a03m at biologie.uni-bremen.de (Georg Kroeger)
on the Subject: Tetrahymena Nuclei-separation
(13 Jun 1995 12:51:51 GMT) wrote:
> Dear Colleagues,
>> I tried several protocols to isolate Mic- and Mac-Nuclei from Tetrahymena
> thermophila. But none of them brought me to success. Could anybody give me
> an advice to isolate and separate these Nuclei without plenty of
> centrifugation-steps.
>> Carsten
>Carsten:
If your Tetrahymena macronuclei are large enough they can be
separated from other organelles in an homogenate by filtering through
a 20 um nickel mesh as done with Stylonychia by Schlegel et al.
Chromosoma 99: 401.
-Dennis Goode
Dept Zool
UMD, College Park 20742
