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From: "james.brown" <james.brown@bbsrc.ac.uk>
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Subject: Fungicides to kill rust
To: rust-mil@net.bio.net
Message-Id: <980601102206.683@mserv.jic.bbsrc.ac.uk.0>
X-Dmw-Body-Names: Fungicides to kill rust
Date: Mon, 1 Jun 98 10:22:07 +0100
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Does anyone know of fungicides that will control basidiomycetes but
not ascomycetes? Specifically, I want to control yellow rust in a
trial of Septoria tritici on wheat. There used to be one called
benodanil (sold in the U.K. as Calirus) but it went out of production
some years ago. Are there any more recent products that I could use?
(This is an experimental trial, not for commercial production.)

James Brown
_______________________________________________________

Dr J. K. M. Brown,
Cereals Research Department, John Innes Centre,
Colney Lane, Norwich, NR4 7UH, England
Phone: (+44)(0) 1603 452571. Fax: (+44)(0) 1603 502241.
E-mail: james.brown@bbsrc.ac.uk

From BIOSCI-REQUEST  Mon Jun  1 07:42:20 1998
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 1 Jun 1998 09:39:25 CDT
Date: Mon, 01 Jun 1998 09:42:46 -0500
From: Brian Wickes <wickes@uthscsa.edu>
Subject: Post-Doc
To: rust-mil@net.bio.net
Reply-to: wickes@uthscsa.edu
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POST-DOCTORAL POSITION IN MEDICAL MYCOLOGY

A number of Post-Doctoral positions are available for studying the role
of mating-type associated genes in virulence of Cryptococcus neoformans,
an important human fungal pathogen. Projects include the isolation and
characterization of genes involved in mating, hyphal development, and/or
virulence. Molecular experience is required and a strong background in
yeast or fungal genetics/molecular biology is preferred. The positions
are located in the Dept. of Microbiology at the University of Texas
Health Science Center at San Antonio (UTHSCSA) and are funded for up to
three years.

The Health Science Center has one of the largest groups of
internationally recognized mycologists working on human fungal pathogens
and offers an excellent opportunity to enter the field of medical
mycology. The city of San Antonio is one of the ten largest in the
country yet is one of the most affordable to live in. Salary is highly
competitive. Applications may be sent by mail, fax, or e-mail and must
include the following: a detailed C.V., selected reprint(s), and contact
information for three references. Send to:

Brian Wickes, Ph.D.
Dept. of Microbiology, Rm 5.023V
7703 Floyd Curl Dr.
University of Texas Health Science Center at San Antonio
San Antonio, TX 78284-7758
Fax (210) 567-6612
e-mail wickes@uthscsa.edu

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From: "james.brown" <james.brown@bbsrc.ac.uk>
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Subject: Fungicides to kill rust
To: rust-mil@net.bio.net
Message-Id: <980603112820.681@mserv.jic.bbsrc.ac.uk.0>
X-Dmw-Body-Names: Fungicides to kill rust
Date: Wed, 3 Jun 98 11:28:20 +0100
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In case others are interested in the problem of controlling yellow
rusts but Septoria tritici in wheat:

Two people recommended oxycarboxin, a basidio-specific fungicide (the
UK Pesticide Guide for 1997 only lists activities against fairy rings
in turfgrass and against rusts in ornamentals). Oxycarboxin is made by
Uniroyal and sold in the UK as an emulsifiable concentrate,
Ringmaster, by RP Amenity (= subsidiary of Rhone-Poulenc) and as a
wettable powder, Plantvax 75, by Fargro.

Several people recommended Calirus, if one can get hold of the stuff
(it went out of production in the late '70s - someone kindly sent me a
sample, and the packet gives an application rate in pounds per acre!)

Either Calirus or an oxycarboxin fungicide, plus Calixin to control
mildew, would seem to be a good combination.

An agronomist recommended fenpropimorph (e.g. Corbel) + tridemorph
(e.g. Calixin) if one is prepared to tolerate limited damage to
Septoria.

James Brown
_______________________________________________________

Dr J. K. M. Brown,
Cereals Research Department, John Innes Centre,
Colney Lane, Norwich, NR4 7UH, England
Phone: (+44)(0) 1603 452571. Fax: (+44)(0) 1603 502241.
E-mail: james.brown@bbsrc.ac.uk

From BIOSCI-REQUEST  Sun Jun  7 09:42:18 1998
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Date: Sun, 7 Jun 1998 09:40:24 -0700
From: <mike2287@yahoo.com>
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Subject: Submit Your Site-400 Search Engines 7.50
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From BIOSCI-REQUEST  Wed Jun 10 02:00:08 1998
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Date: Wed, 10 Jun 1998 02:00:06 -0700
From: BIOSCI Administrator <biohelp>
Message-Id: <199806100900.CAA11127@net.bio.net>
To: rust-mil@net.bio.net
Subject: BIOSCI/bionet miniFAQ & Fundraiser

(LAST REVISION: 30-JUL-95)

This BIOSCI "miniFAQ" is designed to answer the questions that come up
the *most frequently*.  The main BIOSCI FAQ (Frequently Asked
Questions) is accessible on the World Wide Web at URL
http://www.bio.net/.

If you can not find an answer to your question in this or other
documentation, the BIOSCI technical support staff answers e-mail
queries sent to

		       biosci-help@net.bio.net

We can only answer questions about the use of the newsgroups and
mailing lists.  We unfortunately do not have the staff to do Internet
information searches or answer scientific questions.  Please post
those to the appropriate BIOSCI/bionet newsgroups.


	Contents:
	--------
	0) BIOSCI NEEDS YOUR SUPPORT!!

	1) Using the WWW to access the BIOSCI/bionet newsgroups.

	2) What to do about "spams," i.e., junk mail, ads, etc.

