From ricelle from kashawaii.com Fri Sep 4 20:59:41 2009 From: ricelle from kashawaii.com (Ricelle) Date: Sat Sep 5 10:42:50 2009 Subject: [Plant-biology] Chlorella transformation vector Message-ID: Dear Sir/Madam, Our laboratory is currently planning to do Chlorella transformation but we don't have any available Chlorella transformation vectors at hand. Could you please provide us with a Chlorella transformation vector that we could use as a positive control for our future transformation experiments for research purposes only? Thank you for your kind consideration. Yours truly, Ricelle Acob Kuehnle Agrosystems, Inc Honolulu, HI 96822 From bjbliss from gmail.com Sun Sep 6 15:15:36 2009 From: bjbliss from gmail.com (Barbara Bliss) Date: Sun Sep 6 23:15:40 2009 Subject: [Plant-biology] Chlorella transformation vector In-Reply-To: References: Message-ID: This is a little disingenous, don't you think? Given that Kuehnle Agrosystems, Inc is a for-profit corporation and your "research" is intended for profit-making purposes? May I suggest you do a lit search and write to the author of an article describing the protocol you want to employ and follow the normal channels for incorporating the discoveries of others into your business plan. On Fri, Sep 4, 2009 at 9:59 PM, Ricelle wrote: > Dear Sir/Madam, > > > Our laboratory is currently planning to do Chlorella transformation but we > don't have any available Chlorella transformation vectors at hand. Could you > please provide us with a Chlorella transformation vector that we could use > as a positive control for our future transformation experiments for research > purposes only? Thank you for your kind consideration. > > > Yours truly, > > > Ricelle Acob > Kuehnle Agrosystems, Inc > Honolulu, HI 96822 > > _______________________________________________ > Plantbio mailing list > Plantbio@net.bio.net > http://www.bio.net/biomail/listinfo/plantbio > From avijitcrri from gmail.com Sun Sep 6 23:38:28 2009 From: avijitcrri from gmail.com (avijit das) Date: Mon Sep 7 15:31:03 2009 Subject: [Plant-biology] endosperm specific promoter. Message-ID: <9a849b580909062138oc0ebd0gea02a38513ee64e7@mail.gmail.com> Dear Sir/Madam, I am working on transformation of cereals. I would like to use an endosperm specific promoter. Can anybody provide me with a vector having endosperm specific promoter. Yours sincerely, Avijit -- Dr. Avijit Das, ARS Senior Scientist Division of Biochemistry Central Rice Research Institute Cuttack - 753 006, Orissa, INDIA From donslagle55 from yahoo.com Sun Sep 13 21:10:07 2009 From: donslagle55 from yahoo.com (Don Slagle) Date: Mon Sep 14 12:38:38 2009 Subject: [Plant-biology] acorn germination Message-ID: <730802.44183.qm@web111913.mail.gq1.yahoo.com> in your article, you say to soak red oak in aerated water for a couple days. should this be done before or after stratification? ? thanks don From mmyvusenet from invalid.invalid Thu Sep 17 16:00:21 2009 From: mmyvusenet from invalid.invalid (mmyvusenet) Date: Thu Sep 17 18:23:07 2009 Subject: [Plant-biology] Photos of Pelargonium Message-ID: <7hfmbsF2t76soU1@mid.individual.net> Hello: In this moment It is winter in my country but sometimes there is bright sun, I did these photos in those different moments: http://www.flickr.com/photos/mmyv/3891124494/ http://www.flickr.com/photos/mmyv/3890325791/ http://www.flickr.com/photos/mmyv/3890376191/ Thanks for your comments. -- MMYV http://www.mmyv.com From michael from beaverbell.co.uk Sat Sep 19 01:17:43 2009 From: michael from beaverbell.co.uk (Michael Bell) Date: Sun Sep 20 12:22:21 2009 Subject: [Plant-biology] Bigger alder seeds and unusual cones Message-ID: <1e1b189d50.michaelbell@michael.beaverbell.co.uk> 10 Cambridge Avenue Forest Hall Newcastle -upon - Tyne NE12 8AR michael@beaverbell.co.uk 0191 266 6435 I have already told you about my project to develop alder (Alnus) as a grain crop. But alder seeds are very small and so I want bigger ones. It is now getting to the time of year to go looking for them. I want bigger seeds. Seeds are normally hard to see in the cones, if you know of a systematic way of looking for bigger seeds I would love to know of it. But if the seeds are really big, the overlapping pattern of the scales of the cones might be distorted. I would be very grateful for anything you can find. I would like:- Cuttings. Cuttings keep the combination of genes which produced that effect. Seeds. Yes, obviously. Statement of where the tree is, Map reference GPS coordinates You take me to see the tree. I am running this project like an "Open source" computer project. My strains and the finished breed will be available to all. I will be away for a month. My nephew is marrying a Nepali girl in Kathmandu, and I am going to the wedding. I have been told to expect "heroic eating and drinking" and after that, a a walk in "the hills" - the Himalays! Enjoy the "Season of mists and mellow fruitfulness" Michael Bell -- From oclawson75 from gmail.com Mon Sep 21 21:25:43 2009 From: oclawson75 from gmail.com (Olin Clawson) Date: Tue Sep 22 08:04:42 2009 Subject: [Plant-biology] new mexico oak Message-ID: <4d8890d30909211925s457004achd10365c10f594e60@mail.gmail.com> I was searching the web for information on how to properly germinate Gambrel Oaks and I came across a few emails from you on other species. My little kids have gathered several Gambrel Acorns and are very excited to plant them this spring. Any help would be greatly appreciated. Regards Olin Clawson From bae from cs.toronto.no-uce.edu Wed Sep 23 22:51:20 2009 From: bae from cs.toronto.no-uce.edu (bae@cs.toronto.no-uce.edu) Date: Thu Sep 24 07:52:08 2009 Subject: [Plant-biology] Re: new mexico oak References: Message-ID: <2009Sep23.235120.29306@jarvis.cs.toronto.edu> In article , Olin Clawson wrote: >I was searching the web for information on how to properly germinate Gambrel >Oaks and I came across a few emails from you on other species. My little >kids have gathered several Gambrel Acorns and are very excited to plant them >this spring. Any help would be greatly appreciated. > >Regards >Olin Clawson Seeds of most temperate climate trees need to be 'vernalized', i.e. exposed to cool but not freezing temperatures for some time while they are in a moist environment before they will germinate. While the optimal conditions have been worked out for a number of species, the simplest method for a tree native to your area is to plant fresh seeds in a pot and bury the pot in your garden until spring. You may want to cover the pot with some wire screening to protect the acorns from squirrels. It wouldn't hurt to dump some dead leaves or other mulch on top of the buried pot for a little extra protection. That said, don't be too optimistic, since acorns are often infested by insect larvae which may eat the embryo. Also, seeds of wild plants don't always germinate all at once -- some may not germinate for a year or more. I don't know if oaks are like that. This sounds like a fun project for you and your kids. Think about where you want to plant the seedlings. They'll grow up with your kids, the whole experience will provide some rather nice lifelong memories for them, and some day they may be able to show their children and grandchildren the large trees that wouldn't be there but for you and them taking the time to start them from acorns now. I hope more knowledgable people will provide more and better advice for you.