From BIOSCI-REQUEST Mon Nov 4 04:53:27 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id EAA17300; Mon, 4 Nov 1996 04:53:27 -0800 Received: from danpost.uni-c.dk (danpost.uni-c.dk [129.142.6.64]) by net.bio.net (8.6.12/8.6.6) with ESMTP id EAA17284; Mon, 4 Nov 1996 04:52:47 -0800 Received: from gate.mem.dk (gate.mem.dk [130.226.166.132]) by danpost.uni-c.dk (8.7.5/8.6) with SMTP id NAA16090 for ; Mon, 4 Nov 1996 13:53:12 +0100 (MET) Received: by gate.mem.dk (AIX 4.1/UCB 5.64/4.03) id AA20202; Mon, 4 Nov 1996 13:47:48 +0100 Received: from unknown(172.26.192.12) by gate via smap (V1.3) id sma018378; Mon Nov 4 13:47:20 1996 Received: by dmusn2.dmu.dk with SMTP (Microsoft Exchange Server Internet Mail Connector Version 4.0.993.5) id <01BBCA57.8F432B50@dmusn2.dmu.dk>; Mon, 4 Nov 1996 13:53:29 +0100 Message-Id: From: "Damgaard, Christian F." To: "'rust-mil@net.bio.net'" Subject: Second pathogen species Date: Mon, 4 Nov 1996 13:53:27 +0100 X-Mailer: Microsoft Exchange Server Internet Mail Connector Version 4.0.993.5 Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Content-Transfer-Encoding: 7bit Dear colleges I am interested in knowing of examples and getting references to the following question. Does an infection of one plant pathogen species increases the chance of the same plant being infected by another plant pathogen species ? Thankyou Christian Damgaard cfd@dmu.dk Christian Damgaard Department of Terrestrial Ecology National Environmental Research Institute Vejlsovej 25 8600 Silkeborg Denmark fax: +45-89201414 phone: +45-89201400 e-mail: cfd@dmu.dk From BIOSCI-REQUEST Mon Nov 4 08:56:36 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id IAA13251; Mon, 4 Nov 1996 08:56:36 -0800 Received: from unix1.sncc.lsu.edu (unix1.sncc.lsu.edu [130.39.129.86]) by net.bio.net (8.6.12/8.6.6) with SMTP id IAA13248; Mon, 4 Nov 1996 08:56:19 -0800 Received: by unix1.sncc.lsu.edu (AIX 3.2/UCB 5.64/4.03) id AA156480; Mon, 4 Nov 1996 10:58:37 -0600 Date: Mon, 4 Nov 1996 10:58:35 -0600 (CST) From: Eric Robb Siegel To: "Damgaard, Christian F." Cc: "'rust-mil@net.bio.net'" Subject: Re: Second pathogen species In-Reply-To: Message-Id: Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII On Mon, 4 Nov 1996, Damgaard, Christian F. wrote: > I am interested in knowing of examples and getting references to the > following question. > > Does an infection of one plant pathogen species increases the chance of > the same plant being infected by another plant pathogen species ? My understanding is that root-knot nematode infection of tomato plants normally resistant to Fusarium Wilt will make them susceptible. And Sudden Death Syndrome in Soybean, caused by *Fusarium solani*, is made much worse by co-infection with soybean cyst nematode. Eric Siegel From BIOSCI-REQUEST Mon Nov 4 23:38:54 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id XAA24148; Mon, 4 Nov 1996 23:38:54 -0800 Received: from inra.inra.fr (inra.inra.fr [138.102.88.1]) by net.bio.net (8.6.12/8.6.6) with ESMTP id XAA24143; Mon, 4 Nov 1996 23:38:37 -0800 Received: from ecgn.grignon.inra.fr (grignon.inra.fr [192.93.70.1]) by inra.inra.fr (8.7.6/8.7.3) with SMTP id IAA06652 for ; Tue, 5 Nov 1996 08:38:11 +0100 (MET) Received: from [192.93.70.186] (pathov3) by ecgn.grignon.inra.fr, Tue, 5 Nov 96 08:36:10 +0100 Date: Tue, 5 Nov 96 08:36:10 +0100 Message-Id: Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" X-Mailer: Eudora F1.5.4 To: rust-mil@net.bio.net From: sache@grignon.inra.fr (Ivan Sache) Subject: Re: Second pathogen species Christian asked: >I am interested in knowing of examples and getting references to the >following question. > >Does an infection of one plant pathogen species increases the chance of >the same plant being infected by another plant pathogen species ? A recent review of 'mycopredisposition', including