From BIOSCI-REQUEST Mon Mar 6 08:32:41 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.10/8.6.6) id IAA24713 for rust-mil-list; Mon, 6 Mar 1995 08:32:41 -0800 Received: from hearnvax.nic.surfnet.nl (hearnvax.nic.surfnet.nl [192.87.5.131]) by net.bio.net (8.6.10/8.6.6) with ESMTP id IAA24709 for ; Mon, 6 Mar 1995 08:32:37 -0800 From: G.H.J.KEMA@ipo.agro.nl Received: from AGRO02 by HEARNVAX.nic.SURFnet.nl (PMDF V4.2-12 #3330) id <01HNTO8E97WG00C10C@HEARNVAX.nic.SURFnet.nl>; Mon, 6 Mar 1995 17:34:07 +0100 (MET) Received: from IPOALP by AGRO.NL (PMDF V4.2-12 #4885) id <01HNTO1BPLV4002DMQ@AGRO.NL>; Mon, 6 Mar 1995 17:28:24 MET Received: from IPO.AGRO.NL by IPO.AGRO.NL (PMDF V4.3-7 #7552) id <01HNTO5BCHVK000BKR@IPO.AGRO.NL>; Mon, 6 Mar 1995 17:31:37 GMT Date: Mon, 06 Mar 1995 17:31:37 +0000 (GMT) Subject: roy Johnson To: rust-mil@net.bio.NET Message-id: <01HNTO5BCITE000BKR@IPO.AGRO.NL> X-Envelope-to: rust-mil@net.bio.NET X-VMS-To: rust MIME-version: 1.0 Content-type: TEXT/PLAIN; CHARSET=US-ASCII Content-transfer-encoding: 7BIT Dear colleagues, Tomorrow March 7 Roy Johnson at the JICentre, Norwich, UK, retires from working on, primarily, yellow rust of wheat. It would probably be a good idea to "abuse" the network to pass the best wishes to Roy at the verge of his active and fruitful career. So let's fill up his mail buffer ! Best wishes, Gert HJ Kema, DLO-Research Institute for Plant Protection, Wageningen, The Netherlands From BIOSCI-REQUEST Tue Mar 7 18:04:38 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.10/8.6.6) id SAA08117 for rust-mil-list; Tue, 7 Mar 1995 18:04:38 -0800 Received: from sun2.mhs-relay.ac.uk (sun2.mhs-relay.ac.uk [128.86.8.35]) by net.bio.net (8.6.10/8.6.6) with SMTP id SAA08111 for ; Tue, 7 Mar 1995 18:04:32 -0800 X400-Received: by mta sun2.mhs-relay.ac.uk in /PRMD=uk.ac/ADMD= /C=gb/; Relayed; Wed, 8 Mar 1995 02:03:08 +0000 X400-Received: by mta arcs.bbsrc.ac.uk in /PRMD=UK.AC/ADMD= /C=GB/; Relayed; Wed, 8 Mar 1995 01:48:33 +0000 X400-Received: by /PRMD=UK.AC/ADMD= /C=GB/; converted (ia5 text (2)); Relayed; Tue, 7 Mar 1995 09:55:56 +0000 Date: Tue, 7 Mar 1995 09:55:56 +0000 X400-Originator: james.brown@bbsrc.ac.uk X400-Recipients: rust-mil@net.bio.net X400-MTS-Identifier: [/PRMD=UK.AC/ADMD= /C=GB/;2721550907031995/A16931/JIIS] X400-Content-Type: P2-1988 (22) Content-Identifier: 11933A771200 From: James Brown Message-ID: <2721550907031995/A16931/JIIS/11933A771200*@MHS> To: Rust-mildew (Non Receipt Notification Requested) Subject: Roy Johnson; replying to messages; subject line Sensitivity: Private Following Gert Kema's message, if you'd like to send Roy your congratulations on his "retirement" - it looks as though he'll be just as busy as before - his e-mail address is Roy.Johnson@bbsrc.ac.uk Replying to messages -------------------- To respond to Bob McIntosh's message about posting replies to questions, I largely agree with Terry Delaney's suggestion. Firstly, when you reply to a message which comes from the Bionet Rust-Mil group, your mailing system will ask if you want to your reply to the person who posted the question or to the whole group. As Terry said, replying to the person who asked the question is appropriate if your reply is confidential or if the information which you're providing is essentially personal. However, if your reply may be of interest to at least some additional members of the group, and isn't confidential, please feel free to reply to the group. If you receive replies to a question which may be of interest to others, as Bob McIntosh seems to have done, do please summarise them (or compile them into a single message) and post them to the group. It's up to you to leave out confidential information. Personally, I find the subject of suppression of resistance genes (and of avirulence genes?) very interesting and I'd very much like to hear what others might have to say on this subject. Circulation list ---------------- To reply to another question from Bob McIntosh, > Can you post the circulation list at intervals? It seems to > have