--- On Mon, 3/29/10, polly jo <jopower from yahoo.com> wrote:
> From: polly jo <jopower from yahoo.com>
> Subject: Re: [Neuroscience] western blotting\troubleshooting
> To: Neur-sci from magpie.bio.indiana.edu, "letizia polito" <letizia.polito from gmail.com>
> Date: Monday, March 29, 2010, 8:22 PM
>>> --- On Mon, 3/29/10, letizia polito <letizia.polito from gmail.com>
>> > From: letizia polito <letizia.polito from gmail.com>
> > Subject: [Neuroscience] western
> > To: Neur-sci from magpie.bio.indiana.edu> > Date: Monday, March 29, 2010, 2:24 PM
> > Hi all,
> > I have some problems with WB.
> > Frequently I obtain WB with some bands missing. ((Most
> > time i missed 3\4
> > bands on the right or on the left, but sometimes also
> > the middle) I'm
> > pretty sure that there are not bubbles since I check
> > membrane with red
> > ponceau.
> > I also thought to ECL coverage but I recently use a
> > large amount of ECL
> > for 1 membrane.
>> We could help you better if you gave more details on the
> blotting technique you use, eg., which apparatus, wet or
> semidry; which type of paper, nitrocellulose or PVDF;
> assembly of the sandwich, buffers used, time,
>> > Have you got any suggestion?
> > Thanks,
> > Letizia
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