	3) Examples of subscribing and unsubscribing to the mailing lists.

	4) The BIOSCI user address and research interest directory.


0) BIOSCI NEEDS YOUR SUPPORT!!
------------------------------
BIOSCI's government funding has been expended, and we are now
operating solely from advertising revenue that we have raised from our
Web site at http://www.bio.net/.  We need just a few minutes of your
time to help us serve you.

You can do two important things which will take very little time for
you individually and will immensely help us continue to help you.

First, please use our WWW system at http://www.bio.net/ to access the
archives.  You can post or reply to messages via your Web browser as
described in item #1 below.  Your usage helps attract sponsors. If you
contact any of our sponsors, please be sure to thank them for
supporting BIOSCI. It is critical for them to get this feedback if
they are to continue their sponsorship for the long term.

Second, if you work for a company or organization that provides
products or services of interest to the biology community, please pass
this message on to your marketing or marketing communications
department or other appropriate group.  Please ask them to help
support BIOSCI by sponsoring our Web site and explain the uses and
benefits of the system to the biology community. If they are
interested, they can then contact us for further information at our
tech support address, biosci-help@net.bio.net.


1) Using the WWW to access the BIOSCI/bionet newsgroups.
--------------------------------------------------------
As of 10 December 1995, all BIOSCI/bionet full newsgroups are
accessible through the World Wide Web (WWW) at URL http://www.bio.net.
One can read and reply publicly or privately to both recent postings
and archived messages through one's Web browser if it is configured
properly to send e-mail.  Each newsgroup is equipped with its own WAIS
index.  The main BIOSCI home page also has access to the BIO-JOURNALS
Table of Contents database WAIS index and the BIOSCI user address
database described in another item further below.


2) What to do about "spams," i.e., junk mail, ads, etc.
-------------------------------------------------------
BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups),
mailing lists, and a hypermail archive at URL http://www.bio.net/.
The same postings are distributed on all media (except for a small
number of mailing-list-only groups at net.bio.net).  Unfortunately it
is becoming a despicable practice on the Internet (by a few people out
to make a fast buck) to do automated mass postings to thousands of
newsgroups and mailing lists.  These attempts to grab free advertising
are refered to as "spams" in the usual, somewhat boneheaded, net
terminology.  USENET is more susceptible to this practice, and many
spams originate on the USENET groups and then are passed on to the
mailing lists.  However, spammers also get lists of mailing addresses
and hit these too, so neither medium is immune.

What should you do personally if you get junk mail?
---------------------------------------------------
Just delete it and move on without reading it further.  Filing a
protest is becoming increasingly useless because spammers are often
disguising the addresses where the messages are sent from.  Unless you
really understand Internet mail systems, your attempt at protest by
sending replies to the message will often end up being sent to the
address of an innocent person that the spammer is victimizing.

What can BIOSCI/bionet do to protect its newsgroups?
----------------------------------------------------
The only solution currently available is to moderate the newsgroup.
If this newsgroup is already moderated, then you are in good shape.
Moderation protects the USENET distribution from about 95% of the
spams that are being sent to date and protects the mailing lists
completely.  Moderation means, however, that someone has to take the
time to review each message before it goes out.  We have set up
software here that simply allows the moderator to forward to an
address at net.bio.net messages that (s)he wishes to have distributed.
This takes no more time than that needed to read the message and pass
it on, say about 1 min. per message.

Most newsgroups currently have a discussion leader who is responsible
for their newsgroup.  The discussions leaders and their e-mail
addresses are listed in the BIOSCI Information Sheet which is
available on the Web at http://www.bio.net/.  If a newsgroup is being
hit with too many junk postings, please contact the discussion leader
for that group and see if there is interest in moderating the group.
Please do not assume that by simply posting a complaint to the
newsgroup itself, anyone on the BIOSCI staff will act on your
complaint.  With close to 100 newsgroups to run, the BIOSCI staff has
to rely on the discussion leaders of each newsgroup to report problems
directly to us at biosci-help@net.bio.net.

We will moderate any of our newsgroups if the discussion leader tells
us that the readership of the group wishes to do so and if a moderator
is willing to do the work.  For most BIOSCI/bionet groups, this
entails only a few minutes of work each day.

Moderating a newsgroup will resolve probably 95% of the junk postings
on the USENET distribution.  Unfortunately there are easy ways for
determined spammers to override the moderation mechanism on USENET,
but we can protect our e-mail subscribers from unwanted postings if
the newsgroup is moderated.  You can also access our newsgroups over
the WWW at URL http://www.bio.net.  While this Web interface will not
stop spammers from trying to post to the groups, this will give you
yet another way, besides using USENET news, to keep the junk out of
your personal mail files.  For those of you with local USENET news
systems, the Web interface will also give you faster access to new
newsgroups and recent postings.


3) Examples of subscribing and unsubscribing to the mailing lists.
------------------------------------------------------------------
PLEASE NOTE: The BIOSCI management does NOT act on
subscription/unsubscription requests that are posted improperly to the
newsgroups and mailing lists.  People who do this only bother everyone
on the lists to no avail.  Please be sure to follow the proper
procedures below.

Gory details are in the BIOSCI Information sheets on the Web at
http://www.bio.net.  Below we give an example utilizing the
METHODS-AND-REAGENTS list at both of our two BIOSCI sites:

Users in the Americas and Pacific Rim countries who use the BIOSCI
------------------------------------------------------------------
node at computer net.bio.net:
----------------------------

A) Determine the "listname" which is the <=8 character mail address
                                         ^^^^^^^^^^^^^
   for the group.  These can be found in the BIOSCI Info. Sheet.  For
   the METHODS-AND-REAGENTS group the mailing address is
   methods@net.bio.net.  The listname is the portion of the address to
   the left of the @ sign, i.e., "methods".  The listname is used with
   the "subscribe" and "unsubscribe" commands illustrated below.

