From BIOSCI-REQUEST Fri Feb 10 07:29:54 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.9/8.6.6) id HAA05775 for rust-mil-list; Fri, 10 Feb 1995 07:29:54 -0800 Received: from sun2.mhs-relay.ac.uk (sun2.mhs-relay.ac.uk [128.86.8.35]) by net.bio.net (8.6.9/8.6.6) with SMTP id HAA05769 for ; Fri, 10 Feb 1995 07:29:50 -0800 X400-Received: by mta sun2.mhs-relay.ac.uk in /PRMD=uk.ac/ADMD= /C=gb/; Relayed; Fri, 10 Feb 1995 15:29:00 +0000 X400-Received: by mta arcs.afrc.ac.uk in /PRMD=UK.AC/ADMD= /C=GB/; Relayed; Fri, 10 Feb 1995 15:29:08 +0000 X400-Received: by /PRMD=UK.AC/ADMD= /C=GB/; converted (ia5 text (2)); Relayed; Fri, 10 Feb 1995 15:24:26 +0000 Date: Fri, 10 Feb 1995 15:24:26 +0000 X400-Originator: james.brown@afrc.ac.uk X400-Recipients: rust-mil@net.bio.net X400-MTS-Identifier: [/PRMD=UK.AC/ADMD= /C=GB/;2937221510021995/A22386/JIIS] X400-Content-Type: P2-1988 (22) Content-Identifier: 119253D62200 From: James Brown Message-ID: <2937221510021995/A22386/JIIS/119253D62200*@MHS> To: rust-mil@net.bio.net (Non Receipt Notification Requested) Subject: spring wheat Sensitivity: Private I sent this message yesterday, but it appears to have disappeared into cyberspace. Apologies if you get it twice. ______________________________________________________________________ My Ph.D. student, Bob O'Hara, and I are looking for a spring wheat variety to grow in a field trial this year. We need one which is susceptible to mildew but at least moderately resistant to brown rust, and preferably to yellow rust too. All of the currently recommended UK spring wheats are resistant to mildew, with the exception of Tonic, which is quite susceptible to mildew, but not to the races that we would want to infect the trial with. Do you know of any suitable variety? We would need to buy (or otherwise obtain) about 2 kg of it. James Brown _______________________________________________________ Dr J. K. M. Brown, Cereals Research Department, John Innes Centre, Colney Lane, Norwich, NR4 7UH, England Phone: (+44)(0) 1603 452571. Fax: (+44)(0) 1603 502241. E-mail: james.brown@bbsrc.ac.uk From BIOSCI-REQUEST Fri Feb 10 10:05:25 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.9/8.6.6) id KAA11680 for rust-mil-list; Fri, 10 Feb 1995 10:05:25 -0800 Received: from sun2.mhs-relay.ac.uk (sun2.mhs-relay.ac.uk [128.86.8.35]) by net.bio.net (8.6.9/8.6.6) with SMTP id KAA11675 for ; Fri, 10 Feb 1995 10:05:20 -0800 X400-Received: by mta sun2.mhs-relay.ac.uk in /PRMD=uk.ac/ADMD= /C=gb/; Relayed; Fri, 10 Feb 1995 18:04:06 +0000 X400-Received: by mta arcs.afrc.ac.uk in /PRMD=UK.AC/ADMD= /C=GB/; Relayed; Fri, 10 Feb 1995 16:01:26 +0000 X400-Received: by /PRMD=UK.AC/ADMD= /C=GB/; converted (ia5 text (2)); Relayed; Thu, 9 Feb 1995 16:56:37 +0000 Date: Thu, 9 Feb 1995 16:56:37 +0000 X400-Originator: james.brown@afrc.ac.uk X400-Recipients: rust-mil@net.bio.net X400-MTS-Identifier: [/PRMD=UK.AC/ADMD= /C=GB/;5919551609021995/A17674/JIIS] X400-Content-Type: P2-1988 (22) Content-Identifier: 11924C371100 From: James Brown Message-ID: <5919551609021995/A17674/JIIS/11924C371100*@MHS> To: rust-mil@net.bio.net (Non Receipt Notification Requested) Subject: spring wheat Sensitivity: Private My Ph.D. student, Bob O'Hara, and I would like to find a variety of spring wheat which is susceptible to mildew but not to brown rust, and preferably not to yellow rust, for use in a field trial this year. The current British varieties are rated as resistant to very resistant by the National Institute of Agricultural Botany, apart from Tonic, which is generally quite susceptible but unfortunately not to the races which we would want to infect the trial with. Do you know of any such variety? We would need to buy (or otherwise obtain) about 2 kg. James Brown _____________________________________________________ Dr J. K. M. Brown, Cereals Research Department, John Innes Centre, Colney Lane, Norwich, NR4 7UH, England Phone: (+44)(0)1603 452571. Fax: (+44)(0)1603 502241. E-mail: james.brown@bbsrc.ac.uk From BIOSCI-REQUEST Thu Feb 16 14:13:20 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.9/8.6.6) id OAA08792 for rust-mil-list; Thu, 16 Feb 1995 14:13:20 -0800 Received: from morgan.angis.su.OZ.AU (morgan.angis.su.OZ.AU [129.78.25.38]) by net.bio.net (8.6.9/8.6.6) with ESMTP id OAA08761 for ; Thu, 16 Feb 1995 14:13:15 -0800 Received: (from pbic00@localhost) by morgan.angis.su.OZ.AU (8.6.8.1/8.6.6) id JAA21561 for rust-mil@net.bio.net; Fri, 17 Feb 1995 09:13:12 +1100 Date: Fri, 17 Feb 1995 09:13:12 +1100 From: "Plant Breeding Inst." Message-Id: <199502162213.JAA21561@morgan.angis.su.OZ.AU> To: rust-mil@net.bio.net Content-Length: 2210 16 February, 1995 Dear Rust/Mildew Colleague Re: Wheat Gene Pm8 The 1BL.1RS and 1B(1R) introgression to wheat has been the subject of many research projects and there is still more to come. I have been interested in the report that only some lines of the group are resistant to cultures avirulent on seedlings with Pm8 as might be shown in tests on seedlings of, for example, Kavkaz, Aurora or Skorospelka 35. Veery selections and a number of CIMMYT wheats, as well as certain European wheats, are susceptible with such cultures. Of course there are reports of virulence for Pm8 where seedlings of Kavkaz, etc., would be susceptible. We tested SSD lines of Kohinoor (a Pakistani wheat) and Parula (a CIMMYT line) which are resistant to the local mildew population at Cobbitty, crossed with a Condor Selection (susceptible) and found that about 50% of lines with Lr26 (1B.1R) were resistant, The other 50% and all lines lacking 1B.1R were susceptible. These results indicate that the mildew results must be in (or closely linked to) the rye segment and that there is genetic suppression of resistance - possibly by a single gene. We are now planning crosses to isolate the suppressor and to locate the chromosome involved. This is not our "true line of work", but I believe the observations are so interesting and potentially important that the work should be done. Can anyone help me with the following questions: - is similar work going on elsewhere - does anyone have results they would like to share - is it possible to produce a catalogue of 1B.1R wheats which have Pm8 active and those with Pm8 suppressed - is it possible that wheats with a suppressed Pm8 based on seedling tests will display plant resistance to the same pathotypes. My impression is that Veery, for example, is resistant in our field nurseries, although I must examine more records and carry out a formal test. - one person who may be able to provide some information is Manfred Heun - what is his current address - postal or electronic - copies of this message will be sent also to F. Zeller and S. Leath. Thank you for reading it and for any help. Regards. BOB McINTOSH From BIOSCI-REQUEST Thu Feb 23 06:09:17 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.9/8.6.6) id GAA26431 for rust-mil-list; Thu, 23 Feb 1995 06:09:17 -0800 Received: from biobase.dk (biobase.dk [130.225.13.135]) by net.bio.net (8.6.9/8.6.6) with SMTP id GAA26426 for ; Thu, 23 Feb 1995 06:09:10 -0800 From: andersc@biobase.dk Received: by biobase.dk; id AA29969; Thu, 23 Feb 1995 15:08:55 +0100 Message-Id: <9502231408.AA29969@biobase.dk> To: rust-mil@net.bio.net Cc: andersc@biobase.dk Subject: Coleoptiles -Growth/Mildew Date: Thu, 23 Feb 95 15:08:55 +0100 X-Mts: smtp Hi everybody! I am working with defense response genes from barley. Our model system is powdery mildew/barley. I am interested in setting up inoculation of barley coleoptiles with powdery mildew to test whether the molecular processes in this tissue resemble the resistance processes observed in leaves. 