[Neuroscience] Re: Help with series resistance and EPSP variations

Hans Reiner Polder via neur-sci%40net.bio.net (by support from npielectronic.com)
Sun Oct 23 13:31:24 EST 2016

Please see the also this paper:

Riedemann T, Polder HR, Sutor B (2016) *Determination and compensation 
of series resistances during whole-cell patch-clamp recordings using an 
active bridge circuit and the phase-sensitive technique*.
Pflügers Archiv - European Journal of Physiology, October 2016, Volume 
468, Issue 10, pp 1725–1740, http://dx.doi.org/10.1007/s00424-016-1868-8

A method which avoids any series resistance can be found in the 
following paper (sharp electrode and whole cell patch recordings):

Richter DW, Pierrefiche O, Lalley PM, Polder HR (1996) *Voltage-clamp 
analysis of neurons within deep layers of the brain.* J Neurosci 
Methods. <https://www.ncbi.nlm.nih.gov/pubmed/?term=richter+polder#> 
1996 Aug;67(2):121-3.
  PDF available under this link 

Best regards

Hans Reiner Polder
support from npielectronic.com

Am 21.10.2016 um 22:17 schrieb mathewjones from wisc.edu:
> On Friday, November 23, 2012 at 4:36:59 PM UTC-6, Jeffrey Lopez wrote:
>> Hi everybody, there is a question I have since some time but still
>> dont have a convinvcing answer.
>> When your series resistance changes during a patch clamp experiment,
>> 1- which influence does it have on my EPSP slope and amplitude?
>> 2-why?
>> 3-how would it help or not to use a discontinuous (switching) amplifier?
>>   If anyone could help me to understand I will appreciate it, please
>> try to be explicit in answering. Thanks a lot in advance!
> This page has a very nice explanation of why series resistance, pipette capacitance, and also the non-negligible effect of amplifier input impedance introduce artifacts and filtering of signals in current clamp:
> http://www.billconnelly.net

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