B) Mail all commands in the body of a mail message addressed to
   biosci-server@net.bio.net.  Do NOT send commands to the newsgroup
   posting addresses!  Leave the Subject: line blank, any text on it
   will be ignored.

C) In the body of your message put one or more of the following
   commands with an "end" command on the last line, e.g.,

   subscribe methods
   unsubscribe methods
   end

   Do NOT put your e-mail address or other text on these lines.  The
   server only allows you to cancel your subscription if the address
   on your mail header matches the address on our mailing list.
   Please ask for help at biosci-help@net.bio.net if your address has
   changed, e.g., if you know you are on the list but the server tells
   you that you are not a member.


Users in Europe, Africa, and Central Asia who use the BIOSCI node at
--------------------------------------------------------------------
computer daresbury.ac.uk (also known as dl.ac.uk):
-------------------------------------------------

To subscribe and unsubscribe to/from the BIOSCI lists, you need to
specify the full USENET newsgroup name with "bionet-news." prepended.
The USENET newsgroup names are listed in the BIOSCI Information sheet
on the Web at http://www.bio.net/.  For the METHODS-AND-REAGENTS list
the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the
appropriate commands are

    sub bionet-news.bionet.molbio.methds-reagnts

    unsub bionet-news.bionet.molbio.methds-reagnts

These commands are included in a message addressed to mxt@dl.ac.uk,
NOT to the newsgroup mailing addresses.  As usual, include the text in
the body of the message as text on the Subject: line is ignored.

To unsubscribe from all the lists at the UK node, use

    unsub bionet-news

Please note that if the address in the list is different than the one
in your mail message header, you will not be able to unsubscribe by
this method. If you have problems, please mail biosci@daresbury.ac.uk.


4) The BIOSCI user address and research interest directory.
-----------------------------------------------------------
Please take this opportunity to add your name, address, and research
interest information to the BIOSCI User Address Database if you have
not already done so.

You can fill out the address form directly through our Web page at URL
http://www.bio.net/adrform.html.

The address database is reindexed nightly for WWW access (the URL is
http://www.bio.net/).  If you are not directly on the Internet but can
reach it by e-mail, please use our waismail server to access the user
directory.  waismail use is described above.  You can also request a
user address form by e-mail from biosci-help@net.bio.net.

Please check your database entry from time-to-time to see if your
address information is still up-to-date.  Because of our limited
personnel resources, we ask that you resubmit a *complete* form to
revise your entry; we only replace complete entries and do not have
resources to edit old forms.


From BIOSCI-REQUEST  Thu Jun 11 23:46:18 1998
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Message-ID: <71380DBD6E87D1119DF100805F8BF7220809FB@pc512.sp.dk>
From: =?iso-8859-1?Q?Mogens_St=F8vring_Hovm=F8ller?=
	 <Mogens.Hovmoller@agrsci.dk>
To: "'rust-mil'" <rust-mil@net.bio.net>
Subject: Joint COST817 WG1/WG4 and NJF (Nordic) meeting in Denmark, Novemb
	er 1998
Date: Fri, 12 Jun 1998 08:43:25 +0200
MIME-Version: 1.0
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At a COST817 MC-meeting in Praha, November 1997, it was decided to=20
arrange a joint WG1 and WG4 meeting in Denmark in November 1998, in=20
connection with a Nordic meeting on resistance biology of agricultural
crops.

The meeting will take place on Tune Landbrugsskole, Sealand (near=20
Roskilde/Copenhagen),  from November 25 (Wednesday) to November 27=20
(Friday) 1998. The costs are approximately DKK 795/day including all=20
ordinary meals and accommodation.=20

Registration fee for NJF-meeting (non-members):    DKK 1800

Registration fee for COST817-meeting: DKK 500

At day two, participants at the NJF-meeting are invited to join the
afternoon=20
COST-session without further registration fee, and likewise COST817=20
participants can join the NJF-morning session without fee.


Preliminary programme

	Wednesday 25. November
=09
	10:00-12:00  Arrival/registration=20

	12:00-13:00  Lunch

	Parallel sessions:
	13:00-17:00  Cost817 MC-meeting

	13:00-17:30  NJF: Diseases other than cereal mildews, rusts and=20
	Septoria. Short presentation 10-20 min, including discussion.
Call=20
	for contributions made June-July, finally programme in August.

	19:00	Dinner for all participants


	Thursday 26. November

	Arrival/ registration=09

	Cereal mildews, rusts and Septoria in Europe 1993-98; population
	dynamics and host resistance

	8:30-12:00	NJF working group for resistance biology of
agricultural
	crops: Cereal mildews, rusts and Septoria. Short presentation
10-20
	minutes including discussion.
=09
	12:00-13:00 Lunch

	13:00-17:30 COST817 on population studies of airborne pathogens
on
		 cereals 1993-98

		Welcome
		Main achievements in COST817
		New  COST action from January 1st 2000

	Specific achievements within population studies of airborne
pathogens
	on cereals and partial resistance to these. Invited speakers
will=20
	cover the first two general topics, and the following subjects
are =20
	presented by WG1 subgroup co-ordinators  and co-ordinators of
disease=20
	nurseries. There will be approx. 30 minutes for each of the
	following topics
=20
		Analysis of population survey data
		Analysis of partial resistance in cereals
		Wheat and barley brown rust
		Wheat yellow rust
		Wheat powdery mildew=20
		Septoria tritici =20
		Barley powdery mildew=20