1) Does anybody know of a good method to inoculate and grow coleoptiles (Or a reference to such a method)?. 2) Is the interaction types in coleoptiles the same as the interaction types on leaves?. If not -- Which barley lines should I use, and to which mildew isolates, to get compatible, hypersensitive response and papilla response in coleoptiles? . Any hint which might answer some of these questions are appreciated. Anders B. Christensen, Agricultural University, Denmark. Andersc@biobase.dk From BIOSCI-REQUEST Thu Feb 23 09:42:50 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.9/8.6.6) id JAA06357 for rust-mil-list; Thu, 23 Feb 1995 09:42:50 -0800 Received: from sun2.mhs-relay.ac.uk (sun2.mhs-relay.ac.uk [128.86.8.35]) by net.bio.net (8.6.9/8.6.6) with SMTP id JAA06354 for ; Thu, 23 Feb 1995 09:42:46 -0800 X400-Received: by mta sun2.mhs-relay.ac.uk in /PRMD=uk.ac/ADMD= /C=gb/; Relayed; Thu, 23 Feb 1995 17:40:40 +0000 X400-Received: by mta arcs.afrc.ac.uk in /PRMD=UK.AC/ADMD= /C=GB/; Relayed; Thu, 23 Feb 1995 16:37:25 +0000 X400-Received: by /PRMD=UK.AC/ADMD= /C=GB/; converted (ia5 text (2)); Relayed; Thu, 23 Feb 1995 16:33:58 +0000 Date: Thu, 23 Feb 1995 16:33:58 +0000 X400-Originator: james.brown@afrc.ac.uk X400-Recipients: rust-mil@net.bio.net X400-MTS-Identifier: [/PRMD=UK.AC/ADMD= /C=GB/;9728331623021995/A12819/JII] X400-Content-Type: P2-1988 (22) Content-Identifier: 1192BC211800 From: James Brown (Tel ) Message-ID: <9728331623021995/A12819/JII/1192BC211800*@MHS> To: rust-mil@net.bio.net (Receipt Notification Requested) (Non Receipt Notification Requested) Subject: Re: Coleoptiles -Growth/Mildew Sensitivity: Private ! Anders B. Christensen, Agricultural University, Denmark, wrote: ! I am working with defense response genes from barley. Our ! model system is powdery mildew/barley. I am interested in ! setting up inoculation of barley coleoptiles with powdery ! mildew to test whether the molecular processes in this ! tissue resemble the resistance processes observed in ! leaves. ! 1) Does anybody know of a good method to inoculate and grow ! coleoptiles (Or a reference to such a method)?. Jim Aist's group at Cornell did this to study the expression of mlo. Two relevant papers are by Bayles & Aist (1987) Phys Mol Pl Path 30:337-345 and Gold et al (1986) Phys Mol Pl Path 29:115-129. ! 2) Is the interaction types in coleoptiles the same as the ! interaction types on leaves?. A very interesting question, but I don't know! ! If not -- Which barley lines should I use, and to which ! mildew isolates, to get compatible, hypersensitive response ! and papilla response in coleoptiles?. You would get a papilla response from an mlo line. We've studied four race-specific genes, Mla1, Mla3, Mla6, Mla7, which respond to avirulent largely by a hypersensitive response. Essentially, Mla1 and Mla6 lines express hypersensitivity in the epidermis and Mla7 and Mla3 lines in both epidermis and mesophyll (paper by Boyd, Smith, Foster & Brown in press). Golden Promise and Carlsberg II (which both have Mla8) are widely used as susceptible, control varieties. A wide range of isolates, which have avirulence towards at least these Mla genes, should be suitable for your studies. James Brown From BIOSCI-REQUEST Mon Feb 27 13:24:01 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.10/8.6.6) id NAA24690 for rust-mil-list; Mon, 27 Feb 1995 13:24:01 -0800 Received: from morgan.angis.su.OZ.AU (morgan.angis.su.OZ.AU [129.78.25.38]) by net.bio.net (8.6.10/8.6.6) with ESMTP id NAA24685 for ; Mon, 27 Feb 1995 13:23:53 -0800 Received: (from pbic00@localhost) by morgan.angis.su.OZ.AU (8.6.10/8.6.6) id IAA01552 for rust-mil@net.bio.net; Tue, 28 Feb 