grown considerably since the last list I saw. It is > important to know who is not on the list as well as who is on > it. When the list first started, I asked Dave Kristofferson, who runs Bionet, if I could have a list of the names of people on the list. This was part of his reply: > I include the current list below. It has been our longstanding policy > not to make mailing lists public, but we do provide them to the > discussion leaders for their reference. Our philosophy has always > been that people should be consulted before their addresses are made > public, even though this philosophy is at odds with many other mailing > list policies. Please note, then that we provide this list for your > reference and will provide it as often as you wish (we can set up an > automatic mailer on some periodic basis to you). However, we would > appreciate it if you would ask people on the list first before > distributing it further. What I suggest is that if you DON'T want your e-mail address to be publicised, please send me an e-mail to say so. (PLEASE don't e-mail me just to say that you don't mind your address being publicised, because our poor mailing system would choke on the number of messages.) I would then be happy to ask Dave Kristofferson if he would have any objections to my distributing a list of names of people on Rust-Mil. If he doesn't have any such objections, I'll post the list. Subject lines ------------- Please include a subject line in your e-mail, to help recipients know whether or not the mail is of interest to them. James Brown From BIOSCI-REQUEST Fri Mar 10 01:32:46 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.10/8.6.6) id BAA24347 for rust-mil-list; Fri, 10 Mar 1995 01:32:46 -0800 Received: from hearnvax.nic.surfnet.nl (hearnvax.nic.surfnet.nl [192.87.5.131]) by net.bio.net (8.6.10/8.6.6) with ESMTP id BAA24342 for ; Fri, 10 Mar 1995 01:32:36 -0800 From: G.H.J.KEMA@ipo.agro.nl Received: from AGRO02 by HEARNVAX.nic.SURFnet.nl (PMDF V4.2-12 #3330) id <01HNYUQXQD9S00C10C@HEARNVAX.nic.SURFnet.nl>; Fri, 10 Mar 1995 10:34:57 +0100 (MET) Received: from IPOALP by AGRO.NL (PMDF V4.2-12 #4885) id <01HNYUL24N80000AIM@AGRO.NL>; Fri, 10 Mar 1995 10:30:05 MET Received: from IPO.AGRO.NL by IPO.AGRO.NL (PMDF V4.3-7 #7552) id <01HNYUNYU0WW000D6D@IPO.AGRO.NL>; Fri, 10 Mar 1995 10:32:25 GMT Date: Fri, 10 Mar 1995 10:32:25 +0000 (GMT) Subject: supression of resistance To: rust-mil@net.bio.NET Message-id: <01HNYUNYU1V6000D6D@IPO.AGRO.NL> X-Envelope-to: rust-mil@net.bio.NET X-VMS-To: rust MIME-version: 1.0 Content-type: TEXT/PLAIN; CHARSET=US-ASCII Content-transfer-encoding: 7BIT Following James Brown's message I would like to inform the group on the results that we obtained at IPO-DLO with respect to suppression of resistance. I agree with James that this is a topic for an interesting discussion. It seems that quite a number of people have observed and still observe the phenomenon but it has not been reported that much. We did our research using synthetic hexaploids that were derived from T. turgidum dicoccoides (2n=28, AABB) and Aegilops squarrosa (2n=14, DD). The wild emmer accessions had been studied by Cor van Silfhout in the past so their response to a number of yellow rust races was known. The majority of these accession repond with hypersensitive flecks (IT1-2 on the 0-9 scale). There was not much known about the resistance to YR in Ae. squarrosa so we tested some 30 accessions with 10 Yr races (not published). Since we knew the responses of both species toward a number of YR races, we were interested in the response of the synthetic hexaploids. Henceforward parents and synthetic hexaploids weretested with five Yr races in the seedling stage and with two of them in the adult plant stage. Our results showed that in most of the responses the resistance level of one or both parents was significantly suppressed (usually up to IT7 or sometimes IT 8). It was also evident that suppression did not operate toward all the races and also not in both growth stages. In other words