		Conclusion by chair of session=09
=09
	19:00 - 	Dinner and social

Friday 27. November =20

	8:30-11:00 COST817 joint WG1 and WG4 meetings with subdivision =09
		according to disease system. WGI subgroup co-ordinators
		and WG4 disease nursery co-ordinators prepare agenda

		Wheat and barley brown rust
		Wheat yellow rust
		Wheat powdery mildew=20
		Septoria tritici =20
		Barley powdery mildew=20

	11:00-12:00 Joint WGI/WG4 meeting
		Conclusions/ activities in 1999
		The way forward in new action

	12:00	Lunch


	13:00-16:00 Excursion to Research Centre Flakkebjerg; Danish=20
		Institute of Agricultural Sciences, DK-4200 Slagelse,=20
		or Risoe National Laboratory.
	=09

Local organisers: Lisa Munk (NJF) and Mogens Hovmoller (COST) in=20
collaboration with Hanne =9Dstergaard and WG1 subgroup co-ordinators =
and=20
WG4 disease nursery co-ordinators


yours sincerely

Mogens Hovm=F8ller
Danish Institute of Agricultural Sciences
Research Centre Flakkebjerg
DK-4200 Slagelse
Denmark

phone:  +45 5811 3461 (direct)
fax:       +45 5811 3301
e-mail:  Mogens.Hovmoller@agrsci.dk




IMPORTANT:

Please indicate a preliminary interest to participate by filling in the
form=20
below, and reply to me not later than August 1st 1998 through this
E-mail=20
(or by fax). A more detailed programme and practical information will =
be
sent=20
out approximately Sepember 1st  to those who have shown interest. Final =

registration and payment must be received by October 15 1998.

=20
------------------------------------------------------------------------=

-----------

NAME:

ADDRESS:

FAX:

E-MAIL:

I would like to participate in the following activities (preliminary):

					Yes / No
November 25:
	COST817 MC-meeting

	NJF- afternoon
=09
November 26:
	NJF-morning

	COST817 afternoon

November 27:
	COST WG meetings morning

	Excursion to Flakkebjerg=20

	Excursion to Risoe

From BIOSCI-REQUEST  Fri Jun 12 06:56:54 1998
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Date: Fri, 12 Jun 1998 15:53:47 -0200
Subject: forma specialis
To: "'rust-mil'" <rust-mil@net.bio.net>
Errors-to: Rients.Niks@users.pv.wau.nl
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Dear colleagues,

In some texts  I have to use the plural of  FORMA  SPECIALIS.
I know that the plural of "curriculUM vitae" is "curriculA vitae" (second 
part, vitae is genitive of vita)

In the same way the plural should be: "formae specialis" ?
Or is specialis an adjective? Then the plural should be "formae speciales"?

How is the term conventionally abbreviated? "f.sp." becomes in plural 
"ff.sp."?

Who can help me in this?

Rients Niks
Department of Plant Breeding,
P.O. Box 386,
6700 AJ  Wageningen, 
the Netherlands

tel +31 317 482508
fax +31 317 483457
e-mail: rients.niks@users.pv.wau.nl



From BIOSCI-REQUEST  Sun Jun 14 02:35:52 1998
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Subject: Successful People Only
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Successful People Only Please:   Non-profit foundation seeking participants in 
creating great personal wealth and for self study course taught by 
multimillionaire in how to have what always is wanted.

Required: Prior success in some field, willingness to learn, single minded focus 
for six-month project, Desire for the freedom that personal wealth creates, and 
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Call toll free 888-303-0652 for a brief introduction 24 hrs.
 
 
 
 
 
 

From BIOSCI-REQUEST  Sun Jun 14 21:33:04 1998
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Subject: Earn $100 every time OUR phone rings...test
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Date: 14 Jun 1998 21:29:39 -0700

Testing...n


From BIOSCI-REQUEST  Mon Jun 15 02:25:50 1998
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Date: Mon, 15 Jun 1998 02:21:42 -0700
From: <cabletv@208.4.7.8>
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Subject: CABLE BOX DESCRAMBLERS
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To be removed from our mailing list simply reply with "REMOVE" in the subject.

Cable T.V. Descramblers, Converters, And Accessories for sale. We carry cable 
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this email and get a free gift with your perchase.($40 value). 
 
 
 
 
 
 
 
 
 
 
 

From BIOSCI-REQUEST  Mon Jun 15 05:59:08 1998
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From: "james.brown" <james.brown@bbsrc.ac.uk>
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To: rust-mil@net.bio.net
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X-Dmw-Body-Names: junk mail on rust-mil
Date: Mon, 15 Jun 98 13:56:20 +0100
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One member of the Rust-Mil mailing list has complained about junk mail
being sent to the Rust-Mil list. I've checked the archives, from which
I see that about three junk mails are being sent to Rust-Mil each
month, advertising various things.

The person who complained about junk mail asked me if I would consider
moderating the list (i.e. checking mails for relevance before they're
posted to the list). Do other members of Rust-Mil consider that the
amount of junk mail justifies someone moderating the list? The
down-side to this is that postings will inevitably be delayed,
especially when I'm on holiday.

James Brown
_________________________

Rust-Mil list coordinator

From BIOSCI-REQUEST  Mon Jun 15 12:26:26 1998
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From: "james.brown" <james.brown@bbsrc.ac.uk>
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To: rust-mil@net.bio.net
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I've had 8 replies to my message earlier today about junk mail, all of
which say that the Rust-Mil list should continue not to be moderated.

There's definitely a problem with people sending junk mail to BioNet
lists - they're not members of the list, so there's not a great deal
one can do about it. If you're a member of several BioNet lists,
you'll just have to get used to hitting the "delete" key!