several examples and examples, can be found in: Shaw, D.E. (1994) Mycopredisposition to susceptibility and the fungi involved. Australasian Plant Pathology 23:170-189. Hope it helps. Ivan Sache Laboratoire de Pathologie Vegetale INRA 78850 Thiverval-Grignon France ----------------------------------------------------- Phone : +33.01.30.81.54.35 Fax : +33.01.30.81.53.06 Mail : sache@grignon.inra.fr ----------------------------------------------------- From BIOSCI-REQUEST Wed Nov 6 01:47:20 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id BAA01750; Wed, 6 Nov 1996 01:47:20 -0800 Received: from arcs.cc.bbsrc.ac.uk (arcs.cc.bbsrc.ac.uk [149.155.100.5]) by net.bio.net (8.6.12/8.6.6) with ESMTP id BAA01617; Wed, 6 Nov 1996 01:46:40 -0800 Received: from pserv.jic.bbsrc.ac.uk by arcs.cc.bbsrc.ac.uk with SMTP (PP); Wed, 6 Nov 1996 09:48:49 +0000 Received: from mserv.jic.bbsrc.ac.uk by pserv.jic.bbsrc.ac.uk; (5.65/1.1.8.2/05Dec95-1142AM) id AA26243; Wed, 6 Nov 1996 09:47:11 GMT Received: by mserv.jic.bbsrc.ac.uk; (5.65/1.1.8.2/30Jan96-0405PM) id AA18392; Wed, 6 Nov 1996 09:49:10 GMT Alternate-Recipient: allowed Auto-Forwarded: prohibited Content-Return: allowed Disclose-Recipients: prohibited Conversion: allowed Importance: normal Priority: normal Sensitivity: Company-Confidential Subject: Studentship: evolution of Erysiphe graminis From: jbrown To: rust-mil@net.bio.net Message-Id: <961106094909.1734@mserv.jic.bbsrc.ac.uk.0> Date: Wed, 6 Nov 96 09:49:09 +0000 X-Mailer: MAILworks 1.7-A I have a Ph.D. studentship available in the area of molecular evolution of Erysiphe graminis. Please would you bring it to the attention of those who might be interested in doing a Ph.D. in this area? Many thanks, James Brown _______________________________________________________ Dr J. K. M. Brown, Cereals Research Department, John Innes Centre, Colney Lane, Norwich, NR4 7UH, England Phone: (+44)(0) 1603 452571. Fax: (+44)(0) 1603 502241. E-mail: james.brown@bbsrc.ac.uk _______________________________________________________ Postgraduate (Ph.D.) Research Studentship Molecular Evolution of Host Specificity in Cereal Powdery Mildews John Innes Centre, Norwich This studentship is for the study of the evolution of the fungus Erysiphe graminis, which causes powdery mildew, an economically important disease of cereals and grasses. Two aspects of the evolution of E. graminis are of interest. The first is the relationship between the "special forms" of E. graminis that infect different genera of cereals. This is of interest for two reasons. Firstly, the forms are usually thought to be taxonomically distinct, although they can be crossed in the laboratory. If they are inter-fertile in nature, there could be gene flow between them, so that virulence genes could move between the mildew fungi that infect different cereals. Secondly, it is often thought that parasite evolution should reflect the evolution of their hosts. This could be tested by comparing the phylogenies of the different forms of E. graminis and of the grasses which they infect. These questions could be studied by various methods, including population genetic analysis of variation in molecular markers, such as AFLP, comparison of the genetic maps of different forms of E. graminis and analysis of the distribution of repeated sequences in the genome. The second aspect is the relationship of E. graminis to other species of Erysiphe. The infection structures of E. graminis differ in several ways from those of other Erysiphe species, which infect dicots, and may be specialised for infection of cereals. We would like to know if E. graminis could have evolved from the dicot-infecting Erysiphe species. This can be studied by estimating a phylogeny from variation in the DNA sequences of one or more genes in a range of Erysiphe species. The student will be