[Plant-education] stain vascular tissue?

Jensen, Douglas via plant-ed%40net.bio.net (by Doug.Jensen At Converse.edu)
Wed Feb 7 17:55:57 EST 2007


Toluidine blue also works for lignified and suberized tissues, and it's
safer than phloroglucinol.  I have never mixed it up, so I don't know
the recipe, but I've seen references to 0.1% and 0.05%.

I still prefer phloroglucinol myself, but that may be because I like the
red color.

________________________________
Douglas P. Jensen
Chair of Biology
Converse College
Spartanburg, South Carolina, 29302
douglas.jensen At converse.edu
(864)596-9123

-----Original Message-----
From: plant-ed-bounces At oat.bio.indiana.edu
[mailto:plant-ed-bounces At oat.bio.indiana.edu] On Behalf Of David W.
Kramer
Sent: Wednesday, February 07, 2007 3:17 PM
To: plant-ed At magpie.bio.indiana.edu; sjohnson
Subject: Re: [Plant-education] stain vascular tissue?

Phloroglucinol can be used to stain lignified cells (xylem).  Make up 
a solution:

	EtOH, 95%	100 ml
	HCl (conc)	  16 ml
	Phloroglucinol	  0.1 g

Place a drop of phloroglucinol solution onto the tissue (thin 
section) and incubate 30-60 min. [temperature is not indicated!]

Result:  Lignified cell walls stain red.  Because the dye fades in a 
short time, the preparation is not permanent.

This is taken directly from page 149 of   Ruzin, Steven E.  1999.  
Plant Microtechnique and Microscopy.  New York:  Oxford University 
Press

>can anybody suggest a stain for xylem &/or phloem of  'live' plant
material?
>
>sandra
>
>
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-- 
*********************
David W. Kramer, Ph.D.
Asst. Prof. of Evolution, Ecology, and Organismal Biology
Ohio State University at Mansfield
1680 University Drive
Mansfield, OH  44906-1547
Phone:  (419) 755-4344      FAX:  (419) 755-4367
e-mail:  kramer.8 At osu.edu
http://www.mansfield.ohio-state.edu/~dkramer/
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