James Brown

From BIOSCI-REQUEST  Wed Jun 17 00:17:35 1998
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Date: Tue, 16 Jun 1998 13:19:29 +0100
To: "james.brown" <james.brown@bbsrc.ac.uk>
Cc: rust-mil@net.bio.net
From: Roy Johnson <rjohnson@rjohnson.demon.co.uk>
Subject: Re: junk mail on rust-mil
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In message <980615135620.681@mserv.jic.bbsrc.ac.uk.0>, "james.brown"
<james.brown@bbsrc.ac.uk> writes
>One member of the Rust-Mil mailing list has complained about junk mail
>being sent to the Rust-Mil list. I've checked the archives, from which
>I see that about three junk mails are being sent to Rust-Mil each
>month, advertising various things.
>
>The person who complained about junk mail asked me if I would consider
>moderating the list (i.e. checking mails for relevance before they're
>posted to the list). Do other members of Rust-Mil consider that the
>amount of junk mail justifies someone moderating the list? The
>down-side to this is that postings will inevitably be delayed,
>especially when I'm on holiday.
>
>James Brown
>_________________________
>
>Rust-Mil list coordinator
Dear James, you should not spend any of your valuable time in moderating
the rust-mil mail. It is perfectly easy to hit the delete button, and
three times is a month is much less than I do that to messages.

Best wishes from Roy
-- 
Roy Johnson
Phone/Fax +44 1223 842507


From BIOSCI-REQUEST  Wed Jun 17 12:49:03 1998
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Date: Wed, 17 Jun 1998 14:52:31 -0600
To: rust-mil@net.bio.net, grains@greengenes.cit.cornell.edu,
        ARABIDOPSIS/bionet.genome.arabidopsis@iastate.edu,
        MAIZE/bionet.maize@iastate.edu
From: "Roger P. Wise" <rpwise@iastate.edu>
Subject: Postdoctoral Position - Molecular Genetics of Host/Pathogen
 Interaction

<bold><fontfamily><param>Times</param><bigger><bigger><bigger>Postdoctoral
Research Associate

Genetics / Molecular Biology of Host Resistance=20

to Fungal Pathogens


</bigger></bigger></bigger></fontfamily></bold><fontfamily><param>Times</par=
am><bigger><bigger>Postdoctoral
Research Associate (Plant Molecular Geneticist) available to
investigate the genetics and molecular biology of resistance to the
obligate fungal pathogen, <italic>Erysiphe graminis</italic>, in
barley.  The candidate will be responsible for molecular, genetic, and
functional characterization of gene-specific sequences identified on a
200-kb BAC contig spanning the <italic>Mla</italic> resistance-gene
cluster.  Approaches may include, but are not limited to, isolation of
cDNAs specific for candidate alleles, characterization of new
<italic>Mla</italic>-mutant specificities, functional analyses of
candidate alleles via barley and wheat transformation, or use of
expressed resistance gene sequences as traps in the yeast two-hybrid
system to identify interacting gene products in the host and/or
pathogen.


These approaches will be facilitated by our well-characterized,
high-resolution, recombinant mapping populations, and will complement
ongoing projects on the genetics of resistance to obligate fungal
biotrophs in cereal crops.  Ph.D. in genetics, molecular biology, plant
pathology or related field.  Experience in manipulation and cloning of
large DNA fragments, gene mapping, computational  analyses, and/or
cereal transformation is desirable.  Initial appointment is for two
years; salary starts at $31,897 per year + benefits.  Some limitations
on citizenship may apply.  Highly-motivated individuals should send
curriculum vitae, reprints, and three references (name, e-mail, phone,
address, and fax) to:


Dr. Roger Wise, USDA-ARS

Department of Plant Pathology=20

351 Bessey Hall

Iowa State University

Ames, IA, 50011-1020

Telephone:  (515) 294-9756 =20

=46ax: 	   (515) 294-9420

E-mail:	   rpwise@iastate.edu



<bold><underline>About Iowa State University


</underline></bold>Ames is a small city of 50,000 people, about half of
whom are students.  Affordable housing is available as well as a
first-rate public transportation system.  It is also easy to get around
town by bicycle.  The city and the University have numerous parks and
recreation facilities, and the Iowa State Center, home of Hilton
Coliseum, C.Y. Stephens Auditorium and Fisher Theater, hosts numerous
music concerts, live performances, and athletic events throughout the
year. =20


<bold><underline>About the Wise Lab

</underline>

</bold>The PI's laboratory contains ca. 1000 square feet of space and
has been approved by the Iowa State University Biohazards Safety
Committee for work with recombinant DNA at a BL2 containment level.=20
The lab is on the 4th floor (shared with ten other plant-molecular
biology laboratories) of Bessey Hall on the ISU campus.  Sufficient
cold storage facilities are available for long-term storage of seed
stocks in the Seed Science Center across the street.  We also maintain
a 1000 square foot room equipped with 400-watt sodium lamps in the
ISU-Plant Pathology greenhouse and 3 - 4 growth chambers.=20


All equipment required for recombinant DNA research is contained within
the PI's laboratory.  These items include balances, refrigerators, -20
and -80 freezers, refrigerated high-speed and microcentrifuges,
incubators, incubator shakers; two hybridization ovens, numerous
autoradiography cassettes and intensifying screens; horizontal,
vertical, and two CHEF Mapper pulse-field gel electrophoresis
apparatus; power supplies, water baths, pH meter, and three MJ Research
programmable thermal cyclers.  Items available as communal equipment
located in a central-user facility on the same floor include
ultracentrifuges, visible-UV spectrofluorimeter, electroporator, liquid
nitrogen supply, gel dryers, incubator shakers, UV transilluminators
and gel imaging system, autoclaves, speed-vac, water deionizers,
scintillation counter, spectrophotometers, laminar flow hoods, a
PDS-1000 Biolistic Gun (DuPont/Bio-Rad) and a regulated growth room for
tissue culture.  An Internet-connected Power Macintosh 8500/200 is
contained within the PI's office, and several other Macintosh computers
are available within the PI's program. Mapmaker version 3.0 is
available through ISU's UNIX mainframe, accessible from a work station
in the Plant Pathology Computer Facility on the same floor as the PI's
laboratory.  MapManager v2.6.5 software for Macintosh is currently
licensed by the PI.  The UWGCG DNA sequence analysis software on the
University's Vax cluster is accessible directly from the PI's office or
through the Plant Pathology Computer Facility.=20


