Non-human factor Xa
Dr. David Martin
david.martin at read.my.sig
Thu Jan 16 11:32:58 EST 1997
In article <32DE5D1C.2BB3 at bilbo.bio.purdue.edu>,
alan friedman <afried at bilbo.bio.purdue.edu> wrote:
+>Does anyone know whether Factor Xa from non-humans can
+>be used for cleavage of Factor Xa linked fusion proteins?
Yes it can.
+>Are there protocols for making non-human Factor Xa?
You could try Barium precipitation followed by Q ion chromatography,
take the FX fractions and activate with Russels Viper Venom. You
could then rechromatograph to separate FXa from the other proteins
(You get a shift in elution profile on activation). It won't be pure
but will probably work OK (Though of course no guarantees are given)
* David Martin - Atherosclerosis and Thrombosis research group *
* http://www.uio.no/~damartin/ david.martin at biotek.uio.no *
* Lab +47 22 95 84 54 Fax +47 22 69 41 30 GSM +47 90 74 27 65 *
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