[Protein-analysis] Re: Western Blot Formalin Fixed Tissue

[Dr Engelbert Buxbaum]

In article <mailman.35.1289798685.15153.proteins from net.bio.net>, 
r.zhang from auckland.ac.nz says...
> 
> I would like to know if it is possible to do western blotting experiment
> using formalin fixed frozen tissues of rat brain, thanks for that Rong

Difficult, as formalin cross-links proteins. The more common method is 
to rehydrate sections and elute proteins with hot RIPA-buffer containing 
detergents. The proteins are reduced, carboxymethylated and protease 
digested. The resulting peptides are separated by capillary 
electrophoresis and identified by MS/MS. For a review see Nirmalan et 
al.: Mol. Biosyst. 4 (2008) 712-20