<bold><underline>Other Resources</underline>


</bold>Iowa State University has several centers on campus capable of
assisting in a variety of techniques as well as supplying a wide range
of specialized equipment and expertise.=20


The newly established ISU <bold>Plant Transformation Facility</bold>
(PTF) provides maize and soybean transformation services to ISU
researchers (advice is available for other crops as well). Dr. Kan
Wang, the director of the PTF, has extensive industrial experience in
the transformation of maize.  The PTF is staffed by two M.S.-level
transformation specialists.


The <bold>Nucleic Acids Facility</bold> operates a Biosearch 8750
four-column DNA synthesizer for oligonucleotide synthesis, several
Applied Biosystems Automated DNA sequencers, three Waters HPLC systems
for automated purification of DNA, Perkin Elmer/Cetus thermal cycler,
and numerous computers, digitizers, and plotters.  These are operated
by two full-time technicians.  ISU will soon have a state-of-the art
<bold>DNA microarray facility</bold>.


The ISU <bold>Bessey Microscope Facility</bold> provides a variety of
instrumentation and technical assistance in light, scanning and
transmission electron microscopy and image analysis.  Equipment
includes a JEOL 1200/EX scanning/transmission electron microscope
(STEM), a Hitachi HS-8-2 transmission electron microscope (TEM) and a
JEOL JSM-35 scanning electron microscope. =20




</bigger></bigger></fontfamily>
_____________________________

Roger Wise, USDA-ARS

Department of Plant Pathology

409 Bessey Hall

Iowa State University

Ames, IA =20

50011-1020  USA

Phone:  515-294-9756

=46ax:    515-294-9420

E-mail: rpwise@iastate.edu

_____________________________


From BIOSCI-REQUEST  Wed Jun 17 13:43:08 1998
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Date: Wed, 17 Jun 1998 15:49:26 -0600
To: rust-mil@net.bio.net
From: "Roger P. Wise" <rpwise@iastate.edu>
Subject: Postdoc - Mol. Genetics of Host/Pathogen Interaction

<fontfamily><param>Times</param><bigger><bigger>Postdoctoral Research
Associate

Genetics / Molecular Biology of Host Resistance=20

to Fungal Pathogens


Postdoctoral Research Associate (Plant Molecular Geneticist) available
to investigate the genetics and molecular biology of resistance to the
obligate fungal pathogen, Erysiphe graminis, in barley.  The candidate
will be responsible for molecular, genetic, and functional
characterization of gene-specific sequences identified on a 200-kb BAC
contig spanning the Mla resistance-gene cluster.  Approaches may
include, but are not limited to, isolation of cDNAs specific for
candidate alleles, characterization of new Mla-mutant specificities,
functional analyses of candidate alleles via barley and wheat
transformation, or use of expressed resistance gene sequences as traps
in the yeast two-hybrid system to identify interacting gene products in
the host and/or pathogen.


These approaches will be facilitated by our well-characterized,
high-resolution, recombinant mapping populations, and will complement
ongoing projects on the genetics of resistance to obligate fungal
biotrophs in cereal crops.  Ph.D. in genetics, molecular biology, plant
pathology or related field.  Experience in manipulation and cloning of
large DNA fragments, gene mapping, computational  analyses, and/or
cereal transformation is desirable.  Initial appointment is for two
years; salary starts at $31,897 per year + benefits.  Some limitations
on citizenship may apply.  Highly-motivated individuals should send
curriculum vitae, reprints, and three references (name, e-mail, phone,
address, and fax) to:


Dr. Roger Wise, USDA-ARS

Department of Plant Pathology=20

351 Bessey Hall

Iowa State University

Ames, IA, 50011-1020

Telephone:  (515) 294-9756 =20

=46ax: 	   (515) 294-9420

E-mail:	   rpwise@iastate.edu



<underline>About Iowa State University


</underline>Ames is a small city of 50,000 people, about half of whom
are students.  Affordable housing is available as well as a first-rate
public transportation system.  It is also easy to get around town by
bicycle.  The city and the University have numerous parks and
recreation facilities, and the Iowa State Center, home of Hilton
Coliseum, C.Y. Stephens Auditorium and Fisher Theater, hosts numerous
music concerts, live performances, and athletic events throughout the
year. =20


<underline>About the Wise Lab

</underline>

The PI's laboratory contains ca. 1000 square feet of space and has been
approved by the Iowa State University Biohazards Safety Committee for
work with recombinant DNA at a BL2 containment level.  The lab is on
the 4th floor (shared with ten other plant-molecular biology
laboratories) of Bessey Hall on the ISU campus.  Sufficient cold
storage facilities are available for long-term storage of seed stocks
in the Seed Science Center across the street.  We also maintain a 1000
square foot room equipped with 400-watt sodium lamps in the ISU-Plant
Pathology greenhouse and 3 - 4 growth chambers.=20


