Isozyme analysis using agarose gels?

David L. Robinson dlrobi02 at HOMER.LOUISVILLE.EDU
Thu Nov 7 20:09:34 EST 1996

Does anyone out there have any experience doing Native-protein 
electrophoresis and enzyme activity staining using horizontal 
agarose gels (instead of vertical PAGE)?

I think this would be a easier, cheaper, and safer technique for 
undergraduates to do isozyme work, but am not sure of the 
feasibility. Horizontal starch gels were the norm in early isozyme 
research, and I have _heard_ of agarose being used successfully, but 
I need a reference.


Dave Robinson
Biology Dept.
Bellarmine College
Louisville, KY

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