there was specificity of the suppression. It is indeed very curious that challenging a host, which has one (e.g. Yr15) or even more resistance genes from the donor species combined in the synthetic hexaploid, with an avirulent race for those genes, reveals susceptible responses, which does not seem to be in line with the GFG relationship. We have written a publication (Phytopathology, in press) on the aforementioned data and put forward an hypothesis there which is in line with the GFG relationship. It might stimulate discussion to copy part of that discussion in this message. " In our study susceptible responses developed slowly in the seedling as well as in the adult plant stage and were only occasionally beyond IT 7. We also observed that susceptible ITs can develop from an initial resistant or intermediate response (!), which might imply that elicitation of resistance is restricted to specific stages of fungal development in the host. Williams et al. (44) suggested that envi ronmental fluctuation affected the expression of the stem rust resistance gene, and thus of the suppressor gene involved in their study. Our data provide evidence for different modes of timing of suppressor and resistance genes comparing responses at 16 dpi and 21 dpi), rather than environmental fluctuations, to be responsible for heterogeneous expression of the resistance genes. Tepper & Anderson (36) emphasized the importance of gene regulation and its possible role in race-cultivar specificity rather than the direct interaction between the products of avirulence and resistance genes, elicitors and receptors, respectively, though being a primary aspect of host-pathogen interaction models (6,7,8,9,11). Suppressors might be analogues of such regulator products that control timing and expression of resistance. Certainly suppressors of resistance do interfere with the expression of resistance genes gene transferred from alien species, but they could also have a more general function in gene expression." There are several options to think about hte mechanism of suppression activation. The products could for instance occupy receptor sites so that elicitors cannot trigger R genes anymore. However this does not explain specificity and is also not in line with the observation that initially an R response is triggered. I would be interested to discuss the hypothesis that suppressor genes synthesize regulator products, and could be activated by a product of the R genes. Such a hypothesis possibly provides an explanation for heterogeneous responses in synthetic hexaploids, being the result of stochastic events that may eventually lead to the different modes of expression of the resistance, running from true hypersensitivity to susceptibility, including mesothetic responses, and is supplementing a model for host-parasite interaction as proposed by Britten & Davidson (1969) that was discussed by Day (1974). We also crossed synthetic hexaploids with the same wild emmer parent but different Aegilops squarrosa parents in order to investigate the genetics of suppression. The wild emmer parent was resistant and the Aegilops squarrosa parents were susceptible to the applied race. The resistance of the wild emmer parent was suppressed in both synthetic hexaploids. Thus all the segregation for infection types was considered to be due to segregating suppressor factors. Well, there was sufficient segregation, and the inheritance of the suppressors did not seem to be simple. What is of interest here also is that we crossed two susceptible parents (the synthetic hexaploids) and observed plants with resistant ITs in the segregating population. A good example of transgression. Since it is known that suppressor factors also occurred in commercial USA wheats, I am interested in the opinion of the group that suppressors might be (partly) responsible for transgressive From BIOSCI-REQUEST Sun Mar 12 11:37:50 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.10/8.6.6) id FAA02606 for rust-mil-list; Wed, 8 Mar 1995 05:15:18 -0800 Received: