From BIOSCI-REQUEST  Thu Feb  5 11:20:51 1998
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From: jtsr-stock@jtsr-stock.com
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Date: Thu, 05 Feb 98 10:11:00 EST
To: Friend@public.com
Subject: HOT STOCK-Double Your Money Before Easter!!
Message-ID: <>

You have been carefully selected to receive the following as a person obviously interested 
in this subject based upon your previous internet postings, or visits to one of our affiliate web
sites.  If you have received this message in error, please accept our apology as a 
responsible e-mailer, and reply with the word REMOVE in the subject line. You will be 
automatically excluded from future e-mailings. Thank you for your consideration and help
in making the Internet spam-free. *****

Visit http://www.jtsr-stock.com or call 888-295-6365 For Complete 
Information.

Who said opportunity only knocks once. JT's Restaurant is about to be one
of the fastest growing Restaurant chains. Under valued stock situations
presently $2.25 per share. Analysts predict stock to go as high as $5.00 
by Easter and could go up to $10.00 by end of 1998. Stock symbol JTSR on 
NASDAQ.  If you had invested just $100.00 in 1955 in Mcdonalds, it would 
be worth over $100 million today.  $1000 would be worth over $1 billion.  
Here is another opportunity for you.  Look for some big news soon to boost
the price of this stock.

Visit us at http://www.jtsr-stock.com or call 1-888-295-6365.




From BIOSCI-REQUEST  Thu Feb  5 14:25:19 1998
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From: "james.brown" <james.brown@bbsrc.ac.uk>
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Subject: COST 817 web site
To: rust-mil@net.bio.net
Message-Id: <980205161517.687@mserv.jic.bbsrc.ac.uk.0>
X-Dmw-Body-Names: COST 817 web site
Date: Thu, 5 Feb 98 16:15:17 +0000
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Two items have been added to the COST 817 web site today:

1. A 'Report of European Cereal Pathogen Virulence Surveys for 1995
and 1996': http://www.uea.ac.uk/nrp/jic/c817news.htm

2. An invitation to a discussion meeting on 'Future Perspectives of
Diversification Strategies', to be held in Edinburgh on 9th Aug 1998
(the day before the International Congress of Plant Pathology). Start
from: http://www.uea.ac.uk/nrp/jic/c817futr.htm

James Brown

From BIOSCI-REQUEST  Thu Feb  5 18:55:48 1998
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	Thu, 5 Feb 1998 20:54:47 -0600 (CST)
Message-ID: <34DA7AF5.FD5@tamu.edu>
Date: Thu, 05 Feb 1998 20:52:27 -0600
From: "Dr. Daniel Ebbole" <d-ebbole@tamu.edu>
Organization: Texas A&M University
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To: rust-mil@net.bio.net
Subject: Fungal Ecology Faculty Position
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Fungal Ecologist

	The Department of Plant Pathology and Microbiology of Texas A&M
University, College Station, invites applications for a 12-month tenure
track Assistant Professor position to study the ecology of fungal plant
pathogens.  The individual filling this position will join the strong
interdepartmental graduate Program for the Biology of Filamentous Fungi
that is supported by an NSF graduate training grant and a developing
area of faculty strength in the biological control of plant diseases. 
It is expected that a grant-supported research program will be
established based upon an important Texas disease problem.  Teaching
responsibilities can include graduate teaching and/or teaching in our
expanding undergraduate Bioenvironmental Sciences degree program.  Send
a letter of application, curriculum vitae, a statement of research and
teaching goals, and three letters of reference to:  Fungal Ecology
Search Committee, Department of Plant Pathology and Microbiology, Texas
A&M University, College Station, TX 77843-2132 (d-ebbole@tamu.edu).  For
information concerning our department see:
http://cygnus.tamu.edu/PLPA/dept_toc.html.  Screening of applications
will begin in March.  An equal employment opportunity employer.