All equipment required for recombinant DNA research is contained within
the PI's laboratory.  These items include balances, refrigerators, -20
and -80 freezers, refrigerated high-speed and microcentrifuges,
incubators, incubator shakers; two hybridization ovens, numerous
autoradiography cassettes and intensifying screens; horizontal,
vertical, and two CHEF Mapper pulse-field gel electrophoresis
apparatus; power supplies, water baths, pH meter, and three MJ Research
programmable thermal cyclers.  Items available as communal equipment
located in a central-user facility on the same floor include
ultracentrifuges, visible-UV spectrofluorimeter, electroporator, liquid
nitrogen supply, gel dryers, incubator shakers, UV transilluminators
and gel imaging system, autoclaves, speed-vac, water deionizers,
scintillation counter, spectrophotometers, laminar flow hoods, a
PDS-1000 Biolistic Gun (DuPont/Bio-Rad) and a regulated growth room for
tissue culture.  An Internet-connected Power Macintosh 8500/200 is
contained within the PI's office, and several other Macintosh computers
are available within the PI's program. Mapmaker version 3.0 is
available through ISU's UNIX mainframe, accessible from a work station
in the Plant Pathology Computer Facility on the same floor as the PI's
laboratory.  MapManager v2.6.5 software for Macintosh is currently
licensed by the PI.  The UWGCG DNA sequence analysis software on the
University's Vax cluster is accessible directly from the PI's office or
through the Plant Pathology Computer Facility.=20


<underline>Other Resources</underline>


Iowa State University has several centers on campus capable of
assisting in a variety of techniques as well as supplying a wide range
of specialized equipment and expertise.=20


The newly established ISU Plant Transformation Facility (PTF) provides
maize and soybean transformation services to ISU researchers (advice is
available for other crops as well). Dr. Kan Wang, the director of the
PTF, has extensive industrial experience in the transformation of
maize.  The PTF is staffed by two M.S.-level transformation
specialists.


The Nucleic Acids Facility operates a Biosearch 8750 four-column DNA
synthesizer for oligonucleotide synthesis, several Applied Biosystems
Automated DNA sequencers, three Waters HPLC systems for automated
purification of DNA, Perkin Elmer/Cetus thermal cycler, and numerous
computers, digitizers, and plotters.  These are operated by two
full-time technicians.  ISU will soon have a state-of-the art DNA
microarray facility.


The ISU Bessey Microscope Facility provides a variety of
instrumentation and technical assistance in light, scanning and
transmission electron microscopy and image analysis.  Equipment
includes a JEOL 1200/EX scanning/transmission electron microscope
(STEM), a Hitachi HS-8-2 transmission electron microscope (TEM) and a
JEOL JSM-35 scanning electron microscope. =20






</bigger></bigger></fontfamily>
_____________________________

Roger Wise, USDA-ARS

Department of Plant Pathology

409 Bessey Hall

Iowa State University

Ames, IA =20

50011-1020  USA

Phone:  515-294-9756

=46ax:    515-294-9420

E-mail: rpwise@iastate.edu

_____________________________


From BIOSCI-REQUEST  Thu Jun 18 06:26:18 1998
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Date: Thu, 18 Jun 1998 16:25:01 +0300
To: rust-mil@net.bio.net
From: "Bob O'Hara" <bob.ohara@Helsinki.FI>
Subject: Mla8 in Europe
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Just a little matter that has been bothering me - part of the E. graminis
'folklore' is that Va8 (the virulence allele towards Mla8) is fixed in the
European population.  I have two questions:  What is the evidence for this,
and when was the last time anyone checked?  

In case this sounds obscure, I should point out that almost every survey
sample in Europe is taken using either Golden Promise or Pallas.  Both, of
course, have Mla8.

I will send a summary of any comments I receive to the list.

(BTW, please note that I've now moved to Helsinki!)


Bob

Bob O'Hara

University of Helsinki
Department of Ecology and Systematics
Division of Population Biology
PO Box 17 (Arkadiankatu 7)
FIN-00014 
University of Helsinki
Finland

email: bob.ohara@helsinki.fi
ICQ: 7178841
tel: +358-9-191 7382
fax: +358-9-191 7492

To find out more about our reasearch group, take a look at
http://www.helsinki.fi/science/metapop/
Or, before someone realises I'm not in Denmark any more, look at:
http://www.risoe.dk/pbk/pls/staff/robo.htm



From BIOSCI-REQUEST  Thu Jun 18 10:26:57 1998
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Date: 18 Jun 1998 10:22:33 -0700



     Just a quick note to inform you that the internet stock mutual fund has been showing incredible growth as of recent.  You may want to consider getting some stock now while its relatively low.

     This is a mutual fund of strictly internet-related companies that have the most potential to grow, given the exploding growth of this new business medium.

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     If you think I can be any help to you, feel free to call me.

Thank you,

Joe Campanella                     (440) 684-4368


From BIOSCI-REQUEST  Mon Jun 22 03:34:31 1998
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Date: Mon, 22 Jun 1998 12:36:06 -0700
To: "Bob O'Hara" <bob.ohara@Helsinki.FI>
From: Eckhard Limpert <eckhard.limpert@ipw.agrl.ethz.ch>
Subject: Re: Mla8 in Europe
Cc: rust-mil@net.bio.net

Dear Bob,

Your comment on fixation of a gene is interesting indeed. Well, although
many investigators used the cultivars you mention, someone might argue that
there are a lot of others without known r-gene. - However, there may be
other, more basic points to the story and to the use of the established
wording.=20

One of them relates to 'fixation' of an allele which we know means that no
other allele(s) for this locus exists in the population. The term is used
quite frequently in our field of research, but who can say anything on fixed
alleles for virulence or avirulence for populations of e.g. powdery mildews
and rusts? It is not even the tip of an iceberg that we are able to
investigate with these populations !