from rishp1.risoe.dk (rishp1.risoe.dk [130.226.48.13]) by net.bio.net (8.6.10/8.6.6) with ESMTP id FAA02591 for ; Wed, 8 Mar 1995 05:15:11 -0800 Received: from risrms1.risoe.dk by rishp1.risoe.dk with ESMTP (1.37.109.11/16.2) id AA077778643; Wed, 8 Mar 1995 14:17:23 +0100 Received: from RISRMS1/SpoolDir by risrms1.risoe.dk (Mercury 1.20); 8 Mar 95 14:14:21 +0100 Received: from SpoolDir by RISRMS1 (Mercury 1.20); 8 Mar 95 14:13:49 +0100 From: "Hanne Ostergard" Organization: Risoe National Laboratory, Denmark To: rust-mil@net.bio.net Date: Wed, 8 Mar 1995 14:13:44 MET Subject: COST 817: Short-Term Scientific Missions Priority: normal X-Mailer: Pegasus Mail/Windows (v1.22) Message-Id: <37B7407678A@risrms1.risoe.dk> To participants in COST 817, This to remind you that the deadline for applications for Short-Term Scientific Missions is 20'th of March. The Application Form and Guidelines can be requested from the national delegates of the Management Committee, from Prof. Masson in Brussels or from myself. Note that the objectives of the mission should be to learn a new technique (broadly speaking) or to make measurements (or analyses) using instruments or methods not available in your own lab. The duration should be between 3 days and 1 month and the maximum amount to be requested is 1500 ECU per mission. Only scientists from labs actively participating in the COST Action can apply. --------------------------- Hanne Oestergaard Environmental Science and Technology Department Risoe National Laboratory Post Box 49 DK-4000 Roskilde Denmark Direct phone: 45 4677 4111 --------------------------- From BIOSCI-REQUEST Sun Mar 12 12:03:16 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.10/8.6.6) id FAA02400 for rust-mil-list; Wed, 8 Mar 1995 05:04:29 -0800 Received: from hearnvax.nic.surfnet.nl (hearnvax.nic.surfnet.nl [192.87.5.131]) by net.bio.net (8.6.10/8.6.6) with ESMTP id FAA02397 for ; Wed, 8 Mar 1995 05:04:18 -0800 From: G.H.J.KEMA@ipo.agro.nl Received: from AGRO02 by HEARNVAX.nic.SURFnet.nl (PMDF V4.2-12 #3330) id <01HNW9JQHJU800E5G6@HEARNVAX.nic.SURFnet.nl>; Wed, 8 Mar 1995 14:06:35 +0100 (MET) Received: from IPOALP by AGRO.NL (PMDF V4.2-12 #4885) id <01HNW9CN4QHC0044NO@AGRO.NL>; Wed, 8 Mar 1995 14:00:49 MET Received: from IPO.AGRO.NL by IPO.AGRO.NL (PMDF V4.3-7 #7552) id <01HNW9GN8ZCW000BVY@IPO.AGRO.NL>; Wed, 8 Mar 1995 14:04:02 GMT Date: Wed, 08 Mar 1995 14:04:02 +0000 (GMT) Subject: suppression of resistance To: rust-mil@net.bio.net Message-id: <01HNW9GN90AQ000BVY@IPO.AGRO.NL> X-Envelope-to: rust-mil@net.bio.NET X-VMS-To: rust MIME-version: 1.0 Content-type: TEXT/PLAIN; CHARSET=US-ASCII Content-transfer-encoding: 7BIT Following James Brown's message I would like to inform the group on the results that we obtained at IPO-DLO with respect to suppression of resistance. I agree with James that this is a topic for an interesting discussion. It seems that quite a number of people have observed and still observe the phenomenon but it has not been reported that much. We did our research using synthetic hexaploids that were derived from T. turgidum dicoccoides (2n=28, AABB) and Aegilops squarrosa (2n=14, DD). The wild emmer accessions had been studied by Cor van Silfhout in the past so their response to a number of yellow rust races was known. The majority of these accession repond with hypersensitive flecks (IT1-2 on the 0-9 scale). There was not much known about the resistance to YR in Ae. squarrosa so we tested some 30 accessions with 10 Yr races (not published). Since we knew the responses of both species toward a number of YR races, we were interested in the response of the synthetic hexaploids. Henceforward parents and synthetic hexaploids weretested with five Yr races in the seedling stage and with two of them in the adult plant stage. Our results showed that in most of the responses the resistance level of one or both parents was significantly suppressed (usually up to IT7 or sometimes IT 8). It was also evident that suppression did not operate toward all the races and also not