do detergents interfere in ELISA?

Regina Shaw regina at biotech.ufl.edu
Wed Jan 24 11:36:28 EST 1996


I am purifying a fairly unstable, hydrophobic protein (under denaturing
conditions, in gel electrophoresis). This protein will be used as an
antigen in hybridoma screening per ELISA. Is it ok to add a detergent
(e.g. 0.5% Tween or Triton), in addition to protease inhibitors, and 5%
glycerol without interfering with the ELISA?
Thank you for your suggestions,
Regina



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