From owner-vectors@net.bio.net Tue Jul 08 23:00:00 1997 Path: biosci!biosci!not-for-mail From: STEFAN.KUEPPER@DENOTES.henkel.de Newsgroups: bionet.biology.vectors Subject: stinging apparatus of mosquitoes? //97.07.08 18:09:28 Date: 9 Jul 1997 04:41:30 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 38 Sender: daemon@net.bio.net Approved: vbmod@klab.agsci.colostate.edu Distribution: world Message-ID: <5pvtda$7q0@net.bio.net> NNTP-Posting-Host: net.bio.net Dr. Stefan Kuepper Dusseldorf (Germany) 1997/07/08 Dear reader, I'm a german chemist and thus in no way firmly grounded in biologists tech-talk, so please do excuse inappropiate descriptions of insect body parts. My question: I would like to know whether someone could kindly provide me with any information regarding the stinging apparatus of mosquitoes or similiar insects (wasps, etc. not bees, because they do not retrieve their sting, you get the idea...). So, if if anyone out there knows something about scientists, research institutes or groups which look intensely into the stinging apparatus (meaning the "needle") of mosquitos and similiar "bugs".(SEM, TEM-pictures, mechanisms of skin-penetration, chemical composition etc., or maybe just an comprehensive literature review regarding either topic) please do not hesitate to contact me use any of the adresses given below: phone: + 49 211 797 7390 Fax: + 49 211 798 10050 e-mail: stefan.kuepper@denotes.henkel.de Any help is highly appreciated. Have a nice day. With kind regards , sincerely Stefan Kupper From owner-vectors@net.bio.net Sun Jul 13 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Eric Rappaport Newsgroups: bionet.biology.vectors Subject: DNA SEQUENCERS FOR SALE Date: 14 Jul 1997 12:14:14 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 65 Sender: daemon@net.bio.net Approved: vbmod@klab.agsci.colostate.edu Distribution: world Message-ID: <5qdtq6$o3m@net.bio.net> NNTP-Posting-Host: net.bio.net AUTOMATED DNA SEQUENCERS FOR SALE Three ABI 373 DNA sequencers, all currently in use and under service contract are available for sale. Please contact Dr. Eric Rappaport (rappaport@email.chop.edu) with offers or questions. Details of the equipment configuration follow: Two 373 units with Stretch upgrades: Filters - 5-filter wheel (can choose between 2 different sets of 4 different dyes). Chemistries - Standard Taq (FS) terminator, dye primer or ABI T7 polymerase sequencing chemistry. All current Genescan chemistries, including microsatellite panels, primers labeled during synthesis with standard ABI dyes (6-Fam, Hex, Tet, Tamra) or (6-Fam, Hex, Tamra, and Rox). Gel lengths - Multiple gel (well-to-read) lengths including 48 cm (standard long-read [>650 nt] sequencing length), 34 (shorter sequence reads [550-600 nt] or high-resolution Genescan applications), 24 (microsatellite applications) 12 (lower-resolution Genescan applications) or 6 (lower-resolution Genescan applications). Scan Speed - 1X, BaseSprinter (2X, but limitedto middle half of gel; can be used for faster analysis of shorter sequences). One 373 with multiple gel length (Leon) configuration: Filters - 5-filter wheel (can choose between 2 different sets of 4 different dyes). Chemistries - Standard Taq (FS) terminator, dye primer or ABI T7 polymerase sequencing chemistry. All current Genescan chemistries, including microsatellite panels, primers labeled during synthesis with standard ABI dyes (6-Fam, Hex, Tet, Tamra) or (6-Fam, Hex, Tamra, and Rox). Gel lengths - Multiple gel (well-to-read) lengths including 24 cm (shorter sequence reads [400-500 nt] or microsatellite applications) 12 (lower-resolution Genescan applications) or 6 (lower-resolution Genescan applications). Scan Speed - 1X, BaseSprinter (2X, but limitedto middle half of gel; can be used for faster analysis of shorter sequences). Computers and Software Unit 1 (Stretch) - Mac IIcx with 8 mB RAM/230 mB hard drive Unit 2 (Stretch) - Mac IIci with 