in both growth stages. In other words there was specificity of the suppression. It is indeed very curious that challenging a host, which has one (e.g. Yr15) or even more resistance genes from the donor species combined in the synthetic hexaploid, with an avirulent race for those genes, reveals susceptible responses, which does not seem to be in line with the GFG relationship. We have written a publication (Phytopathology, in press) on the aforementioned data and put forward an hypothesis there which is in line with the GFG relationship. It might stimulate discussion to copy part of that discussion in this message. " In our study susceptible responses developed slowly in the seedling as well as in the adult plant stage and were only occasionally beyond IT 7. We also observed that susceptible ITs can develop from an initial resistant or intermediate response (!), which might imply that elicitation of resistance is restricted to specific stages of fungal development in the host. Williams et al. (44) suggested that envi ronmental fluctuation affected the expression of the stem rust resistance gene, and thus of the suppressor gene involved in their study. Our data provide evidence for different modes of timing of suppressor and resistance genes comparing responses at 16 dpi and 21 dpi), rather than environmental fluctuations, to be responsible for heterogeneous expression of the resistance genes. Tepper & Anderson (36) emphasized the importance of gene regulation and its possible role in race-cultivar specificity rather than the direct interaction between the products of avirulence and resistance genes, elicitors and receptors, respectively, though being a primary aspect of host-pathogen interaction models (6,7,8,9,11). Suppressors might be analogues of such regulator products that control timing and expression of resistance. Certainly suppressors of resistance do interfere with the expression of resistance genes transferred from alien species, but they could also have a more general function in gene expression." There are several options to think about hte mechanism of suppression activation. The products could for instance occupy receptor sites so that elicitors cannot trigger R genes anymore. However this does not explain specificity and is also not in line with the observation that initially an R response is triggered. I would be interested to discuss the hypothesis that suppressor genes synthesize regulator products, and could be activated by a product of the R genes. Such a hypothesis possibly provides an explanation for heterogeneous responses in synthetic hexaploids, being the result of stochastic events that may eventually lead to the different modes of expression of the resistance, running from true hypersensitivity to susceptibility, including mesothetic responses, and is supplementing a model for host-parasite interaction as proposed by Britten & Davidson (1969) that was discussed by Day (1974). We also crossed synthetic hexaploids with the same wild emmer parent but different Aegilops squarrosa parents in order to investigate the genetics of suppression. The wild emmer parent was resistant and the Aegilops squarrosa parents were susceptible to the applied race. The resistance of the wild emmer parent was suppressed in both synthetic hexaploids. Thus all the segregation for infection types was considered to be due to segregating suppressor factors. Well, there was sufficient segregation, and the inheritance of the suppressors did not seem to be simple. What is of interest here also is that we crossed two susceptible parents (the synthetic hexaploids) and observed plants with resistant ITs in the segregating population. A good example of transgression. Since it is known that suppressor factors also occurred in commercial USA wheats, I am interested in the opinion of the group that suppressors might be (partly) responsible for transgressive segregation (see also Wallwork & Johnson) For us the mechanism and genetics of suppression are of particular interest and I would appreciate