The established wording comes from the analysis of other populations that
are often by ten orders of magnitude or more smaller than the populations
considered in our business. Keeping this in mind, we should change our
wording. We could say, if this is the case, that the frequency of an allele
is close to 100%.  At a recent Conference of COST 817 in Prague we discussed
this point in Working Group 1, a comment on which can be found in the
minutes. I would not be surprised if there were differences in the frequency
of Va8 across Europe, which would be indeed interesting to know.=20

It may sound  too strict, but I believe it is correct to say that no
fixation of any allele for virulence or avirulence can be expected in
populations such as the cereal mildews due to both, population size and the
extent of gene flow among vast areas. This was pointed out in more detail in
a recent paper with Pavel Barto=9A (Limpert, E., & Barto=9A, P., 1997.=
 Analysis
of pathogen virulence as decision support for breeding and cultivar choice.
In: Resistance of crop plants against fungi, ed. H. Hartleb, H. Heitefuss
and H-H. Hoppe, Fischer, Jena, 401-424).

Best wishes to you, Bob, in Helsinki!  I am looking forward to further
comments on this matter.

Eckhard

____________________________________________

At 16,25 18.06.98 +0300, you wrote:
>Just a little matter that has been bothering me - part of the E. graminis
>'folklore' is that Va8 (the virulence allele towards Mla8) is fixed in the
>European population.  I have two questions:  What is the evidence for this,
>and when was the last time anyone checked? =20
>
>In case this sounds obscure, I should point out that almost every survey
>sample in Europe is taken using either Golden Promise or Pallas.  Both, of
>course, have Mla8.
>
>I will send a summary of any comments I receive to the list.
>
>(BTW, please note that I've now moved to Helsinki!)
>
>
>Bob
>
>Bob O'Hara
>
>University of Helsinki
>Department of Ecology and Systematics
>Division of Population Biology
>PO Box 17 (Arkadiankatu 7)
>FIN-00014=20
>University of Helsinki
>Finland
>
>email: bob.ohara@helsinki.fi
>ICQ: 7178841
>tel: +358-9-191 7382
>fax: +358-9-191 7492
>
>To find out more about our reasearch group, take a look at
>http://www.helsinki.fi/science/metapop/
>Or, before someone realises I'm not in Denmark any more, look at:
>http://www.risoe.dk/pbk/pls/staff/robo.htm




*************************************************************
=20
       Eckhard LIMPERT

       Phytopathology
       Institute of Plant Sciences
       Swiss Institute of Technology, ETH
       Universitaetsstr 2
       CH-8092 Zurich
       Switzerland=20

       phone  +41-1-632 33 87=20
       fax       +41-1-632 10 92
       email   Eckhard.Limpert@IPW.AGRL.ETHZ.CH

**************************************************************




From BIOSCI-REQUEST  Tue Jun 23 06:12:00 1998
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Date: Tue, 23 Jun 1998 14:57:51 -0200
Subject: formae speciales
To: rust-mil@net.bio.net
Errors-to: Rients.Niks@users.pv.wau.nl
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Dear colleagues,
 Some time ago, I asked your help on the use of the plural of "forma 
specialis". 

I got reply from 7 colleagues, and all agreed that the plural should be 
"formae speciales", since "specialis" is the female/male form of the adjective 
"specialis".

About the abbreviation there is less agreement: 

I was informed that the CBE Manual of style (6th edition) says: ff.sp

but the Dictionary of Fungi (8th edition 1995) says: ff.spp.

Since spp. is also used to indicate the plural of species (noun), it seems 
maybe more logical to use ff.sp ?

Thanks for all the reaction to my question.


Rients Niks
Department of Plant Breeding,
P.O. Box 386,
6700 AJ  Wageningen, 
the Netherlands

tel +31 317 482508
fax +31 317 483457
e-mail: rients.niks@users.pv.wau.nl





From BIOSCI-REQUEST  Fri Jun 26 03:43:10 1998
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Date: Fri, 26 Jun 1998 13:41:43 +0300
To: rust-mil@net.bio.net
From: "Bob O'Hara" <bob.ohara@Helsinki.FI>
Subject: Mla8 in Europe, replies
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Last week I asked what was the evidence that Va8 was fixed in European
mildew, and when was the last time anyone checked.  I received two replies
to the questions.  Christoph L=F6wer (in Giessen, Germany) told me that he
has found less than 1% avirulence towards Mla8 in his Turkish samples, but
he thinks the avirulence he does see could be due to contamination (and
presumably all the other naughty things mildew gets up to in virulence
tests).  Mogens Hovm=F6ller (Flakkerbjerg, Denmark) has tested several
thousand isolates from Igri (Mlra) over the last few years, and hasn't
found any that are avirulent on Pallas.  This just leaves the rest of
Europe...

Eckhard Limpert (Z=FCrich, Switzerland) made some comments about what we mea=
n
by 'fixation'.  Essentially, his point seems to be that it is extremely
unlikely that a virulence allele will be fixed (all the members of the
population have the virulence allele), and anyway it is impossible to test
the whole population. =20
This reminds me of the definition of a polymorphism.  The definition I was
taught as an undergraduate was that a gene is polymorphic if different
alleles exist in that population at a frequency significantly greater than
the mutation rate.  Presumably an allele should therefore be considered
fixed if the gene isn't polymorphic.

Thanks to those that did reply, and I hope the weather wherever you are is
as nice as it is in Helsinki at the moment.


Bob

Bob O'Hara

University of Helsinki
Department of Ecology and Systematics
Division of Population Biology
PO Box 17 (Arkadiankatu 7)
FIN-00014=20
University of Helsinki
Finland

email: bob.ohara@helsinki.fi
ICQ: 7178841
tel: +358-9-191 7382
fax: +358-9-191 7492

To find out more about our reasearch group, take a look at
http://www.helsinki.fi/science/metapop/
Or, before someone realises I'm not in Denmark any more, look at:
http://www.risoe.dk/pbk/pls/staff/robo.htm