-- 
Daniel Ebbole
Associate Professor
Program for the Biology of Filamentous Fungi
Dept of Plant Pathology and Microbiology
Texas A&M University
College Station, TX 77843-2132
USA
Ph.409-845-4831
Fax.409-845-6483

From BIOSCI-REQUEST  Tue Feb 10 02:00:05 1998
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Date: Tue, 10 Feb 1998 02:00:03 -0800
From: BIOSCI Administrator <biohelp>
Message-Id: <199802101000.CAA11963@net.bio.net>
To: rust-mil@net.bio.net
Subject: BIOSCI/bionet miniFAQ & Fundraiser

(LAST REVISION: 30-JUL-95)

This BIOSCI "miniFAQ" is designed to answer the questions that come up
the *most frequently*.  The main BIOSCI FAQ (Frequently Asked
Questions) is accessible on the World Wide Web at URL
http://www.bio.net/.

If you can not find an answer to your question in this or other
documentation, the BIOSCI technical support staff answers e-mail
queries sent to

		       biosci-help@net.bio.net

We can only answer questions about the use of the newsgroups and
mailing lists.  We unfortunately do not have the staff to do Internet
information searches or answer scientific questions.  Please post
those to the appropriate BIOSCI/bionet newsgroups.


	Contents:
	--------
	0) BIOSCI NEEDS YOUR SUPPORT!!

	1) Using the WWW to access the BIOSCI/bionet newsgroups.

	2) What to do about "spams," i.e., junk mail, ads, etc.

	3) Examples of subscribing and unsubscribing to the mailing lists.

	4) The BIOSCI user address and research interest directory.


0) BIOSCI NEEDS YOUR SUPPORT!!
------------------------------
BIOSCI's government funding has been expended, and we are now
operating solely from advertising revenue that we have raised from our
Web site at http://www.bio.net/.  We need just a few minutes of your
time to help us serve you.

You can do two important things which will take very little time for
you individually and will immensely help us continue to help you.

First, please use our WWW system at http://www.bio.net/ to access the
archives.  You can post or reply to messages via your Web browser as
described in item #1 below.  Your usage helps attract sponsors. If you
contact any of our sponsors, please be sure to thank them for
supporting BIOSCI. It is critical for them to get this feedback if
they are to continue their sponsorship for the long term.

Second, if you work for a company or organization that provides
products or services of interest to the biology community, please pass
this message on to your marketing or marketing communications
department or other appropriate group.  Please ask them to help
support BIOSCI by sponsoring our Web site and explain the uses and
benefits of the system to the biology community. If they are
interested, they can then contact us for further information at our
tech support address, biosci-help@net.bio.net.


1) Using the WWW to access the BIOSCI/bionet newsgroups.
--------------------------------------------------------
As of 10 December 1995, all BIOSCI/bionet full newsgroups are
accessible through the World Wide Web (WWW) at URL http://www.bio.net.
One can read and reply publicly or privately to both recent postings
and archived messages through one's Web browser if it is configured
properly to send e-mail.  Each newsgroup is equipped with its own WAIS
index.  The main BIOSCI home page also has access to the BIO-JOURNALS
Table of Contents database WAIS index and the BIOSCI user address
database described in another item further below.


2) What to do about "spams," i.e., junk mail, ads, etc.
-------------------------------------------------------
BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups),
mailing lists, and a hypermail archive at URL http://www.bio.net/.
The same postings are distributed on all media (except for a small
number of mailing-list-only groups at net.bio.net).  Unfortunately it
is becoming a despicable practice on the Internet (by a few people out
to make a fast buck) to do automated mass postings to thousands of
newsgroups and mailing lists.  These attempts to grab free advertising
are refered to as "spams" in the usual, somewhat boneheaded, net
terminology.  USENET is more susceptible to this practice, and many
spams originate on the USENET groups and then are passed on to the
mailing lists.  However, spammers also get lists of mailing addresses
and hit these too, so neither medium is immune.

What should you do personally if you get junk mail?
---------------------------------------------------
Just delete it and move on without reading it further.  Filing a
protest is becoming increasingly useless because spammers are often
disguising the addresses where the messages are sent from.  Unless you
really understand Internet mail systems, your attempt at protest by
sending replies to the message will often end up being sent to the
address of an innocent person that the spammer is victimizing.

What can BIOSCI/bionet do to protect its newsgroups?
----------------------------------------------------
The only solution currently available is to moderate the newsgroup.
If this newsgroup is already moderated, then you are in good shape.
Moderation protects the USENET distribution from about 95% of the
spams that are being sent to date and protects the mailing lists
completely.  Moderation means, however, that someone has to take the
time to review each message before it goes out.  We have set up
software here that simply allows the moderator to forward to an
address at net.bio.net messages that (s)he wishes to have distributed.
This takes no more time than that needed to read the message and pass
it on, say about 1 min. per message.