any reply to discuss the phenomenon and the approach to study it further. From BIOSCI-REQUEST Thu Mar 16 18:43:18 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.11/8.6.6) id FAA20569 for rust-mil-list; Thu, 16 Mar 1995 05:52:32 -0800 Received: from sun2.mhs-relay.ac.uk (sun2.mhs-relay.ac.uk [128.86.8.35]) by net.bio.net (8.6.11/8.6.6) with SMTP id FAA20549 for ; Thu, 16 Mar 1995 05:52:20 -0800 X400-Received: by mta sun2.mhs-relay.ac.uk in /PRMD=uk.ac/ADMD= /C=gb/; Relayed; Thu, 16 Mar 1995 13:52:06 +0000 X400-Received: by mta arcs.bbsrc.ac.uk in /PRMD=UK.AC/ADMD= /C=GB/; Relayed; Thu, 16 Mar 1995 13:55:04 +0000 X400-Received: by /PRMD=UK.AC/ADMD= /C=GB/; converted (ia5 text (2)); Relayed; Thu, 16 Mar 1995 13:50:50 +0000 Date: Thu, 16 Mar 1995 13:50:50 +0000 X400-Originator: james.brown@bbsrc.ac.uk X400-Recipients: rust-mil@net.bio.net X400-MTS-Identifier: [/PRMD=UK.AC/ADMD= /C=GB/;5200501316031995/A35063/JIIS] X400-Content-Type: P2-1988 (22) Content-Identifier: 119383713A00 From: James Brown Message-ID: <5200501316031995/A35063/JIIS/119383713A00*@MHS> To: Rust-mildew (Non Receipt Notification Requested) Subject: Conferences run by British Soc for Pl Path Sensitivity: Private You might like to know about two research conferences to be run by the British Society for Plant Pathology this year. The first is on "The Downy Mildew Fungi", to be held in Gwatt, Switzerland (!) from 29/8/95-2/9/95. Meeting organiser: Dr D.S. Shaw, School of Biological Sciences, University of Wales, Bangor, LL57 2UW, Wales. The second is on "The Gene-for-Gene Relationship: From Enigma to Exploitation", to be held at Warwick, England, from 12-15/12/95. Meeting organiser: Dr D.W. Parry, BSPP Programme Secretary, Harper Adams Agricultural College, Newport, Shropshire, TF10 8NB, England. The provisional programme for the downy mildew conference includes sessions on Systematics, Phylogeny and Diagnosis; Biology and Biochemistry; Genetical Variation; Host Resistance and the Genetics of Host-Pathogen Interactions; Techniques; Offered Papers; Chemical and Biological Control. The talks that I can identify as being about cereal downy mildews specifically are "Pathogenic and molecular variation in Sclerospora graminicola" (Thakur & Shaw) and "Marker-assisted selection of resistance to Sclerospora graminicola in pearl millet" (Witcombe & Hash). The draft programme for the gene-for-gene conference includes sessions on Genetic Analysis; Population Genetics; Gene Deployment and Breeding; Histological and Molecular Analyses; Molecular Genetics. It seems that around half the talks will concern cereal diseases. James Brown _______________________________________________________ Dr J. K. M. Brown, Cereals Research Department, John Innes Centre, Colney Lane, Norwich, NR4 7UH, England Phone: (+44)(0) 1603 452571. Fax: (+44)(0) 1603 502241. E-mail: james.brown@bbsrc.ac.uk From BIOSCI-REQUEST Sat Mar 18 17:59:16 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.10/8.6.6) id PAA28695 for rust-mil-list; Wed, 8 Mar 1995 15:44:25 -0800 Received: from abru.cg.com (abru.cg.com [199.98.86.3]) by net.bio.net (8.6.10/8.6.6) with SMTP id PAA28685 for ; Wed, 8 Mar 1995 15:44:18 -0800 Received: with SMTP-MR; Wed, 8 Mar 1995 23:41:06 EST MR-Received: by mta YOMAMA.MUAS; Relayed; Wed, 08 Mar 1995 23:41:06 -0500 (EST) MR-Received: by mta YOMAMA; Relayed; Wed, 08 Mar 1995 18:41:06 -0500 (EST) Disclose-recipients: prohibited Date: Wed, 8 Mar 1995 23:41:06 EST From: "John Salmeron (919) 541-8641" Subject: agars To: rust-mil@net.bio.net Message-id: <3506411808031995/A60393/YOMAMA/119344A90200*@MHS> Autoforwarded: false MIME-version: 1.0 Content-type: TEXT/PLAIN Importance: normal Priority: normal Sensitivity: Company-Confidential UA-content-id: 119344A90200 X400-MTS-identifier: [;3506411808031995/A60393/YOMAMA] Hop-count: 0 In the literature I have seen numerous references to "Oxoid agar #3" and "Czapek-Dox agar". Can anyone tell me where I can obtain the ingredients to prepare such media? Thank you. John Salmeron Senior Scientist Ciba