[Neuroscience] Fiber Volley/Stimulation artifact

bmidthun from ucsd.edu via neur-sci%40net.bio.net (by bmidthun from ucsd.edu)
Tue Jun 30 23:29:28 EST 2009


I am new to the electrophysiology world and am performing  hippocampal
field recordings on acute slices.  I am currently getting a nice field
response, however, many times I barely see a fiber volley, (if at all)
which will sometimes decrease as I increase stimulation.  Additionally,
because they (the fiber volleys) are so small, any tiny variation
radically  changes the  input/output slopes.  My feeling is that this is
due to the large stimulation artifact.  Does anybody have any ideas on
what may be causing this and how to fix it?


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