Most newsgroups currently have a discussion leader who is responsible
for their newsgroup.  The discussions leaders and their e-mail
addresses are listed in the BIOSCI Information Sheet which is
available on the Web at http://www.bio.net/.  If a newsgroup is being
hit with too many junk postings, please contact the discussion leader
for that group and see if there is interest in moderating the group.
Please do not assume that by simply posting a complaint to the
newsgroup itself, anyone on the BIOSCI staff will act on your
complaint.  With close to 100 newsgroups to run, the BIOSCI staff has
to rely on the discussion leaders of each newsgroup to report problems
directly to us at biosci-help@net.bio.net.

We will moderate any of our newsgroups if the discussion leader tells
us that the readership of the group wishes to do so and if a moderator
is willing to do the work.  For most BIOSCI/bionet groups, this
entails only a few minutes of work each day.

Moderating a newsgroup will resolve probably 95% of the junk postings
on the USENET distribution.  Unfortunately there are easy ways for
determined spammers to override the moderation mechanism on USENET,
but we can protect our e-mail subscribers from unwanted postings if
the newsgroup is moderated.  You can also access our newsgroups over
the WWW at URL http://www.bio.net.  While this Web interface will not
stop spammers from trying to post to the groups, this will give you
yet another way, besides using USENET news, to keep the junk out of
your personal mail files.  For those of you with local USENET news
systems, the Web interface will also give you faster access to new
newsgroups and recent postings.


3) Examples of subscribing and unsubscribing to the mailing lists.
------------------------------------------------------------------
PLEASE NOTE: The BIOSCI management does NOT act on
subscription/unsubscription requests that are posted improperly to the
newsgroups and mailing lists.  People who do this only bother everyone
on the lists to no avail.  Please be sure to follow the proper
procedures below.

Gory details are in the BIOSCI Information sheets on the Web at
http://www.bio.net.  Below we give an example utilizing the
METHODS-AND-REAGENTS list at both of our two BIOSCI sites:

Users in the Americas and Pacific Rim countries who use the BIOSCI
------------------------------------------------------------------
node at computer net.bio.net:
----------------------------

A) Determine the "listname" which is the <=8 character mail address
                                         ^^^^^^^^^^^^^
   for the group.  These can be found in the BIOSCI Info. Sheet.  For
   the METHODS-AND-REAGENTS group the mailing address is
   methods@net.bio.net.  The listname is the portion of the address to
   the left of the @ sign, i.e., "methods".  The listname is used with
   the "subscribe" and "unsubscribe" commands illustrated below.

B) Mail all commands in the body of a mail message addressed to
   biosci-server@net.bio.net.  Do NOT send commands to the newsgroup
   posting addresses!  Leave the Subject: line blank, any text on it
   will be ignored.

C) In the body of your message put one or more of the following
   commands with an "end" command on the last line, e.g.,

   subscribe methods
   unsubscribe methods
   end

   Do NOT put your e-mail address or other text on these lines.  The
   server only allows you to cancel your subscription if the address
   on your mail header matches the address on our mailing list.
   Please ask for help at biosci-help@net.bio.net if your address has
   changed, e.g., if you know you are on the list but the server tells
   you that you are not a member.


Users in Europe, Africa, and Central Asia who use the BIOSCI node at
--------------------------------------------------------------------
computer daresbury.ac.uk (also known as dl.ac.uk):
-------------------------------------------------

To subscribe and unsubscribe to/from the BIOSCI lists, you need to
specify the full USENET newsgroup name with "bionet-news." prepended.
The USENET newsgroup names are listed in the BIOSCI Information sheet
on the Web at http://www.bio.net/.  For the METHODS-AND-REAGENTS list
the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the
appropriate commands are

    sub bionet-news.bionet.molbio.methds-reagnts

    unsub bionet-news.bionet.molbio.methds-reagnts

These commands are included in a message addressed to mxt@dl.ac.uk,
NOT to the newsgroup mailing addresses.  As usual, include the text in
the body of the message as text on the Subject: line is ignored.

