[Protein-analysis] Re: Protocol needed from study the protein aggregation

[Dr Engelbert Buxbaum]

Am 23.04.2008, 23:42 Uhr, schrieb Niraikulam Ayyadurai  
<microsathese from hotmail.com>:

>
> Hi all
> Recently i got a mutant gene and showing higher solubility then wild  
> type. we are assuming that our mutant preventing the aggregation of the  
> protein.
> I would like to study the thermal and chemical protein aggregation.
> i would be happy If any one provide me detailed protocol to study this  
> experiment.

Differential scanning calorimetry would be my method of choice. No point  
in posting the protocol, you need to find somebody with the required  
equiment to collaborate with. Plan on sending them plenty of protein  
(several 100 mg), there is a wide gap between what biophysicists and  
biochemists consider "a small sample". And biophysicists don't do protein  
purificantion and hence have absolutely no respect for the work you put in  
;-(