Hi,
i usually resect the meninges and then use the pressure from a 20ml
syringe to go down
once on the top of my zone of interest i use only mouth pressure
all that works well for deep brain region
if you work on superficial layers of cortex you might want to use a
different strategy
F
On 12/03/2010, at 4:51 PM, Bill wrote:
> Hi,
>> I'm learning to do In vivo patching. However, I am getting conflicting
> advice from my boss and from published methods/protocols papers.
>> Specifically, what positive pressure do you use while penetrating the
> meninges and unwanted structures, and what pressure do you use while
> searching for cells in the target area?
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------------------------------------------------------------------------
Francois Windels, PhD.
Research fellow
Synaptic plasticity laboratory
Thinking systems project (ITEE),
Queensland Brain Institute,
Saint Lucia, Australia