8 mB RAM/100 mB hard drive Unit 3 (Leon) - Mac IIci with 8 mB RAM/120 mB hard drive All units are equipped with latest versions of analysis and collection sofware for running sequencing and Genescan applications on the above computers. Upgrades for PowerMac are not included. Service Contracts All units have been under service contract since the time of their purchase from ABI and are currently covered under those agreements. Purchaser would need to have an installation service call from ABI before initiation of a new service contract could occur. From owner-vectors@net.bio.net Sun Jul 27 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Michal Opas Newsgroups: bionet.biology.vectors Subject: Calreticulin Meeting: 1st circular Date: 28 Jul 1997 09:20:12 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 325 Sender: daemon@net.bio.net Approved: vbmod@klab.agsci.colostate.edu Distribution: world Message-ID: <5rigrs$kg2@net.bio.net> NNTP-Posting-Host: net.bio.net Dear Colleague, We are delighted to announce that Calreticulin Workshop,=20 devoted to the structure and function of calreticulin and=20 related proteins, will take place on March 31 - April 2,=20 1998 in Banff, Alberta, Canada. The Workshop will provide=20 unique opportunity to meet and interact with the scientists=20 interested in calreticulin research in spectacular=20 surroundings of Banff National Park in Canadian Rocky=20 Mountains. We are sure that the Banff Calreticulin Workshop=20 will be an important forum to share the latest findings and=20 to develop future interactions. Calreticulin has been=20 implicated to play a role in almost every aspect of cell=20 biology as outlined in a brief overview below. We hope that=20 the Workshop will be useful to sort out some of the latest=20 discoveries and controversies concerning calreticulin and=20 implication of this protein in a variety of biological=20 systems. On the behalf of the Organizing Committee we would=20 like to invite you to participate in the Workshop.=20 The Calreticulin Workshop is a satellite meeting to the 8th=20 Fisher Winternational Symposium on Cellular and Molecular=20 Biology which will be held April 2-5, 1998, also at the=20 Banff Conference Centre. The Winternational Symposium,=20 which is co-sponsored by our Society and Fisher Scientific,=20 is held annually, with a different focus each year. The=20 theme for the 1998 meeting is : "Membrane Proteins in Health=20 and Disease." Further information about the Fisher=20 Winternational or the satellite meetings can be obtained by=20 visiting the Society's web site at=20 http://www.csbmcb.ca/english/bulletin/winternational.e.html=20 or by contacting the Chair of the Scientific Program=20 Committee by E-mail: Carol Cass . I hope you will participate in the Calreticulin Workshop. =20 If you would like to receive further information please send=20 a request (if possible, by e-mail) to Michal Opas at: =09 m.opas@utoronto.ca =20 or at: =09Department of Anatomy & Cell Biology =09University of Toronto =09Medical Sciences Building =09Toronto, Ontario, M5S 1A8 Canada =09 tel: (416) 978-8947 =09 fax: (416) 978-3954 I look forward to hearing from you in the near future. For The Organizing Committee Sincerely yours Michal Opas Calreticulin, a multifunctional protein Calreticulin, 60 kDa Ca-binding protein [1], is a major=20 component of the endoplasmic reticulum (ER) of non-muscle=20 cells [2-7]. The protein is of high physiological=20 importance as it knockout is embryonic lethal [8]. Along=20 with a wide tissue distribution [9], calreticulin is present=20 in diverse animal and plant species [10]. calreticulin is a=20 resident ER protein as demonstrated by a variety of=20 biochemical and immunological techniques [1,3,4,6,11]. The=20 protein is synthesized with an N-terminal signal sequence=20 and it terminates with the KDEL sequence [3,12] which is=20 responsible for retrieval of proteins to the lumen of the