To unsubscribe from all the lists at the UK node, use

    unsub bionet-news

Please note that if the address in the list is different than the one
in your mail message header, you will not be able to unsubscribe by
this method. If you have problems, please mail biosci@daresbury.ac.uk.


4) The BIOSCI user address and research interest directory.
-----------------------------------------------------------
Please take this opportunity to add your name, address, and research
interest information to the BIOSCI User Address Database if you have
not already done so.

You can fill out the address form directly through our Web page at URL
http://www.bio.net/adrform.html.

The address database is reindexed nightly for WWW access (the URL is
http://www.bio.net/).  If you are not directly on the Internet but can
reach it by e-mail, please use our waismail server to access the user
directory.  waismail use is described above.  You can also request a
user address form by e-mail from biosci-help@net.bio.net.

Please check your database entry from time-to-time to see if your
address information is still up-to-date.  Because of our limited
personnel resources, we ask that you resubmit a *complete* form to
revise your entry; we only replace complete entries and do not have
resources to edit old forms.


From BIOSCI-REQUEST  Thu Feb 12 21:19:16 1998
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Date: Fri, 13 Feb 1998 15:08:20 +1000
To: R.SINGH@CGNET.COM, rust-mil@net.bio.net
From: BOB McINTOSH <bobm@camden.usyd.edu.au>
Subject: Yr Genes

Reaction to P. striiformis

The following genes for resistance to stripe/yellow rust are being designated:

Yr27 (9889).  [YrSk (9649)].  2BS (9889).	v: Ciano 79 (9889); Selkirk
(9889). Yr21 is
	present in many CIMMYT wheat lines (9889) and possibly Webster.
				Yr27 is closely linked with Lr13 (repulsion).

Yr28 (9890).   4DS (9890)	v: Synthetic = Altar 84/ T.tauschii W-219.
Synthetic/ Opata 85 SSD
	lines 25,47,57,67,84,87,89 (9890).
			dv: T. tauschii W-219 (9890).

Gene Yr22 was also reported for chromosome 4D but in the absence of an
appropriate single gene stock and the unavailability of avirulent cultivars
in most laboratories, linkage test data are unlikely to be available in the
foreseeable future.

Reaction to Puccinia recondita

Lr17a (9891).	[Lr17].

Lr17b (9891).	[LrH(  ), WBR2 (9892)].	v: Harrier (9891); Norin 10 -
Brevor, 14 (9891);
	Maris Fundin (9891). Hobbit Sib = Dwarf A Lr13 (9891).

Please lodge objections or comments by 1 March 1998 to R.A. McIntosh.

9889.  McIntosh RA et al. 1998. Personal communication

9890.  Singh RP et al. 1998. Personal communication

9891.  Singh D, Park RF & McIntosh RA. 1998. Personal communication

9892.  Jones ERL & Clifford BC. 1996. Annual Report - U.K. Cereal Pathogen
Virulence survey.

Prof. R.A. McIntosh
University of Sydney
Plant Breeding Institute - Cobbitty
PMB 11
Camden NSW 2570
Australia

P:  +61 02 9351 8802 (direct line)
P:  +61 02 9351 8800 (switch/answering machine)
F:  +61 02 9351 8875
E:  bobm@camden.usyd.edu.au



From BIOSCI-REQUEST  Fri Feb 13 13:03:33 1998
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From: etmp@etmp.com
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	  id QAA12760; Fri, 13 Feb 1998 16:00:19 -0500
Message-Id: <199802132100.QAA12760@apollo.redcomet.net>
To: Friend@public.com
Date: Fri, 13 Feb 98 13:47:23 EST
Subject: Eventemp Stock(ETMP) Heating Up - Huge Orders Coming In!!

 You have been carefully selected to receive the following as a
 person obviously interested in this subject based upon your
 previous internet postings, or visits to one of our affiliate web
 sites.  If you have received this message in error, please accept
 our apology as a responsible e-mailer, and reply with the word
 REMOVE in the subject line. You will be automatically excluded
 from future e-mailings. Thank you for your consideration and help
 in making the Internet spam-free. *****

*** News Flash *** 01/30/98 - Eventemp receives 27 million dollar contract - Company 
expects net profit of 5 to 6 million dollars.  Other large contracts pending.  Huge profit
potential.

