Please see
> Richter, D. W., Pierrefiche, O., Lalley, P. M. and Polder, H. R.
> (1996). Voltage-clamp analysis of neurons
> within deep layers of the brain. J.Neurosci.Meth. 67, 121-131.
and also the attachment.
Best regards
HR Polder
neur-sci-request from oat.bio.indiana.edu schrieb:
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>> ------------------------------------------------------------------------
>> Today's Topics:
>> 1. Re: Anyone do in vivo patching? (Francois Windels)
>>> ------------------------------------------------------------------------
>> Betreff:
> Re: [Neuroscience] Anyone do in vivo patching?
> Von:
> Francois Windels <f.windels from uq.edu.au>
> Datum:
> Mon, 15 Mar 2010 13:21:07 +1000
> An:
> Bill <connelly.bill from gmail.com>
>> An:
> Bill <connelly.bill from gmail.com>
> CC:
>neur-sci from magpie.bio.indiana.edu>>> Hi,
> i usually resect the meninges and then use the pressure from a 20ml
> syringe to go down
> once on the top of my zone of interest i use only mouth pressure
> all that works well for deep brain region
>> if you work on superficial layers of cortex you might want to use a
> different strategy
>> F
>> On 12/03/2010, at 4:51 PM, Bill wrote:
>>> Hi,
>>>> I'm learning to do In vivo patching. However, I am getting conflicting
>> advice from my boss and from published methods/protocols papers.
>>>> Specifically, what positive pressure do you use while penetrating the
>> meninges and unwanted structures, and what pressure do you use while
>> searching for cells in the target area?
>> _______________________________________________
>> Neur-sci mailing list
>>Neur-sci from net.bio.net>>http://www.bio.net/biomail/listinfo/neur-sci>> ------------------------------------------------------------------------
> Francois Windels, PhD.
> Research fellow
> Synaptic plasticity laboratory
> Thinking systems project (ITEE),
> Queensland Brain Institute,
> Saint Lucia, Australia
>>>>>>>> ------------------------------------------------------------------------
>> _______________________________________________
> Neur-sci mailing list
>Neur-sci from net.bio.net>http://www.bio.net/biomail/listinfo/neur-sci
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