From BIOSCI-REQUEST Tue Aug 1 04:44:31 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id EAA18452 for rust-mil-list; Tue, 1 Aug 1995 04:44:31 -0700 Received: from rishp1.risoe.dk (rishp1.risoe.dk [130.226.48.13]) by net.bio.net (8.6.12/8.6.6) with ESMTP id EAA18444 for ; Tue, 1 Aug 1995 04:44:17 -0700 Received: from risrms1.risoe.dk by rishp1.risoe.dk with ESMTP (1.37.109.16/16.2) id AA050777343; Tue, 1 Aug 1995 13:42:23 +0200 Received: from RISRMS1/SpoolDir by risrms1.risoe.dk (Mercury 1.21); 1 Aug 95 13:42:23 +0100 Received: from SpoolDir by RISRMS1 (Mercury 1.21); 1 Aug 95 13:42:08 +0100 From: "Hanne +stergRhord" Organization: Risoe National Laboratory, Denmark To: rust-mil@net.bio.net Date: Tue, 1 Aug 1995 13:42:03 MET Subject: COST 817, Short Term Scientific Missions Priority: normal X-Mailer: Pegasus Mail for Windows (v2.01) Message-Id: To participants in COST 817, This to remind you that the next deadline for applications for Short-Term Scientific Missions is September 15th 1995. The Application Form and Guidelines can be requested from the national delegates of the Management Committee, from Prof. Masson in Brussels or from myself. Note that the objectives of the mission should be to learn a new technique (broadly speaking), to make measurements or analyses of data using instruments or methods not available in your own lab, or to finish manuscripts. The duration should be between 3 days and 1 month and the maximum amount to be requested is 1500 ECU per mission. COST will pay travel and living expenses, but not research expenses. The application should say what the visit is intended to achieve, why a visit is necessary and why the visit should be to that particular laboratory, and should explain the relevance of the visit to the applicant's research programme and to the aims of the COST project. Only scientists from labs actively participating in the COST Action can apply. --------------------------- Hanne Oestergaard Environmental Science and Technology Department Risoe National Laboratory Post Box 49 DK-4000 Roskilde Denmark Direct phone: 45 4677 4111 --------------------------- From BIOSCI-REQUEST Wed Aug 2 18:09:47 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id SAA12484 for rust-mil-list; Wed, 2 Aug 1995 18:09:47 -0700 Received: from bos1h.delphi.com (SYSTEM@bos1h.delphi.com [192.80.63.8]) by net.bio.net (8.6.12/8.6.6) with ESMTP id SAA12476 for ; Wed, 2 Aug 1995 18:09:38 -0700 Received: from delphi.com by delphi.com (PMDF V4.3-9 #10880) id <01HTM129ZAR490NCIM@delphi.com>; Wed, 02 Aug 1995 21:07:47 -0400 (EDT) Date: Wed, 02 Aug 1995 21:07:47 -0400 (EDT) From: "SYAMA@DELPHI.COM" Subject: Repeated food poisoning episodes, help To: rust-mil@net.bio.net Message-id: <01HTM129ZKEA90NCIM@delphi.com> X-VMS-To: INTERNET"rust-mil@net.bio.net" MIME-version: 1.0 Content-type: TEXT/PLAIN; CHARSET=US-ASCII Content-transfer-encoding: 7BIT We are in need of assistance of determining the cause of repeated food poisoning episodes. Specifically, we are seeking the assistance of any doctors, chemists or microbiologists who might be able to shed some light on the cause and cure for our problem, and we are willing to hire an expert in this area. Any help would be much appreciated. The details are as follows: John has had food poisoning on three occasions recently with different food preparations, each time suffering the same symptoms. Others who ate those preparations also experienced the same or similar symptoms. These same foods have been eaten in the same combinations on other occasions without causing problems which would seem to rule out food allergies. We know which preparations are the cause of the problems because on each occasion, those were the only dishes consumed at the particular meal and others eating those same preparations suffered similarly. We had the foods analyzed at a chemical lab (see results below) without any conclusive results. We're wondering if there might be some chemical reaction with the utensils or something else we haven't considered. We