1995 08:23:48 +1100 Date: Tue, 28 Feb 1995 08:23:48 +1100 From: "Plant Breeding Inst." Message-Id: <199502272123.IAA01552@morgan.angis.su.OZ.AU> To: rust-mil@net.bio.net Content-Length: 1813 TO: Rust-mil@net.bio.net 1. Suppression of Pm8 Thanks to several people for communicating information and suggestions - they were helpful and I have since replied to several of you. There is a problem that I am not sure how to handle. In replying directly to me, part of an interesting discussion is not made available to others. On the other hand, colleagues may wish to pass on information relevant to an enquiry when they would prefer that the information not be made widely available. May I suggest: (a) General replies should be passed back through the group (b) Information for limited circulation be passed back to the original enquirer. If total confidentiality is required, this should be stated. In my case, at least I would like to summarise the comments I received for sharing with the group because genetic suppression of resistance genes is an interesting and important topic, just when resistance genes are being cloned and sequenced. Some guidance James Brown? 2. Reply to Anders Christensen/Coleoptiles - growth/mildew You asked if coleoptiles respond the same way as leaves. From my limited experience with intact wheat seedlings - I would say yes. One very clear exception has been near-isogenic lines with Pm3a. The primary leaves of Pm3a lines are usually immune except for an occasional 'leaky' pustule that does not involve virulence, but colonies on the coleoptiles can sporulate quite profusely. In this case, there is a clear difference. Suggest you look at intact seedlings prior to making any assumptions. 3. Question to James Brown. Can you post the circulation list at intervals? It seems to have grown considerably since the last list I saw. It is important to know who is not on the list as well as who is on it. Bob McIntosh From BIOSCI-REQUEST Tue Feb 28 23:28:22 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.10/8.6.6) id GAA12702 for rust-mil-list; Tue, 28 Feb 1995 06:19:45 -0800 Received: from abru.cg.com (abru.cg.com [199.98.86.3]) by net.bio.net (8.6.10/8.6.6) with SMTP id GAA12698 for ; Tue, 28 Feb 1995 06:19:42 -0800 Received: with SMTP-MR; Tue, 28 Feb 1995 14:12:20 EST MR-Received: by mta YOMAMA.MUAS; Relayed; Tue, 28 Feb 1995 14:12:20 -0500 (EST) MR-Received: by mta YOMAMA; Relayed; Tue, 28 Feb 1995 09:12:20 -0500 (EST) Disclose-recipients: prohibited Date: Tue, 28 Feb 1995 14:12:20 EST From: DELANEY 919-541-8577 Subject: RE: - no subject (01HNJUJPQ9H89D5YIQ) - In-reply-to: <199502272123.IAA01552@morgan.angis.su.OZ.AU> To: Rust-mil@net.bio.net, "Plant Breeding Inst." Message-id: <8820120928021995/A47625/YOMAMA/1192E24C1200*@MHS> Autoforwarded: false MIME-version: 1.0 Content-type: TEXT/PLAIN Importance: normal Priority: normal Sensitivity: Company-Confidential UA-content-id: 1192E24C1200 X400-MTS-identifier: [;8820120928021995/A47625/YOMAMA] Hop-count: 0 Bob McIntosh poses the question of how to fairly share responses he received to a query he posted on Rust-mil@net.bio.net. He points out that some respondents may not wish to share their replies with the entire network. I suggest that Rust-mil@net.bio.net follow the same general policy that has evolved in other bionet newsgroups. The informal policy, is that the person who requests information from the network, compile and later re-post a summary of responses received. This benefits the whole group, and encourages discussion. If someone wishes their response to be confidential, they simply need to state it in their reply. Of course, posting replies to the entire bulletin board is appropriate, if the content seems of general interest. Terry Delaney Ciba Ag Biotech