[Protein-analysis] Re: protein candidate

[Nick Theodorakis]

On May 15, 9:10 am, "assa sittner" <sitt... from lkb.ens.fr> wrote:
> Hi There,
> I need for some biochemical assay, a simple protein that fulfills the
> following requirements:
> 1. very stable (no significant degradation)
> 2. soluble
> 3. monomeric
> 4. Mw > 70 KDa
> 5. easily expressed and purified from E.coli
> 6. if possible, has an enzymatic activity that can be quantified
> is it too much to ask?
> ideas anyone?
>
> (I was thinking beta galactosidase, but couldn't find an article with nice
> gels that show the purity, and data about stability etc. is there something
> else?)
>
> have a nice day,

Beta-gal is, IIRC, a tetramer.

Maybe firefly luciferase; it's about 60-ish kDa, though. Don't know
how stable it is.

Glycogen phosphorylase? Myosin Light Chain Kinase? Gelatinase B?
They're all bigger than 70 kDa.

Nick

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Nick Theodorakis
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