept warm (but not too warm) during the surgery?
Blood loss
-Are the rats bleeding a lot during your surgery, and is the bleeding
stopped before the end of surgery?
Hope this helps,
Mike Smeltzer
On Wed, Dec 16, 2009 at 7:56 AM, saif <saif_saad83 from hotmail.com> wrote:
> Dear sir,
> im using ketamine/xylazine for 2vo surgery in male sprogue dawely rat, I
> lose a lot of my rats post-operatively, the problem how much time dose the
> rat require to have adequate temperature(isnt it within 4-5 hours
> postoperatively), and about the fluid supplement is recommended to give all
> the rats 10 ml normal saline.
> dr.saif master researcher.
> thank you
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