From MOlney from stmartin.edu Mon Nov 17 16:55:52 2008 From: MOlney from stmartin.edu (Olney, Margaret A.) Date: Mon Nov 17 19:20:30 2008 Subject: [Plant-education] Agrobacterium? Message-ID: One of my undergraduate students would like to conduct a study looking at the transformation efficiencies of Wisconsin Fast Plants using Agrobacteria. We have not successfully found any commercial sources for Agrobacteria tumefaciens. Do any of you know of a commercial or non-commercial source? We only need a stab that we could grow and from which we could prepare our own competent cells. Thanks! Best regards, Margaret Olney Assistant Professor of Biology Saint Martin's University 5300 Pacific Ave SE Lacey, WA 98503 360-438-4327 molney@stmartin.edu From james.perry from uwc.edu Mon Nov 17 22:23:28 2008 From: james.perry from uwc.edu (Perry, James) Date: Mon Nov 17 22:36:02 2008 Subject: [Plant-education] Agrobacterium? In-Reply-To: References: Message-ID: Margaret, You may be able to get the culture from American Type Culture Collection. Alternatively, try to contact someone in Plant Pathology at a regional Land Grant R1 university and see if they are willing to provide the culture. Things have changed since my days of maintaining a culture collection and it may be that federal requirements for exchanging pathogens has made things more difficult. Another resort is to try to contact Paul Williams, Department of Plant Pathology at the University of Wisconsin-Madison. (The other UW for the left-coasters.) Paul developed WFP, so he might help. He's retired but not really I don't think. Jim Perry University of Wisconsin-Fox Valley -----Original Message----- From: plant-ed-bounces@oat.bio.indiana.edu [mailto:plant-ed-bounces@oat.bio.indiana.edu] On Behalf Of Olney, Margaret A. Sent: Monday, November 17, 2008 3:56 PM To: plant-ed@magpie.bio.indiana.edu Subject: [Plant-education] Agrobacterium? One of my undergraduate students would like to conduct a study looking at the transformation efficiencies of Wisconsin Fast Plants using Agrobacteria. We have not successfully found any commercial sources for Agrobacteria tumefaciens. Do any of you know of a commercial or non-commercial source? We only need a stab that we could grow and from which we could prepare our own competent cells. Thanks! Best regards, Margaret Olney Assistant Professor of Biology Saint Martin's University 5300 Pacific Ave SE Lacey, WA 98503 360-438-4327 molney@stmartin.edu _______________________________________________ Plant-ed mailing list Plant-ed@net.bio.net http://www.bio.net/biomail/listinfo/plant-ed