Analyst say look for Eventemp (Symbol OTC BB: ETMP) to go as high as $10.00
by March 1.  Analysts predict that stock could shoot to $6.00 in the next few days.
Stock rose over 41% on 01/30/98 - Get in while there is still time.

For complete info, visit http://www.etmp.com

Now you can call your car from any available telephone and start 
the air conditioner or heater and have it pre-cooled or pre-heated for
your comfort when you arrive....WITH THE VEHICLE'S ENGINE STILL 
OFF! 

THE TECHNOLOGY EXISTS!
THE SYSTEM WORKS!
THE COMPANY IS EVENTEMP!
THE STOCK (OTC BB: ETMP) currently trades at about $4.25 and could
possibly reach $10.00 by March 1, 1998!

EVENTEMP owns the exclusive patent rights to this revolutionary system
which will be available as an option on luxury cars this year. Deals are 
in the works to make this available on all new cars by the year 2000!
Look for some exciting news to be released soon!

Visit EVENTEMP'S web site at http://www.etmp.com for more
information!

The temperature in your car may be cool, but this stock is going to be one
of the HOTTEST of 1998!


From BIOSCI-REQUEST  Mon Feb 16 01:20:15 1998
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Date: Mon, 16 Feb 1998 10:20:32
To: rust-mil@net.bio.net
From: Helge Skinnes <helge.skinnes@nlh10.nlh.no>
Subject: Virus
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"
Content-Length: 730

Dear everybody.

I just got a warning letter about chain letter, junk mail etc that MAY
CONTAIN VIRUS:

Good Times, Good Times Spoof, Ghost, PENPAL
GREETINGS!, Make Money Fast, NaughtyRobot, AOL4FREE, Join the Crew, Death
Ray, A.I.D.S. Hoax, Internet Cleanup Day,.........

Due to the information I got, the E- mail does not contain any virus that
start by itself whithout your particiation, but the attachment file(s) may
contain virus. 
If you want to know more about these chain letters look at: 

http://ciac.llnl.gov/ciac/CIACHoaxes.html
http://www.kumite.com/myths/home.htm

Someone have, however, warned us, NOT TO OPEN E- mail wich is suspicicious,
but to clear(erase) it without opening it.

Best wishes,

Helge Skinnes


From BIOSCI-REQUEST  Mon Feb 16 19:16:14 1998
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Message-Id: <199802170319.MAA09433@sansui.bio.mie-u.ac.jp>
Date: Tue, 17 Feb 1998 12:12:09 +0900
To: rust-mil@net.bio.net
From: takamatu@bio.mie-u.ac.jp (TAKAMATSU Susumu)
X-Sender: takamatu@sansui.bio.mie-u.ac.jp
Subject: List of the host plants of powdery mildew fungi
Cc: takamatu@bio.mie-u.ac.jp
MIME-Version: 1.0
Content-Type: text/plain; charset=iso-2022-jp
X-Mailer: Eudora-J(1.3.8.5-J13)

Dear Colleague,

A data base of the list 'Host Plants of Powdery Mildew Fungi and Their
Distribution by Country' content in the book 'Host Range and Geographical
Distribution of the Powdery Mildew Fungi' (Japan Scientific Society Press,
Tokyo, Japan) by Koji Amano (formerly Hirata) is now available for computer
processing and is being distributed without charge under kind permission of
the author and the publisher. The list covers 9838 host plant species of
the powdery mildew fungi and their distribution in the world. The files are
available at the WWW site:
http://sansui.bio.mie-u.ac.jp/seisan/byori/download.html
If you need floppy disk version, please contact me (Susumu Takamatsu) by
e-mail (takamatu@bio.mie-u.ac.jp) or letter to the address described below.


