Plant proteins extraction

Denis Marc marcd at bota.ucl.ac.be
Wed Feb 2 04:16:02 EST 1994


I realize for the moment proteins extraction from flower organs grinded in
liquid N2 with a boiling SDS buffer (4% SDS, 5% mercaptoethanol, 5%
sucrose) followed by incubation in an acetone buffer for one hour a -20¡C.

So, question is : Is the acetone step necessary and if it is, what is its
interest?

Thanks in advance
D.M.



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