supervised by Dr James Brown, who runs the powdery mildew research group and specialises in population genetics of plant-pathogen interactions, and Professor Dick Flavell, who specialises in the organisation of the genomes of plants and fungi. The JIC is an internationally recognised centre of excellence for research in plant and microbial sciences, and has particular strengths in genetics and molecular biology. The student will be working in a well-equipped Cereal Pathology laboratory, which currently has around 20 staff and students, and will be registered with the University of East Anglia. We are interested in hearing from students who have, or expect to get, an upper second class degree. Applicants should be interested in applying molecular genetic methods to systematics and population genetics. An interest in mycology or plant pathology would also be desirable. This is a Special Studentship, funded by the Agricultural Systems Directorate of the BBSRC, and as such, will attract a substantially higher stipend than the usual rate for BBSRC grants. Applications, including a CV and the names and addresses of two academic referees, should be sent to the JIC's Ph.D. studentship coordinator, Mrs Anne Williams, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH. You are also welcome to contact James Brown to discuss this research project further (address as above, phone: 01603-452571, fax: 01603-502241, e-mail: James.Brown@bbsrc.ac.uk). From BIOSCI-REQUEST Wed Nov 6 23:39:43 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id XAA19167; Wed, 6 Nov 1996 23:39:43 -0800 Received: from dfw-ix11.ix.netcom.com (dfw-ix11.ix.netcom.com [206.214.98.11]) by net.bio.net (8.6.12/8.6.6) with ESMTP id XAA19156; Wed, 6 Nov 1996 23:39:27 -0800 Received: from 204.30.69.130 (min-mn4-02.ix.netcom.com [204.30.69.130]) by dfw-ix11.ix.netcom.com (8.6.13/8.6.12) with SMTP id XAA03949 for ; Wed, 6 Nov 1996 23:39:31 -0800 Message-ID: <328192CB.6D71@ix.netcom.com> Date: Thu, 07 Nov 1996 01:42:03 -0600 From: Michael Ravnitzky Reply-To: MikeRav@ix.netcom.com X-Mailer: Mozilla 2.01E (Macintosh; U; PPC) MIME-Version: 1.0 To: rust-mil@net.bio.net Subject: Get a list of hundreds of military tech reports on wheat and corn rusts, rye rusts, rice blast, crop mildew, anti-crop warfare, fungal diseases etc. X-URL: http://www.bio.net/hypermail/RUST-MILDEW/ Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit I thought you might find this an interesting government information resource, on subject[s] you or your associates are interested in. If not, I apologize. Could you forward it on to the right person? At a government agency called DTIC: Defense Technical Information Center, there are a large number of government technical reports on almost any subject under the sun. NOTE: Donıt bother using the DTIC web site--in a nutshell, itıs worthless and omits the bulk of the two million technical reports in the DTIC collection. Send a letter--youıll get much better results. You'll have to select your own keywords for the exact subjects youıre interested in. I suggest you select no more than ten keywords or less for each letter. Please select your keyword[s] carefully and include plural[s] like I just did. Put an *OR* between each keyword or subject term. The fee is likely to be free or only a few bucks. You probably want to include a statement in the letter such as *I agree to pay reasonable fees associated with this request. Please notify me if the cost will exceed $30.