--------------------------------------------------------------
HOST PLANTS OF THE POWDERY MILDEW FUNGI DATA BASE  Ver. 1.0

------------------------------
INTRODUCTION
------------------------------
        Since the book of "Host Range and Geographical Distribution of the
Powdery Mildew Fungi" (Japan Scientific Society Press, Tokyo, Japan) was
published by Dr. Koji Amano (formaerly Hirata) in 1986, the book has
received great reputation due to the scientific and practical contribution
in this field all over the world. The list content of "Host Plants of
Powdery Mildew Fungi and Their Distribution by Country", a part of this
book, is particularly the useful and valuable guidline for the research and
researchers, because of long-year products of accurate and detailed survey
of the literatures on host plants and powdery mildew fungi. The data base
of the list content in the book is now available for computer processing,
which can lead to simpler and more time-saving survey. Anyone can access to
this data base covering 9838 host plant species of the powdery mildew fungi
(9176 dicots and 662 monocots) listed in the book of Dr. K. Amano.

        As the data source has been extensively added after the publication
of this book, we would like to be responded with the supplementation of
data published in the last decade. It is greatly appreciated if you kindly
inform them to us by sending reprint or copy of the concerned reports. We
also would like to thank you for telling us any mistake and inconvenience
in the present version of the data base.

        We are grateful to Dr. Koji Amano and Japan Scientific Society
Press for kind permission of constructing and offering to public use of
this data base. Some students of Lab. of Plant Pathology, Mie University,
assisted us to construct the data base. These include Masao Arakawa,
Tetsuya Hirata, Yukiko Komiya, Tomohiro Mitsui and Hanako Ito.

---------------------------------------
CONTENTS (6 FILES)
---------------------------------------
        This data base contains six files, in which three kinds of file
format are available as data base.

1. Readme (Japanese)
2. Readme (English) --- this file
3. PMhost.excel.sylk
        Microsoft Excel 5.0 file.
        The list content "Host Plants of Powdery Mildew Fungi and Their
Distribution by Country", a part of the book "Host Range and Geographical
Distribution of the Powdery Mildew Fungi" (by Dr. K. Amano, Japan
Scientific Society Press, Tokyo, Japan,1986) was converted into Excel file.
You are noted that more than one cell were allotted to the name of counries
as needed. The country names were described according to the Amano's book
in 1986.
4. PMhost.filemaker
        Filemaker Pro 3.0 file.
        Several modification of the data description was made on the Excel
file and converted to Filemaker described as follows; 1) It has been
processed to deleate synomyns of plant listed as supplemented data in the
original description, since the procedure of surveying the list by computer
has been simplified by the deleating. 2) Abbreviations of the generic name
of plants and fungi were converted into full spell. 3) Card layout and
table layout are available in the data base.
5. PMhost.text
        text file
        The data content in the PMhost.filemaler was conveted into text
file. you can convert the file into another file format that is available
for your computer.
6. reference.word
        Microsoft Word 5.1 file
        Reference list which are included in the Amano's book. These
references are available at The National Science Museum of Japan, because
most of them were donated there by Dr. K. Amano. 

----------------------------------------------------
HOW TO OBTAIN THE BOOK
----------------------------------------------------
        The book "Host Range and Geographical Distribution of the Powdery
Mildew Fungi" (K. Amano, 1986, Japan Scientific Society Press, Tokyo,
Japan) is available now. Please contact the following publisher. The price
is 10,000 Yen, not including postage cost.
        JAPAN SCIENTIFIC SOCIETIES PRESS
        2-10 Hongo, 6-chome, Bunkyo-ku, Tokyo 113-0033, Japan
        TEL +81-33814-2001
        FAX +81-33814-2002

---------------------------------------------------
COPYRIGHT AND AUTHORS
---------------------------------------------------
        Copyright of contents of the data base belongs to Dr. Koji Amano
and Japan Scientific Societies Press, and the data base to Susumu Takamatsu
and Yukio Sato.
        Feel free to distribute this data base to anyone who needs it.
Modification and/or addition of data is prohibited by the authors to
prevent confusion of the data base. When you want to supplement or modify
the data, please contact S. Takamatsu or Y. Sato with providing reprint or
copy of the reference.
        It is greatly appreciated if you send any impression, error
informations, supplement data, questions or ideas to S. Takamatsu or Y.
Sato by e-mail or any convenient way.