are hoping you will be able to shed some light on the possible cause. The details of each incident are as follows: On May 30, the food consumed was "macaroni bake" (consisting of fresh button mushrooms, small elbow macaroni, broccoli, bell pepper, green onion, sesame oil, ascefatida, soy sauce, sweet basil, lemon juice, natural sea salt, bouillon cube flavoring, organic butter, organic whole milk, cheese and paprika), tofu and mata paneer (curd dish). The symptoms John experienced were flatulence and burping ("sulphur" burps) after one hour; abdominal cramps 6 hours later; vomitting and nausea afterwards lasting about 11 hours or more. Another person experienced flatulence, bloating, nausea but no vomitting and took a few days to fully recover. On June 20, the food consumed was oat water (consisting of strained cooked oats and purified water.) The symptoms John experienced was bloated stomach, flatulence and nausea. Another person experienced flatulence, bloating, headache and nausea for a few hours. A third person experienced bloating, flatulence and a little nausea which went away fairly quickly. On August 1, the food consumed was rice porridge (consisting of rice and purified water.) John ate the most of the three people who tried this dish. John added about 1/3 of an avocado (which was definitely not rotten) to the rice porridge while it was still relatively hot. The avocado had some sodium vitamin C (sodium ascorbate crystals) sprinkled on it. The symptoms John experienced was the same sulphur burps, followed later by gastroentiritis symptoms (nausea, vomitting) followed by diahorrhea. Two other people experienced mild symptoms of flatulence and a little bloating lasting around 45 minutes. They did not eat any of the avocado. All utensils used (spoons, knives, spatulas, etc.) are stainless steel. The pots are Revere Ware. A Corning Ware dish was used for the macaroni bake. The cutting boards are made of organic wood. Some of the cooking pots are old and pitted, however, heavy metal testing was done and doesn't seem to be the problem. The food is prepared in a home under strict aseptic conditions and transported to another home for consumption. The food is cooked, put into stainless steel fite pans after cooking, wrapped in towels and driven to the other home (about 30 minutes away). They are then put into the oven set at 158 degrees or refrigerate (depending on the preparation.) The kitchen the meals are prepared in is very clean, however, it tends to be steamy with no air flow--moisture collects on the window sills when cooking is going on. One of the food preparers has chronic psoriasis on his head and while he wears a hairnet, gloves and a germ mask while cooking, we wonder if flakes from his clothing may be causing a problem? Lab Results Date: June 1, 1995 Cheese Units Methods Aerobic Plate Count 35 deg. C 2.4E6 CFU/g pour Presumptive Coliforms <3 MPN/g mpn fd Faecal Coliforms Nil MPN/g mpn fd Coag positive Staphylococci <10 CFU/g spread Bacillus Cereus <10 CFU/g spread Clostridium perfringens <10 CFU/g spread Mushrooms Aerobic Plate Count 35 deg. C 1.7E6 CFU/g pour Presumptive Coliforms 1100 MPN/g mpn fd Faecal Coliforms <3 MPN/g mpn fd Coag positive Staphylococci <10 CFU/g spread Bacillus Cereus <10 CFU/g spread Clostridium perfringens <10 CFU/g spread Milk Aerobic Plate Count 35 deg. C 7.2E4 CFU/g pour Presumptive Coliforms <3 MPN/g mpn fd Faecal Coliforms Nil MPN/g mpn fd Coag positive Staphylococci <10 CFU/g spread Bacillus Cereus <10 CFU/g spread Clostridium perfringens <10 CFU/g spread Mata Paneer Aerobic Plate Count 35 deg. C - CFU/g pour Presumptive Coliforms - MPN/g mpn fd Faecal Coliforms - MPN/g mpn fd Coag positive Staphylococci - CFU/g spread Bacillus Cereus - CFU/g spread Clostridium perfringens Absent /g spd/en Clostridium perfringens - CFU/g spread Staphylococcal Enterotoxin - - immuno Tofu Aerobic Plate Count 35 deg. C 8.4E6 CFU/g pour Presumptive Coliforms >1100 MPN/g mpn fd Faecal Coliforms <3 MPN/g mpn fd Coag positive Staphylococci <10 CFU/g spread Bacillus Cereus <10 CFU/g spread Clostridium perfringens Absent /g spd/en Clostridium perfringens <10 CFU/g spread Staphylococcal Enterotoxin Absent - immuno Methods of Analysis: immuno By immunoassay mpn fd MPN Method for Foods pour Pour Plate Enumeration spd/en Spread Plate/Selective Enrich spread Spread Plate Enumeration Comments: Results apply to samples as received. Methods used are Public Health Laboratory Methodologies of the Institute of Environmental Science and Research (ESR). Coagulase positive Staphylococcus aureus was isolated from the mushrooms in low numbers. The only significant result from the foods tested was the Bacillus cereus count obtained from the organic milk. However, due to this product having exceeded the best before date, this result cannot be tied to the quality of the milk before it reached the best before date. It is recommended that products which have reached the best before date should not be consumed. It this product was consumed after the best before date, it is quite possible that it may have been the cause of the symptoms exhibited by the patients. It is not known whether the pasteurization process that the organic milk was put through was adequate. This could mean that Bacillus cereus organisms may have been present in the milk and were able to multiply to the levels found in the sample tested. Date: June 26, 1995 Item Yeast & Mould Staphylococcus Bacillus (c.f.u. per g) aureus (c.f.u. cereus per g) (c.f.u. per g) oat water batch <10 <10 <100 rolled oats batch <10 <10 <100 Uncle Tobys <10 <10 <100 traditional oats Methods used are those laid down by the Association of Official Analytical Chemists in the 7th Edition of the Bacteriological Analytical Manual 1992. The Compendium of Methods for the Microbiological Examination of Foods. 3rd Edition, 1992. From BIOSCI-REQUEST Thu Aug 3 14:00:48 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id OAA06093 for rust-mil-list; Thu, 3 Aug 1995 14:00:48 -0700 Received: from bos1g.delphi.com (SYSTEM@bos1g.delphi.com [192.80.63.7]) by net.bio.net (8.6.12/8.6.6) with ESMTP id OAA06089 for ; Thu, 3 Aug 1995 14:00:43 -0700 Received: from delphi.com by delphi.com (PMDF V4.3-9 #10880) id <01HTN6OVXCIU8WWVDF@delphi.com>; Thu, 03 Aug 1995 16:58:54 -0400 (EDT) Date: Thu, 03 Aug 1995 16:58:54 -0400 (EDT) From: "SYAMA@DELPHI.COM" Subject: repeated food poisoning episodes To: rust-mil@net.bio.net Message-id: <01HTN6OVXCIW8WWVDF@delphi.com> X-VMS-To: INTERNET"rust-mil@net.bio.net" MIME-version: 1.0 Content-type: TEXT/PLAIN; CHARSET=US-ASCII Content-transfer-encoding: 7BIT My humble apologies but there was a significant typo in the post I sent yesterday concerning the analyses of food in the repeated food poisoning episodes. The Bacillus cereus count in the milk was >5000 CFU/g (not <10 CFU/g as I posted yesterday.) The (revised) results are as follows: Lab Results Date: June 1, 1995 Cheese Units Methods Aerobic Plate Count 35 deg. C 2.4E6 CFU/g pour Presumptive Coliforms <3 MPN/g mpn fd Faecal Coliforms Nil MPN/g mpn fd Coag positive Staphylococci <10 CFU/g spread Bacillus Cereus <10 CFU/g spread Clostridium perfringens <10 CFU/g spread Mushrooms Aerobic Plate Count 35 deg. C 1.7E6 CFU/g pour Presumptive Coliforms 1100 MPN/g mpn fd Faecal Coliforms <3 MPN/g mpn fd Coag positive Staphylococci <10 CFU/g spread Bacillus Cereus <10 CFU/g spread Clostridium perfringens <10 CFU/g spread Milk Aerobic Plate Count 35 deg. C 7.2E4 CFU/g pour Presumptive Coliforms <3 MPN/g mpn fd Faecal Coliforms Nil MPN/g mpn fd Coag positive Staphylococci <10 CFU/g spread Bacillus Cereus >5000 CFU/g spread Clostridium perfringens <10 CFU/g spread Mata Paneer Aerobic Plate Count 35 deg. C - CFU/g pour Presumptive Coliforms - MPN/g mpn fd Faecal Coliforms - MPN/g mpn fd Coag positive Staphylococci - CFU/g spread Bacillus Cereus - CFU/g spread Clostridium perfringens Absent /g spd/en Clostridium perfringens - CFU/g spread Staphylococcal Enterotoxin - - immuno Tofu Aerobic Plate Count 35 deg. C 8.4E6 CFU/g pour