Chloroplast Suspension Protocol?

singsaas at singsaas at
Thu Jul 27 15:30:27 EST 1995

Ben Levin <benjinsl at> wrote:

>  I'm looking for a protocol for separating chloroplasts (centrifugation,
> yes?) 

I've had good luck with the method of Keegstra et al. (sorry, I don't have
the reference handy).  It uses a Percoll gradient and is fairly quick.
Eric L. Singsaas
UW-Madison Dept. of Botany

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