*, so that they won't delay the processing of the request. Remember, they will try to dissuade you from asking for such a list. If they send you a letter, and you donıt respond, they will withdraw your request and you wonıt get your information. To get your bibliography [list of technical reports], send a letter of request as follows: To: Defense Technical Information Center Attn: DTIC-RSM [Kelly D. Akers, FOIA Manager] 8725 John J. Kingman Road, Suite 0944 Fort Belvoir, VA 22060-6128 USA Phone: 703-767-9194 Dear Ms. Akers: I request the following records under the provisions of the Freedom of Information Act: A computer generated technical report bibliography [for all computerized index years] of reports on the subject[s]/keyword[s] of: ________________ OR _________________ OR ________________ OR _______________ OR _________________ OR _______________ This is a request for DTIC records, please don't forward my request to NTIS. Please include both classified and unclassified records in your search. If any of the records are classified, please review them for release, or the release of nonsensitive portions. I am an individual, noncommercial requester and this request is not being made for commercial purposes. [OR YOU MIGHT INSTEAD INDICATE IF YOU ARE A COMMERCIAL REQUESTER, OR AN EDUCATIONAL OR SCIENTIFIC INSTITUTION, OR A REPRESENTATIVE OF THE MEDIA] I also agree to pay up to $35 for reasonable fees associated with this request. Sincerely, ______________ From BIOSCI-REQUEST Fri Nov 8 08:16:59 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id IAA09877; Fri, 8 Nov 1996 08:16:59 -0800 Received: from arcs.cc.bbsrc.ac.uk (arcs.cc.bbsrc.ac.uk [149.155.100.5]) by net.bio.net (8.6.12/8.6.6) with ESMTP id IAA09842; Fri, 8 Nov 1996 08:16:40 -0800 Received: from pserv.jic.bbsrc.ac.uk by arcs.cc.bbsrc.ac.uk with SMTP (PP); Fri, 8 Nov 1996 16:19:09 +0000 Received: from mserv.jic.bbsrc.ac.uk by pserv.jic.bbsrc.ac.uk; (5.65/1.1.8.2/05Dec95-1142AM) id AA00472; Fri, 8 Nov 1996 16:17:25 GMT Received: by mserv.jic.bbsrc.ac.uk; (5.65/1.1.8.2/30Jan96-0405PM) id AA22751; Fri, 8 Nov 1996 16:19:28 GMT Alternate-Recipient: allowed Auto-Forwarded: prohibited Content-Return: allowed Disclose-Recipients: prohibited Conversion: allowed Importance: normal Priority: normal Sensitivity: Company-Confidential Subject: Post-doc From: James Brown To: rust-mil@net.bio.net, grains@greengenes.cit.cornell.edu Message-Id: <961108161926.1734@mserv.jic.bbsrc.ac.uk.0> Date: Fri, 8 Nov 96 16:19:27 +0000 X-Mailer: MAILworks 1.7-A I will shortly have a post-doc position available, for work on the genetics of Septoria tritici resistance in wheat. The following is the text of an advert that will be in Nature, probably on 14/11/96, otherwise on 21/11/96. Please bring it to the attention of any aspiring post-docs. (Apologies for cross-posting.) James Brown JOHN INNES CENTRE Norwich PLANT PATHOLOGIST Applications are invited for the post of Plant Pathologist in the Cereals Research Department, to research the genetics of the resistance of wheat to Septoria tritici. The project involves the use of molecular mapping to locate resistance genes controlling general resistance to S. tritici or interactions with specific isolate of the pathogen. The department has a well-established, multi-disciplinary pathology group, which researches a range of important diseases of wheat and barley, and has excellent facilities for genetic analysis of cereals. Further details of the position can be obtained from Dr James K.M. Brown (address given below, fax: +44 1603 502241, e-mail: James.Brown@bbsrc.ac.uk). Candidates should have a Ph.D. or equivalent experience in plant pathology, with further experience in genetics or molecular biology. The position will be on the Band 6 scale and the salary on appointment will be in the range #13,952 to #19,425 p.a. Funding is provided by the European Union and the appointment will be for a fixed term of three years. A non-contributory superannuation scheme is in operation. The John Innes Centre is an equal opportunities employer. Applications must be made on a form which can be obtained by writing to the Personnel Officer, John Innes Centre, Colney