Presumptive Coliforms >1100 MPN/g mpn fd Faecal Coliforms <3 MPN/g mpn fd Coag positive Staphylococci <10 CFU/g spread Bacillus Cereus <10 CFU/g spread Clostridium perfringens Absent /g spd/en Clostridium perfringens <10 CFU/g spread Staphylococcal Enterotoxin Absent - immuno Methods of Analysis: immuno By immunoassay mpn fd MPN Method for Foods pour Pour Plate Enumeration spd/en Spread Plate/Selective Enrich spread Spread Plate Enumeration Comments: Results apply to samples as received. Methods used are Public Health Laboratory Methodologies of the Institute of Environmental Science and Research (ESR). Coagulase positive Staphylococcus aureus was isolated from the mushrooms in low numbers. The only significant result from the foods tested was the Bacillus cereus count obtained from the organic milk. However, due to this product having exceeded the best before date, this result cannot be tied to the quality of the milk before it reached the best before date. It is recommended that products which have reached the best before date should not be consumed. It this product was consumed after the best before date, it is quite possible that it may have been the cause of the symptoms exhibited by the patients. It is not known whether the pasteurization process that the organic milk was put through was adequate. This could mean that Bacillus cereus organisms may have been present in the milk and were able to multiply to the levels found in the sample tested. Date: June 26, 1995 Item Yeast & Mould Staphylococcus Bacillus (c.f.u. per g) aureus (c.f.u. cereus per g) (c.f.u. per g) oat water batch <10 <10 <100 rolled oats batch <10 <10 <100 Uncle Tobys <10 <10 <100 traditional oats Methods used are those laid down by the Association of Official Analytical Chemists in the 7th Edition of the Bacteriological Analytical Manual 1992. The Compendium of Methods for the Microbiological Examination of Foods. 3rd Edition, 1992. From BIOSCI-REQUEST Mon Aug 7 02:16:03 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id CAA14344 for rust-mil-list; Mon, 7 Aug 1995 02:16:03 -0700 Received: from net2.wau.nl (NET2.WAU.NL [137.224.10.18]) by net.bio.net (8.6.12/8.6.6) with ESMTP id CAA14319 for ; Mon, 7 Aug 1995 02:15:54 -0700 Received: from vines2.wau.nl by NET2.WAU.NL (PMDF V4.3-10 #6821) id <01HTSFUEFE3K006KZP@NET2.WAU.NL>; Mon, 07 Aug 1995 11:14:53 +0000 (GMT) Received: by vines2.wau.nl; Mon, 7 Aug 95 11:16:53 +0100 Date: Mon, 07 Aug 1995 10:59:10 +0100 (CET) From: Rients.Niks@users.pv.wau.nl (rients niks) Subject: selective fungicides To: rust-mil@net.bio.net Message-id: X-Envelope-to: rust-mil@net.bio.net Content-transfer-encoding: 7BIT X-Priority: 3 (Normal) Dear Colleagues, I would like to thank you for all the suggestions for a fungicide that is effective against powdery mildew but not against rust. I recieved suggestions from 7 colleagues. Since we were looking for a replacement of MILGO-E, which is not available anymore, we think that especially Bupirimate Nimrod (ICI) is worth while investigation. This fungicide is, according to ICI, chemically related to MILGO-E (so, related to ethirimol), and is recommended against mildew on cucumber and tomato (where no rusts occur, and consequently a wide spectrum is not needed). We tried this in the field, and the results were promising, but because the crop was almost mature (end of season), we need more tests to check the lack of effect on rust. For application in the field we would need a special permission. Another chemical suggested, MILCURB, would be equally interesting (also related to ethirimol), but is also not available anymore. We will focus on NIMROD for the time being. When we feel more sure about its effectiveness and its selectivity, we will report again to rust-mil. Of course I remain interested in all further suggestions on the subject. Rients Niks From BIOSCI-REQUEST Wed Aug 9 23:29:28 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id XAA28192 for rust-mil-list; Wed, 9 Aug 1995 23:29:28 -0700 Received: from morgan.angis.su.OZ.AU (morgan.angis.su.OZ.AU [129.78.25.38]) by net.bio.net (8.6.12/8.6.6) with ESMTP id XAA26479 for ; Wed, 9 Aug 1995 23:27:13 -0700 Received: (from pbic00@localhost) by morgan.angis.su.OZ.AU (8.6.10/8.6.6) id QAA02581 for Rust-mil@net.bio.net; Thu, 10 Aug 1995 16:25:04 +1000 Date: Thu, 10 Aug 1995 16:25:04 +1000 From: "Plant Breeding Inst." Message-Id: <199508100625.QAA02581@morgan.angis.su.OZ.AU> To: Rust-mil@net.bio.net Content-Length: 1211 TO: Rust-mil@net.bio.net Rust and Mildew Workers The first confirmed report of stripe rust for the 1995 crop year in Australia was from Dr. F. Ellison at PBI Narrabri, northern New South Wales. Rust was found on spreader traps designed to attract natural inoculum. This is not the first time that initial reports have come from research stations where host materials with a wide range of responses are grown and where personnel are usually focussed to detect early incidence of diseases. It is of interest that 1994 was a year of extreme drought in the region. There were few wheat crops in northern N.S.W. and Queensland and not one sample of stripe rust was obtained. Only 11 samples came from southern N.S.W. Pathotyping of the current rust is yet to occur. If past experinece is borne out, we expect to find pt 104 E137 A- (A- = Avocet avirulent), the original introduction to Australia. The Narrabri sample is unlikely to be 110 E143 A+ (Avocet virulent) the main test pathotype used at Cobbitty, and hence the most likely candidate if dispersal from Cobbitty was involved. Our 1994 Cereal Rust Survey Annual Report is available on request. C.R. Wellings and R.A. McIntosh From BIOSCI-REQUEST Thu Aug 17 08:50:55 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id IAA23585 for rust-mil-list; Thu, 17 Aug 1995 08:50:55 -0700 Received: from mail.unet.umn.edu (mail.unet.umn.edu [128.101.101.103]) by net.bio.net (8.6.12/8.6.6) with SMTP id IAA23544; Thu, 17 Aug 1995 08:50:23 -0700 Received: from puccini.crl.umn.edu by mail.unet.umn.edu (5.65c) id AA18037; Thu, 17 Aug 1995 10:48:25 -0500 Message-Id: <199508171548.KAA03831@puccini.crl.umn.edu> Received: from [199.3.240.128] by puccini.crl.umn.edu; Thu, 17 Aug 1995 10:48:52 -0500 X-Sender: paulz@puccini.crl.umn.edu (Unverified) Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Thu, 17 Aug 1995 10:51:45 -0500 To: WIFDWC@trillium.uvm.edu, SOUTHPATH@trillium.uvm.edu, NEFPW@trillium.uvm.edu, FORPATH@trillium.uvm.edu, mycology@net.bio.net, rust-mil@net.bio.net From: paulz@puccini.crl.umn.edu (Paul Zambino) Subject: white pine blister rust Greetings forest pathologists and mycologists! I am currently studying molecular and pathogenic variation in white pine blister rust in North America and am trying to increase my representation of collections in many geographic regions. If any of you would be interested in collecting a few rust-infected Ribes leaves this fall, I will greatly appreciate your help. Let me know, and I will send collection envelopes and dessicant to keep the urediniospores viable so that I will be able to increase and purify the strains. I would also be interested in knowing of European and Asian contacts that could send me non-viable spores or extracted DNA. Thanks! Paul Zambino USDA Forest Service Forestry Sciences Lab 5985 Highway K Rhinelander, WI 54501 PH:(715)362-1178 FAX: (715)362-1166 EMAIL: paulz@puccini.crl.umn.edu DG:p.zambino:R09F06A From BIOSCI-REQUEST Thu Aug 24 14:22:53 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id OAA16761 for rust-mil-list; Thu, 24 Aug 1995 14:22:53 -0700 Received: from mailhost.ksu.ksu.edu (root@grunt.ksu.ksu.edu [129.130.12.17]) by net.bio.net (8.6.12/8.6.6) with ESMTP id OAA16758 for ; Thu, 24 Aug 1995 14:22:51 -0700 Received: from plantpath.pp.ksu.edu ([129.130.90.39]) by mailhost.ksu.ksu.edu (8.6.12) with ESMTP id QAA00056 for ; Thu, 24 Aug 1995 16:20:51 -0500 Received: from PLANTPATH/MERCQUEUE by plantpath.pp.ksu.edu (Mercury 1.21); 24 Aug 95 16:20:46 +600 Received: from MERCQUEUE by PLANTPATH (Mercury 1.21); 24 Aug 95 16:20:14 +600 