Biotechnology Research From BIOSCI-REQUEST Mon Mar 20 00:22:10 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.11/8.6.6) id AAA03198 for rust-mil-list; Mon, 20 Mar 1995 00:22:10 -0800 Received: from caird.scri.sari.ac.uk (caird.scri.sari.ac.uk [134.36.144.10]) by net.bio.net (8.6.11/8.6.6) with ESMTP id AAA03190 for ; Mon, 20 Mar 1995 00:22:03 -0800 Received: from law.scri.sari.ac.uk (law.scri.sari.ac.uk [134.36.144.21]) by caird.scri.sari.ac.uk (8.6.9/8.6.9) with SMTP id IAA05039 for ; Mon, 20 Mar 1995 08:21:55 GMT Received: from LAW/MERCURY by law.scri.sari.ac.uk (Mercury 1.12); Mon, 20 Mar 95 8:22:30 GMT Received: from MERCURY by LAW (Mercury 1.12); Mon, 20 Mar 95 8:22:19 GMT From: "Adrian Newton" To: rust-mil@net.bio.net Date: Mon, 20 Mar 1995 08:22:17 GMT Subject: New web server for BSPP (British Society for Plant Pathology) Priority: normal X-mailer: Pegasus Mail/Windows (v1.22) Message-ID: <197B01A7FDD@law.scri.sari.ac.uk> A new web server has been set up at the Scottish Crop Research Institute for the British Society for Plant Pathology (BSPP). Its URL is: http://www.scri.sari.ac.uk/bspp Many of the link documents have yet to be written. Please be patient. Helpful comments to me (a.newton@scri.sari.ac.uk) please. Adrian Dr Adrian C Newton SCOTTISH CROP RESEARCH INSTITUTE Invergowrie, Dundee DD2 5DA, Scotland, UK Telephone: (UK) 01382 562731 : (International) +44 1382 562731 Fax: (UK) 01382 562426 : (International) +44 1382 562426 E-mail: a.newton@scri.sari.ac.uk From BIOSCI-REQUEST Mon Mar 20 03:06:54 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.11/8.6.6) id DAA08126 for rust-mil-list; Mon, 20 Mar 1995 03:06:54 -0800 Received: from sprintf.merit.edu (sprint.com [198.70.61.62]) by net.bio.net (8.6.11/8.6.6) with SMTP id DAA08121 for ; Mon, 20 Mar 1995 03:06:51 -0800 X400-Received: by mta merit in /PRMD=internet/ADMD=telemail/C=us/; Relayed; Mon, 20 Mar 1995 06:06:28 -0500 X400-Received: by /ADMD=TELEMAIL/C=US/; Relayed; Mon, 20 Mar 1995 06:01:04 -0500 X400-Received: by /PRMD=SMXFL2/ADMD=TELEMAIL/C=US/; Relayed; Mon, 20 Mar 1995 06:06:13 -0500 X400-Received: by /PRMD=LANGATE/ADMD=TELEMAIL/C=GB/; Relayed; Mon, 20 Mar 1995 06:05:00 -0500 Date: Mon, 20 Mar 1995 06:05:00 -0500 X400-Originator: Bill.Hollins@pbigb.sprint.com X400-Recipients: non-disclosure:; X400-MTS-Identifier: [/PRMD=LANGATE/ADMD=TELEMAIL/C=GB/;Mon Mar 20 06:05:58 199501] X400-Content-Type: P2-1984 (2) Content-Identifier: agars From: Bill.Hollins@pbigb.sprint.com Message-ID: <"Mon Mar 20 06:05:58 199501*/G=Bill/S=Hollins/OU=1321CACS/O=TMGB.PBI/PRMD=LANGATE/ADMD=TELEMAIL/C=GB/"@MHS> To: Rust-mil@net.bio.net Cc: salmeronj@am.abru.cg.com Subject: agars MIME-version: 1.0 Content-type: multipart/mixed; boundary="DGe2T1u0raSbWgrUjT6rQkqmOCPNOt06" --DGe2T1u0raSbWgrUjT6rQkqmOCPNOt06 Content-type: text/plain; charset="us-ascii" ---------------------------- Forwarded with Changes --------------------------- From: salmeronj@am.abru.cg.com at INTERNET Date: 3/18/95 9:26PM *To: rust-mil@net.bio.net at INTERNET cc: Peter Jack at 1321CACS cc: Bill Hollins at 1321CACS Subject: agars ------------------------------------------------------------------------------- --DGe2T1u0raSbWgrUjT6rQkqmOCPNOt06 RFC-822-Headers: MR-Received: by mta YOMAMA.MUAS; Relayed; Wed, 08 Mar 1995 23:41:06 -0500 (EST) MR-Received: by mta YOMAMA; Relayed; Wed, 08 Mar 1995 18:41:06 -0500 (EST) Disclose-recipients: prohibited Content-type: TEXT/PLAIN UA-content-id: 119344A90200 Hop-count: 0 --DGe2T1u0raSbWgrUjT6rQkqmOCPNOt06 Content-type: text/plain; charset="us-ascii" In the literature I have seen numerous references to "Oxoid agar #3" and "Czapek-Dox agar". Can anyone tell me where I can obtain the ingredients to prepare such media? Thank you. John Salmeron Senior Scientist Ciba Biotechnology Research In the UK from Unipath tel. 0256 841144 or Difco Laboratories tel. 081 9799951 they come in powder form - just add water! The Oxoid Company is now "Unipath" Bill Hollins Plant Breeding International Cambridge --DGe2T1u0raSbWgrUjT6rQkqmOCPNOt06--