Susumu Takamatsu
Laboratory of Plant Pathology, Faculty of Bioresources, Mie University,
1515 kamihama, Tsu 514, Japan
TEL: +81-59-2319497
FAX: +81-59-2319637
e-mail: takamatu@bio.mie-u.ac.jp

Yukio Sato
Toyama Prefectural University
5180 Kurokawa, Kosugi-machi, Toyama 939-03, Japan
TEL: +81-766-567500
FAX: +81-766-560396
e-mail: ysato@pu-toyama.ac.jp



*********************
Susumu Takamatsu

Laboratory of Plant Pathology,              TEL +81-59231-9497
Faculty of Bioresources,                        FAX +81-59231-9637
Mie University, Tsu 514-8507,               e-mail takamatu@bio.mie-u.ac.jp
Japan


From BIOSCI-REQUEST  Sun Feb 22 14:03:06 1998
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From BIOSCI-REQUEST  Wed Feb 25 23:33:39 1998
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Date: Thu, 26 Feb 1998 08:32:26 +0100
From: "David B. Collinge" <David.B.Collinge@plbio.kvl.dk>
To: rust-mil@net.bio.net
Subject: Rust-mildew net: biotrophy versus obligate
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We had a rather heated discussion yesterday in the department about the
term biotrophy as part of our Ph.D. course in plant pathology.

One (the conventional/conservative) view is that a pathogen has to be
obligate to be a biotroph.

The other view is that the two terms actually describe different properties.
Being obligate is actually an attribute independent of biotrophy since
biotrophy concerns the way in which the pathogen extracts nutrients from
the host (i.e by not killing it first) whereas being obligate merely describes
our inability to culture the organism in the absence of the host. 

It does not help the progresive cause that Agrios favours the conservative
view.

Cladosporium fulvum was the issue of contention. Of course, none of us
actually work with this organism but stick to non-controversial beasties like
Blumeria graminis, but that does not seem to prevent people from having
entrenched views.

I would be interested in hearing views on this matter.

david

David B. Collinge
Associate Professor
Dept. of Plant Biology,
Royal Veterinary and Agricultural University,
Thorvaldsensvej 40,
1871 Frederiksberg C,
Denmark.

email: 	dbc@kvl.dk 
http://www.plbio.kvl.dk/~dacoj3/index.html

fax 	+45 35283310
tel  	+45 35283356


From BIOSCI-REQUEST  Thu Feb 26 06:05:57 1998
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Date: Thu, 26 Feb 1998 15:19:11 +0100
From: Richard Oliver <rpo@crc.dk>
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David B. Collinge wrote:

> We had a rather heated discussion yesterday in the department about
> the
> term biotrophy as part of our Ph.D. course in plant pathology.
>
> One (the conventional/conservative) view is that a pathogen has to be
> obligate to be a biotroph.
>
> The other view is that the two terms actually describe different
> properties.
> Being obligate is actually an attribute independent of biotrophy since
>
> biotrophy concerns the way in which the pathogen extracts nutrients
> from
> the host (i.e by not killing it first) whereas being obligate merely
> describes
> our inability to culture the organism in the absence of the host.
>
> It does not help the progresive cause that Agrios favours the
> conservative
> view.
>
> Cladosporium fulvum was the issue of contention. Of course, none of us
>
> actually work with this organism but stick to non-controversial
> beasties like
> Blumeria graminis, but that does not seem to prevent people from
> having
> entrenched views.
>
> I would be interested in hearing views on this matter.
>
> david
>
> Here we go again.

In Hollidays Dictionary he quotes Lewis 1973 "an organism entirely
dependant upon another living organism as a source of nutrients". I
would put Cladosporium in this because it grows on healthy living tomato
leaf tissue. Clad can also grow in culture of course and that's why we
work on it. Erysiphe (to reopen another wound) is obligate and
biotrophic. We all agree on that. But what if someone found out how to
make it grow in culture. Would that make it a necrotroph. I think not.
The underlying assumption of biotrophy is unfettered growth by the
pathogen in a unresponding and unaware plant - the cat burglar analogy.
It has been clear for many years now that plants in compatible
biotrophic reactions do display resistance but that it is too slow or
too weak to affect the outcome. This is presumably why we see degrees of
resistance. There is a fight going on and the fight can be hotter
-necrotrophic - or colder - biotrophic

--
Professor Richard Oliver
Carlsberg Laboratory, Dept. of Physiology
Gamle Carlsberg Vej 10, DK-2500 Valby, Denmark
Phone: +45 33 27 52 25, Fax: +45 33 27 47 66,
PLEASE NOTE NEW Email: rpo@crc.dk



From BIOSCI-REQUEST  Thu Feb 26 06:42:10 1998
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From: "Michele C. Heath" <HEATH@botany.utoronto.ca>
Organization:  University of Toronto Botany
To: rust-mil@net.bio.net
Date:          Thu, 26 Feb 1998 09:36:13 EST
Subject:       Re: Rust-mildew net: biotrophy versus obligate
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Dear David,

Re: biotrophy.