From: "Kurt Zeller" Organization: Plant Pathology To: rust-mil@net.bio.net Date: Thu, 24 Aug 1995 16:20:13 CST6CDT Subject: subscribe Priority: normal X-mailer: Pegasus Mail for Windows (v2.01) Message-ID: <1D7FC82AC5@plantpath.pp.ksu.edu> subscribe kzeller@plantpath.pp.ksu.edu From BIOSCI-REQUEST Thu Aug 31 18:22:25 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id SAA08067 for rust-mil-list; Thu, 31 Aug 1995 18:22:25 -0700 Received: from morgan.angis.su.OZ.AU (morgan.angis.su.OZ.AU [129.78.25.38]) by net.bio.net (8.6.12/8.6.6) with ESMTP id SAA08063 for ; Thu, 31 Aug 1995 18:22:20 -0700 Received: (from pbic00@localhost) by morgan.angis.su.OZ.AU (8.6.10/8.6.6) id LAA09332 for Rust-mil@net.bio.net; Fri, 1 Sep 1995 11:20:06 +1000 Date: Fri, 1 Sep 1995 11:20:06 +1000 From: "Plant Breeding Inst." Message-Id: <199509010120.LAA09332@morgan.angis.su.OZ.AU> To: Rust-mil@net.bio.net Subject: A new gene for Resistance to Stripe Rust Content-Length: 1310 I propose to name a new gene for resistance to stripe rust as Yr24. This gene came to me in a segregating backcross population derived from a synthetic wheat involving a durum (K733) and Aegilops squarrosa. Yr24 is readily recognised in the durum parent. Low infection type varies from ;NN to 1NN. Yr24 is located in chromosome 1B and is about 5cm from Yr15 and based on this level of recombination it should be in 1BS and should not be allelic with Yr10. Chen et al. (1995) located and designated gene Yr21 (=YrLemhi) in chromosome 1B but linkage relationships are unknown. As Australian isolates of P.striiformis are virulent on seedlings of Lemhi, we are unable to show linkage relationships between Yr24 and Yr21. Yr24 confers resistance to all Australian pathotypes. The reaction is illustrated in Plate 4-26 in McIntosh, Wellings and Park 1995. Wheat Rusts: An Atlas of Resistance Genes. Proposed gene catalogue listing will be: Yr24 (9633). 1BS (9633) V:Meering*3/ /K733/Aeg.squarrosa. 9633. McIntosh R.A. et al. 1995. In preparation. In addition to the bionet this communication will be sent to the following. Please advise colleagues. Comments by 30 September 1995. C.R. Wellings M. Cromey R.F. Line S. Nayar R. Johnson M. Aslam K. Leonard O. Mamluk Bob McIntosh From BIOSCI-REQUEST Thu Aug 31 18:34:49 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id SAA08522 for rust-mil-list; Thu, 31 Aug 1995 18:34:49 -0700 Received: from morgan.angis.su.OZ.AU (morgan.angis.su.OZ.AU [129.78.25.38]) by net.bio.net (8.6.12/8.6.6) with ESMTP id SAA08516 for ; Thu, 31 Aug 1995 18:34:42 -0700 Received: (from pbic00@localhost) by morgan.angis.su.OZ.AU (8.6.10/8.6.6) id LAA11328 for Rust-mil@net.bio.net; Fri, 1 Sep 1995 11:32:22 +1000 Date: Fri, 1 Sep 1995 11:32:22 +1000 From: "Plant Breeding Inst." Message-Id: <199509010132.LAA11328@morgan.angis.su.OZ.AU> To: Rust-mil@net.bio.net Subject: Designation of Suppressors of Genes for Disease Resistance. Content-Length: 761 Dear Colleagues, With the increasing number of reports on the genetic suppression of resistance conferred by characterised genes for disease resistance, it is time to address the problem of genetic nomenclature of such suppressors. It appears that suppressors are specific in effect. Evidence for suppression of resistance comes largely from wide crosses in that resistance phenotypes of one species may be lost in crosses of hybrids or amphiploids with a second species. A common phenomenon with hexaploid wheat is the reduced level of restance (partial suppression?) as genes are moved from lower to higher levels of ploidy. There are two instances in wheat where suppressors of resistance could/should be designated ATDT02,3518989 kjjjATDT02,3518989 From BIOSCI-REQUEST Thu Aug 31 19:41:17 1995 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id TAA12181 for rust-mil-list; Thu, 31 Aug 1995 19:41:17 -0700 Received: from morgan.angis.su.OZ.AU (morgan.angis.su.OZ.AU [129.78.25.38]) by net.bio.net (8.6.12/8.6.6) with ESMTP id TAA12174 for ; Thu, 31 Aug 1995 19:41:09 -0700 Received: (from