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[Neuroscience] Cs-gluconate; Cs-methanesulfonate

becku from staff.uni-marburg.de via neur-sci%40net.bio.net (by becku from staff.uni-marburg.de)
Tue Sep 18 07:49:26 EST 2012


Dear Mrs. Dvorak,

I´m concerning to this comment:

Cs-gluconate; Cs-methanesulfonate
Hannah Dvorak hdvorak at cns.caltech.edu
Mon Mar 4 12:59:58 EST 1996

.....Here's our recipe for a Cs-gluconate-based solution for whole-cell
recording from hippocampal neurons in slice.

For 100 mL of solution:
					final concentration

3.923 g	gluconic acid (Aldrich G195-1)	100 mM
	CsOH 50% in H20 (Aldrich 23,204-1)
7.6 mg	EGTA (Sigma E4378)		0.2 mM
101.65 mg MgCl (Sigma M-8266)		5 mM
953.2 mg HEPES (Sigma H-3375)		40 mM
101.4 mg Mg-ATP	(Sigma A-9187)		2 mM
15.6 mg Li-GTP	(Sigma G-5884)		0.3 mM
34.3 mg QX-314 (optional)		1 mM

.....I haven't done much whole-cell recording myself, so I can't
vouch for the effectiveness of this solution, but it works
fine in other people's hands in our lab.

- Hannah
--
Hannah Dvorak			hdvorak at cns.caltech.edu
Division of Biology 216-76
California Institute of Technology, Pasadena, CA 91125



Can you give me information about the company and the order number  
from QX-314?

This will be great.

Kind regards
Ute Beck

Philipps-Universität Marburg
Fachbereich Medizin
Institut für Physiologische Chemie
AG Prof. Schratt
Ute Beck
Karl-von-Frisch-Str. 1
D-35043 Marburg, Germany
Tel : ++49-6421-28-62294
Fax: ++49-6421-28-66957
E-Mail: becku from staff.uni-marburg.de






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