Lane, Norwich, NR4 7UH, fax: +44 1603 456844, e-mail: Sharon.Butler@bbsrc.ac.uk, quoting reference CR/154. The closing date for completed applications is 12th December 1996 (NB the closing date will be 19th Dec if the advert appears in the 21/11/96 issue of Nature, rather than the 14/11/96 issue). The John Innes Centre is associated with the Biotechnology and Biological Sciences Research Council Further information is available on the WWW: http://www.uea.ac.uk/nrp/jic/cjobs.htm From BIOSCI-REQUEST Sun Nov 10 02:00:26 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id CAA06253; Sun, 10 Nov 1996 02:00:26 -0800 Received: (from biohelp@localhost) by net.bio.net (8.6.12/8.6.6) id CAA06222; Sun, 10 Nov 1996 02:00:12 -0800 Date: Sun, 10 Nov 1996 02:00:12 -0800 From: BIOSCI Administrator Message-Id: <199611101000.CAA06222@net.bio.net> To: rust-mil@net.bio.net Subject: BIOSCI/bionet miniFAQ & Fundraiser (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From BIOSCI-REQUEST Tue Nov 12 00:39:00 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id AAA11594; Tue, 12 Nov 1996 00:39:00 -0800 Received: from relay-6.mail.demon.net (punt2.demon.co.uk [194.217.242.5]) by net.bio.net (8.6.12/8.6.6) with SMTP id AAA11584; Tue, 12 Nov 1996 00:38:43 -0800 Received: from scottp.demon.co.uk ([158.152.119.68]) by relay-5.mail.demon.net id ab518434; 12 Nov 96 8:33 GMT Message-ID: Date: Tue, 12 Nov 1996 08:25:16 +0000 To: rust-mil@net.bio.net From: Peter Scott Subject: Biology and IT MIME-Version: 1.0 X-Mailer: Turnpike Version 3.00 Members of this Newsgroup may be interested in a novel Conference organized by the British Society for Plant Pathology and the Systematics Association. (Please excuse some limited cross-posting.) In view of the expected appeal of this meeting to a wide range of biologists and information technologists, the following advertisement has been placed in Nature. It's not too late to register, even though the deadline is around now. It's not expensive. The Open Forum is free to all. Check out the Web page, in which we are providing lots of links as an experiment in the use of the Web to enhance the Conference. ----- CONFERENCE University of Kent, Canterbury, UK 16-19 December 1996 UNLOCKING THE FUTURE For everyone interested in the interface of BIOLOGY and INFORMATION TECHNOLOGY Special subjects - Information Technology in Plant Pathology - Computer-Based Species Identification Open Forum on - BIOLOGY & IT: THE ROAD AHEAD with Professor Sir Robert May Chief Scientific Advisor, UK Office of Science & Technology Professor Peter Cochrane Head of Advanced Applications & Technology, British Telecom Labs Details and Registration http://www.bspp.org.uk/dec96con.htm Dr Peter Jeffries, Research School of Biosciences, University of Kent, Canterbury CT2 7NZ, UK E-mail: p.jeffries@ukc.ac.uk. Tel: +44 (0)1227 764000 ext. 3478, Fax: +44 (0)1227 787465 ----- The Open Forum will be of special interest. You may well know both of the distinguished speakers from radio or television. To be sure of your place at the Conference, Members of BSPP and the Systematics Association are advised to book early. For a programme and booking form, visit the Web site or contact Peter Jeffries (see above). Please say whether you want to attend the Practical and Tutorial Session on 16 December ("Everything you always wanted to know about IT but were afraid to ask"), indicating your preferred topics. Offers of contributions welcome - see above. Peter -- Peter Scott Director, Information Institute, CAB INTERNATIONAL, Wallingford OX10 8DE, UK Tel: +44 1491 832111. Fax: +44 1491 833508 President, British Society for Plant Pathology Work: p.scott@cabi.org http://www.cabi.org Home: peter@scottp.demon.co.uk BSPP: http://www.bspp.org.uk ********************************************************************* Information