pbic00@localhost) by morgan.angis.su.OZ.AU (8.6.10/8.6.6) id MAA22901 for Rust-mil@net.bio.net; Fri, 1 Sep 1995 12:39:04 +1000 Date: Fri, 1 Sep 1995 12:39:04 +1000 From: "Plant Breeding Inst." Message-Id: <199509010239.MAA22901@morgan.angis.su.OZ.AU> To: Rust-mil@net.bio.net Subject: Designation of Suppressors of Genes for Disease Resistance Content-Length: 3741 Dear Colleagues, With the increasing numbers of reports on the genetic suppression of resistance conferred by characterised genes for disease resistance, it is time to address the problem of genetic nomenclature of such suppressors. It appears that suppressors are specific in effect. Evidence for suppression of resistance comes largely from wide crosses in that resistance phenotypes of one species may be lost in crosses of hybrids or amphiploids with a second species. A common phenomenon with a hexaploid wheat is the reduced level of resistance (partial suppression?) as genes are moved from lower to higher levels of ploidy. There are two instances in wheat where suppressors of resistance could/should be designated currently. The first, discovered by Kerber and co-workers at Winnipeg, involves a dominant gene in chromosome 7D that reduces the expression of stem rust resistance conferred by gene(s) in the A and/or B genome(s). Workers at Winnipeg and Fargo have used knowledge of this behaviour to induce mutants of the suppressor gene thus permitting bread wheat to express a phenotype obtained in nulli-7D plants or in durum. At this stage, the identity of the gene(s) that are suppressed is unknown - my personal opinion is that Sr12 is important. It may be of interest that I have been unable to repeat these differences using Australian P.graminis isolates. The best I get is ; versus 0; with avirulent cultures for Canthatch and desuppressed mutant lines from Fargo; otherwise both are IT 3+. The second is from this laboratory and involves suppression of Pm8 located in the 1BL.1RS translocation chromosome. The dominant suppressor of Pm8 is located in chromosome 1A. Suppression appears to be specific for Pm8. The rules of genetic nomenclature allows for the use of I or Su for dominant inhibitors or suppressors. So using the Pm8 example the suppressor could be designated IPm8 or SuPm8 (or Ipm8 or Supm8). We cannot use I-Pm8 and Su-Pm8 because the - conveys a different meaning. Su is already used as a stand-alone symbol in wheat and I is already combined with small letters in, for example, Ibf and Igc. Since we tend to be calling the genes "suppressors" rather than "inhibitors", I prefer the symbol SuPm8 - the capital P provides the unique part of the symbol as Supm could be an abbreviation for a totally different trait. I need guidance in reaching a consensus for cataloguing suppressors of genes for disease resistance. I suggest we need to know the identity of the resistance gene (or at least its source) and its location, and we need to know the specificity and location of the suppressor. If there is agreement on this then I propose the following addition to special rules for Pathogenic Disease/Pest Reaction for the Catalogue of Gene Symbols for Wheat. Specific suppressors of resistance conferred by genes for disease reaction will be designated by Su followed by the name of the gene that is suppressed. Such suppressors will be recognised in gene lists because genes for disease/ pest reaction are always named with an upper case letter. Proposed listing for Catalogue of Gene Symbols for Wheat: Pm8 Suppressor of Pm8 SuPm8 (9632) 1AS (9632) V: Many wheats with the 1BL.1RS translocation, especially frequent in populations from CIMMYT. 9632. Ren, McIntosh, Sharp & The. 1995. In preparation. Please provide comments by 30 September, 1995. In addition to the Rust Mildew Bulletin Board, this communication will be sent to Dr. G. Hart Texas A & M University Dr. M. Gale John Innes Centre Dr. N. Williams N D State University Dr. E. Kerber Agriculture Canada, Winnipeg Please bring this matter to the attention of colleagues. Bob Mc Intosh