From ijgmb from acadjourn.org Mon May 4 05:34:59 2009 From: ijgmb from acadjourn.org (International Journal of Genetics and Molecular Biology) Date: Mon May 4 11:00:05 2009 Subject: [Protein-analysis] call for papers Message-ID: *International Journal of Genetics and Molecular Biology* *www.academicjournals.org/IJGMB * Dear Colleague, *Introducing =91**International Journal of Genetics and Molecular Biology* = * (IJGMB)=92* The *International Journal of Genetics and Molecular Biology* (IJGMB) publishes high-quality solicited and unsolicited articles, in all areas of the subject. All articles published in IJGMB will be peer-reviewed. The following types of papers are considered for publication: =B7 Original articles in basic and applied research. =B7 Critical reviews, surveys, opinions, commentaries and essays. Our objective is to inform authors of the decision on their manuscript(s) within four weeks of submission. 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Best regards, *Bazim Adigwerex* Editorial Assistant International Journal of Genetics and Molecular Biology E-mail: ijgmb@acadjourn.org www.academicjournals.org/IJGMB From conferences from embl.de Tue May 5 21:07:19 2009 From: conferences from embl.de (EMBL Courses & Conferences) Date: Wed May 6 11:59:43 2009 Subject: [Protein-analysis] EMBO Conference Series on Protein Synthesis and Translational Control Message-ID: <200905060205.n4625Sb17279@net.bio.net> Dear Colleagues, EMBO Conference Series on Protein Synthesis and Translational Control Partnered with the "CSHL Meeting on Translational Control" EMBL Heidelberg, Germany, 9 - 13 September 2009 This is to alert you of the upcoming registration and abstract deadline 1 June 2009, which is less than 1 month away. There will be no deadline extension possible, because the organizers meet soon after the deadline to discuss the session allocations of the submitted abstracts. From the registrations that we have had already and based on the success of the 2005 and 2007 meetings here in Heidelberg, this will be a very popular meeting for which we will need to limit participation to a maximum of 300 attendees. The time of registration will be an admission criterium in case that the meeting is oversubscribed. We invite you to register and submit your abstract as soon as possible via http://www.embl.de/conferences/TransControl/2009 Looking forward to welcoming you in September! Anne, Marina, Nahum and Matt For further inquiries, please contact: EMBL Course and Conference Office Bettina Schaefer Meyerhofstr. 1 69117 Heidelberg email: schaefer@embl.de phone: +49-6221-387-8836 If you do not wish to receive any further information about events taking place at EMBL, please reply with "unsubscribe" in the subject. From sanjaysingh765 from gmail.com Fri May 15 06:04:09 2009 From: sanjaysingh765 from gmail.com (chunnu) Date: Fri May 15 11:27:08 2009 Subject: [Protein-analysis] gene expression study Message-ID: Hi all, I am interested to study the expression of a set of genes from rice and arabidopsis.Can any one tell me the simplest server/site to perform this analysis for me. regards chunnu From amanosz from yahoo.com Fri May 15 15:58:25 2009 From: amanosz from yahoo.com (David Liu) Date: Sat May 16 13:31:26 2009 Subject: [Protein-analysis] Contaminating Proteins for Fluorescence Polarization Assay Message-ID: <253604.7061.qm@web36507.mail.mud.yahoo.com> I am handing over some protein samples to a collaborator to do a fluorescence polarization assay to determine binding affinity as a check for activity of a protein that I'm modifying. Using quantitative Western or Coomassie Blue stain, I can determine the concentration of just the protein of interest in a mixture. My question is whether contaminating proteins (carrier protein, enzyme for modifications, etc) will affect the readout of the fluorescence polarization reading. I will lose a significant amount of modified protein if I purify it from the mixture, so I'm wondering if the "dirty" prep would work for fluorescence polarization if I know the concentration of the protein I'm interested in. Thanks From nadmsa1 from yahoo.com Tue May 19 16:06:50 2009 From: nadmsa1 from yahoo.com (nada mohamed) Date: Tue May 19 16:12:22 2009 Subject: [Protein-analysis] RNA LABELING Message-ID: <807994.17655.qm@web34406.mail.mud.yahoo.com> Dear all, ? I am a PhD student and I am studying RNA-Protein interaction. I need a protocol to label my RNA at 5'end with biotin, I found some researches using megascript kit to incorporate biotin and others using Maxiscript I am confused which one is suitable ? and is there any alternative methods, also what is the criteria that must be taken in account for RNA biotin labeling (to be sure that my RNA is not affected by biotin incorporation),?I mean the size of RNA the amount of?labeled?nucleotides. Any help will be appreciated.? ? Regards, ? PhD student @IMPERIAL COLLEGE LONDON. From engelbert_buxbaum from hotmail.com Fri May 22 13:11:21 2009 From: engelbert_buxbaum from hotmail.com (Dr Engelbert Buxbaum) Date: Fri May 22 19:29:34 2009 Subject: [Protein-analysis] Re: RNA LABELING References: Message-ID: Am 19.05.2009, 17:06 Uhr, schrieb nada mohamed : > I am a PhD student and I am studying RNA-Protein interaction. I need a > protocol to label my RNA at 5'end with biotin, I found some researches > using megascript kit to incorporate biotin and others using Maxiscript I > am confused which one is suitable ? and is there any alternative > methods, also what is the criteria that must be taken in account for RNA > biotin labeling (to be sure that my RNA is not affected by biotin > incorporation),?I mean the size of RNA the amount > of?labeled?nucleotides. Any help will be appreciated.? Don't use any kit until you have fully understood the method and can perform it with standard chemicals. Then kits can save you some time. Doing kits blindly however is a recipe for disaster.