That is a problem! If you weren't at such a deep location I would say
just apply high strength CNQX to the cortex (works when you're in
cerebellar granule cells). I have looked for the exact same thing in
the more mundane situation of patching in slices, and I couldn't find
However, I wonder if it would be possible to find a non-selective
sodium channel antagonist. I say this because you can apply DIDS
intracellularly to block GABAA receptors, and it is just a Cl- channel
blocker, mostly used by epithelia researchers.
What do you think you're chances are if you picospritzed the drug on?
You should be able to get a drug application electrode in their first,
and the blowing of CNQX shouldn't distrupt your patch very often (it
doesn't in slices).
Sorry I couldn't be more help.
On Feb 6, 10:01 am, Jose Guzman <n... from neurohost.org> wrote:
> Hi everybody there,
>> I have been looking in the pubmed for a long time, and I did not find
> any AMPARs blockers for intracellular application (apart from the
>> The problem is that while extracellular stimulation in vivo is quite
> straightforward, we still do not if this response is uniquely
> excitatory. Applying the drugs extracellularly is difficult due to the
> nature of the experiments (in vivo recordings).
>> Does anybody of you know something related with that?. The idea would be
> to discard the AMPAR component of a EPSC in the granule cells of the
> hippocampus upon entorinal cortex stimulation.
>> Thank you very much in advance.