Technology in Plant Pathology and Systematics BSPP/SA Meeting, University of Kent, 16-19 December 1996 SEE INTERACTIVE PROGRAMME AT http://www.bspp.org.uk/dec96con.htm ********************************************************************* From BIOSCI-REQUEST Mon Nov 18 06:25:45 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id GAA11577; Mon, 18 Nov 1996 06:25:45 -0800 Received: from arcs.cc.bbsrc.ac.uk (arcs.cc.bbsrc.ac.uk [149.155.100.5]) by net.bio.net (8.6.12/8.6.6) with ESMTP id GAA11570; Mon, 18 Nov 1996 06:25:24 -0800 Received: from pserv.jic.bbsrc.ac.uk by arcs.cc.bbsrc.ac.uk with SMTP (PP); Mon, 18 Nov 1996 14:28:29 +0000 Received: from mserv.jic.bbsrc.ac.uk by pserv.jic.bbsrc.ac.uk; (5.65/1.1.8.2/05Dec95-1142AM) id AA10002; Mon, 18 Nov 1996 14:26:44 GMT Received: by mserv.jic.bbsrc.ac.uk; (5.65/1.1.8.2/30Jan96-0405PM) id AA15231; Mon, 18 Nov 1996 14:29:03 GMT Alternate-Recipient: allowed Auto-Forwarded: prohibited Content-Return: allowed Disclose-Recipients: prohibited Conversion: allowed Importance: normal Priority: normal Sensitivity: Company-Confidential Subject: Ml(CP) From: James Brown To: rust-mil@net.bio.net Message-Id: <961118142903.32445@mserv.jic.bbsrc.ac.uk.0> Date: Mon, 18 Nov 96 14:29:03 +0000 X-Mailer: MAILworks 1.7-A Does anyone out there have a barley line which has Ml(CP) but not Mlg, and would be willing to let me have some seed of it? James Brown From BIOSCI-REQUEST Thu Nov 21 08:34:48 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id IAA07158; Thu, 21 Nov 1996 08:34:48 -0800 Received: from plains.nodak.edu (kalavach@plains.NoDak.edu [134.129.111.64]) by net.bio.net (8.6.12/8.6.6) with ESMTP id IAA07150; Thu, 21 Nov 1996 08:34:31 -0800 Received: (from kalavach@localhost) by plains.nodak.edu (8.8.3/8.8.3) id KAA06096; Thu, 21 Nov 1996 10:35:01 -0600 (CST) Date: Thu, 21 Nov 1996 10:34:57 -0600 (CST) From: Venugopal S Kalavacharla To: rust-mil@net.bio.net Subject: quantification-bean rust inoculum Message-ID: MIME-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII dear rust-mil netters, although i know that this newsgroup is devoted to rust and mildew of cereals, i am posting this mail as i am working with rust (Uromyces appendiculatus) of common bean. Problem: i would like to quantify my inoculum so that i know the amount of urediniospores that go into each inoculation, and then standardize the procedure. currently for bean rust, i have seen that the urediniospores are weighed (mg/ml), suspended in distilled water containing tween 20. but the problem is that the urediniospores are black in color making it difficult to discern between the spores and dirt (even after filtering through a sieve). Can we quantify spores by using something like (a large (?) haemocytometer) as is used for tan spot or white rust. Is there a better way, that is used for cereals that i can adapt for bean rust. also is there a way to collect spores instead of scraping them of the leaves, which is very messy and leading to loss of the spores. any pointers or references would be helpful thanks, Kal kalavach@plains.nodak.edu dept. of plant science ndsu, fargo, nd From BIOSCI-REQUEST Mon Nov 25 15:35:18 1996 Return-Path: BIOSCI-REQUEST Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id PAA19090; Mon, 25 Nov 1996 15:35:18 -0800 Received: from noc.tc.umn.edu (0@noc.tc.umn.edu [128.101.131.1]) by net.bio.net (8.6.12/8.6.6) with SMTP id PAA19085; Mon, 25 Nov 1996 15:35:01 -0800 Received: from puccini.crl.umn.edu by noc.tc.umn.edu; Mon, 25 Nov 96 17:35:34 -0600 Received: from [205.212.60.161] by puccini.crl.umn.edu (SMI-8.6/SMI-SVR4) id RAA26477; Mon, 25 Nov 1996 17:39:26 -0600 Message-Id: <199611252339.RAA26477@puccini.crl.umn.edu> Mime-Version: 1.0 Content-Type: text/plain; charset="us-ascii" Date: Mon, 25 Nov 1996 17:34:28 -0600 To: rust-mil@net.bio.net, paulz@puccini.crl.umn.edu