I am new to the electrophysiology world and am performing hippocampal
field recordings on acute slices. I am currently getting a nice field
response, however, many times I barely see a fiber volley, (if at all)
which will sometimes decrease as I increase stimulation. Additionally,
because they (the fiber volleys) are so small, any tiny variation
radically changes the input/output slopes. My feeling is that this is
due to the large stimulation artifact. Does anybody have any ideas on
what may be causing this and how to fix it?