>From its derivation, the term biotrophy relates purely to the mode of 
nutrition of the organism - i.e. it gets its food from living host 
cells (see papers in the 1970's by David Lewis). Therefore, it is 
possible to have "hemibiotrophic" parasites, as described 
by Luttrell, that switch from a biotrophic mode of parasitism to a 
necrotrophic mode during their life cycle (e.g. Colletotrichum 
lindemuthianum). These hemibiotrophs grow easily in culture. 

To define as biotrophic only organisms that are obligate symbionts is 
to take away the usefulness of the term. For example, I have found 
that even some so-called necrotrophs can grow for short periods of 
time in living host cells; if the parasite is obtaining food from the 
living cell, then as far as I am concerned, it is growing 
biotrophically.  From a cell biology point of 
view, it is obvious that organisms that grow in living tissue, and 
particularly inside living cells, have some special features as 
a group. These include preventing the hypersensitive response 
as well as other defenses. As well, biotrophs tend to 
induce significant cellular changes in susceptible host cells that 
presumably facilitate nutrient acquisition. 

Whether we can grow these organisms in culture is irrelevant to the 
definition of biotrophy regardless of what Agrios states.  The fact 
that some biotrophs ARE difficult to culture is an indication that 
their interaction with their hosts has evolved to such a degree that 
their continued development is linked to signals from the plant. 

I am not aware of anyone who works on the cell biology of biotrophs 
who considers that a biotroph has to be an obligate parasite. Thus, 
the colleagues in my department who work with Cladosporium fulvum 
consider the fungus to be a biotroph.


Michele Heath
****************

Michele C. Heath, Ph.D. F.R.S.C.
Professor, Botany Department,
University of Toronto,
25 Willcocks St., Toronto,
ON M5S 3B2, Canada.
tel: 416 978 6304
Fax: 416 978 5878
email:heath@botany.utoronto.ca

From BIOSCI-REQUEST  Thu Feb 26 06:44:59 1998
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Date: Thu, 26 Feb 1998 15:43:21 +0100
From: "Bob O'Hara" <bob.ohara@risoe.dk>
Subject: Re: Rust-mildew net: biotrophy versus obligate
In-reply-to: <34F579DF.E9F9E774@crc.dk>
To: Richard Oliver <rpo@crc.dk>, rpo@crc.dk, rust-mil@net.bio.net
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In the bits of Richard's meassage I have chopped out, I agree with 
Richard.  Then...

> The underlying assumption of biotrophy is unfettered growth by the
> pathogen in a unresponding and unaware plant - the cat burglar analogy.
> It has been clear for many years now that plants in compatible
> biotrophic reactions do display resistance but that it is too slow or
> too weak to affect the outcome. This is presumably why we see degrees of
> resistance. There is a fight going on and the fight can be hotter
> -necrotrophic - or colder - biotrophic
> 
I always got the impression that necrotrophs meant to kill their host 
before feeding on it.   In which case, an unresponding and unaware 
plant would still have bits of it going brown and dropping off.  My 
impression is that the resistant plants don't show necrosis, whereas 
the susceptible ones do, and ironic inversion of what Richard wrote.

Before anyone complains, I am simplifying the argument.  But I get 
the impression that Richard thinks that the HR is the main response 
to any pathogen.  If this were true, then the plant would only be 
helping the necrotrophs by expressing resistance!


Bob 

Bob O'Hara

Department of Plant Biology and Biogeochemistry
Ris=A2 National Laboratory                  bob.ohara@risoe.dk
DK-4000 Roskilde                          Tel: +45 46 77 41 12
Denmark                                   Fax: +45 46 77 41 22

First get your facts; and then you can distort them at your leisure

- Mark Twain