From: paulz@puccini.crl.umn.edu (Paul Zambino) Subject: Re: quantification-bean rust inoculum >dear rust-mil netters, >although i know that this newsgroup is devoted to rust and mildew of >cereals, i am posting this mail as i am working with rust (Uromyces >appendiculatus) of common bean. Rusts and mildews of other hosts are also discussed here, though not as often as I would like to see! > >Problem: >i would like to quantify my inoculum so that i know the amount of >urediniospores that go into each inoculation, and then standardize >the procedure. currently for bean rust, i have seen that the >urediniospores are weighed (mg/ml), suspended in distilled water >containing tween 20. but the problem is that the urediniospores are >black in color making it difficult to discern between the spores and dirt >(even after filtering through a sieve). Can we quantify spores by using >something like (a large (?) haemocytometer) as is used for tan spot or >white rust. >Is there a better way, that is used for cereals that i can adapt for bean >rust. The trouble with using a hemacytometer is that the spores often do not distribute themselves well when suspended in water, even with the Tween. You might be able to get away with using a hemacytometer if you use a different dispersant, but you will have to check for toxicity on the plant. Most cereal workers use a light mineral oil called Soltrol, but for my current work on white pine blister rust Soltrol is toxic to Ribes. From my experience, even dilute Tween causes germ tube distortion, and I don't know if it also affects infection. I suspend urediniospores in a dilute Tween solution, spin them down to get rid of "floaters", pour off the Tween, and resuspend by stirring (not vortexing) in 0.07 % agar. The agar keeps the spores from settling out and keeps the spores hydrated. Use VERY pure water for all steps! I would eliminate the hemacytometer altogether and try to get a more accurate measure of the spores that you are spraying or applying in other manner, to see what the potential load of viable spores is. I use a cheap perfume bottle aspirator from WalMart (available around the holidays!), take off the bulb, hook it up to the "supply side" of a vacuum pump, and spray away with my spore suspension. While you are inoculating, you can then spray part of the suspension on a 1 % agar plate, incubate at an appropriate temperature, and scan a number of microscope fields to count the number of spores per field. You will need to find the area of the microscope field, so first determine its diameter, using a stage micrometer or even the fine lines on a hemacytometer. Having an ocular grid also helps to keep track of spores when you are counting them. If you are interested, I can send you a paper copy of all of the protocols i am using for white pine blister rust, including individual inoculation/isolation chambers for single leaf cuttings or plants, and storage techniques adapted from techniques i learned at the USDA Cereal Rust Lab at St. Paul. > >also is there a way to collect spores instead of scraping them of the >leaves, which is very messy and leading to loss of the spores. >any pointers or references would be helpful You should contact some of the researchers on cereal rusts at NDSU, such as Brian Steffenson or Glen Stadler from Plant Pathology, or Dick Staples from USDA ARS. They have vacuum collection devices that work very well! Also Soltrol to see if it will work in your situation. And a lot of very useful knowledge! > >thanks, > >Kal > >kalavach@plains.nodak.edu >dept. of plant science >ndsu, fargo, nd The best of luck on your project! Paul Paul Zambino, Ph.D. USDA Forest Service Forestry Sciences Lab 5985 Highway K Rhinelander, WI 54501 PH:(715)362-1178 FAX: (715)362-1166 EMAIL: paulz@puccini.crl.umn.edu DG:p.zambino:R09F06A