ER=20 [13,14]. =20 =09Calreticulin functions in vivo as a Ca storage=20 protein [15,16]. It also has been well established that=20 calreticulin is a chaperone [17-21] and it shows similarity=20 in amino acid sequence to a part of calnexin, an ER membrane=20 chaperone [22]. The Ca storage and chaperone functions of=20 calreticulin are consistent with both the ER localization of=20 calreticulin and its structure. Stable overexpression of=20 calreticulin increases both cell-substratum and cell-cell=20 adhesiveness with concomitant upregulation of=20 adhesion-specific cytoskeletal protein, vinculin [23]. =20 Upregulation of calreticulin also affects adhesion-dependent=20 phenomena such as cell motility (which decreases) and cell=20 spreading (which increases). Downregulation of calreticulin=20 brings about inverse effects. In addition to the Ca=20 storage and chaperone function, calreticulin modulates gene=20 expression [24,25]. In vitro, calreticulin interaction with=20 the DNA binding domain of the glucocorticoid receptor=20 prevents the receptor from interacting with its=20 glucocorticoid response element [24]. Transcriptional=20 activation by glucocorticoid and androgen receptors in vivo=20 is inhibited in cells overexpressing full length=20 calreticulin [24,25]. Calreticulin itself is=20 stress-regulated by heat and heavy metals [26-28]. =20 Calreticulin has antithrombotic activity [29]. A host of=20 other putative calreticulin functions includes a role in=20 autoimmune diseases [30-34]. The protein affects=20 replication of the Rubella virus RNA [35,36]. In cytolytic=20 T lymphocytes it is found in the lytic granules where it may=20 play a role in killing of target cells [37]. In human=20 neutrophils calreticulin may contribute to the process of=20 phagocytosis [38]. In line with the reported functional=20 diversity, calreticulin was reported to be present in most=20 cellular compartments [10,11,37,39,40], including the outer=20 cell surface [41,42]. Recent hypotheses regarding=20 calreticulin function have been presented by Krause and=20 Michalak [43]. =09=09References 1. Ostwald TJ, MacLennan DH: Isolation of a high affinity=20 calcium binding protein from sarcoplasmic reticulum. J Biol=20 Chem 1974, 249:974-979. 2. Baksh S, Michalak M: Expression of calreticulin in=20 Escherichia coli and identification of its Ca2+ binding=20 domains. J Biol Chem 1991, 266:21458-21465. 3. Fliegel L, Burns K, Opas M, Michalak M: The=20 high-affinity calcium binding protein of sarcoplasmic=20 reticulum. Tissue distribution, and homology with=20 calregulin. Biochim Biophys Acta 1989, 982:1-8. 4. Opas M, Dziak E, Fliegel L, Michalak M: Regulation of=20 expression and intracellular distribution of calreticulin, a=20 major calcium binding protein of nonmuscle cells. J Cell=20 Physiol 1991, 149:160-171. 5. Milner RE, Baksh S, Shemanko C, Carpenter MR, Smillie L,=20 Vance JE, Opas M, Michalak M: Calreticulin, and not=20 calsequestrin, is the major calcium binding protein of=20 smooth muscle sarcoplasmic reticulum and liver endoplasmic=20 reticulum. J Biol Chem 1991, 266:7155-7165. 6. Michalak M, Baksh S, Opas M: Identification and=20 immunolocalization of calreticulin in pancreatic cells: no=20 evidence for "calciosomes". Exp Cell Res 1991, 197:91-99. 7. Michalak M, Milner RE, Burns K, Opas M: Calreticulin.=20 Biochem J 1992, 285:681-692. 8. Coppolino MG, Woodside MJ, Demaurex N, Grinstein S,=20 St-Arnaud R, Dedhar S: Calreticulin is essential for=20 integrin-mediated calcium signalling and cell adhesion.=20 Nature 1997, 386:843-847. 9. Tharin S, Dziak E, Michalak M, Opas M: Widespread tissue=20 distribution of rabbit calreticulin, a non-muscle functional=20 analogue of calsequestrin. Cell Tissue Res 1992, 269:29-37. 10. Opas M: The intracellular distribution and expression=20 of calreticulin. In Calreticulin, edited by Michalak M.=20 Georgetown: R.G. Landes; 1996:31-41. 11. Koch GLE: The endoplasmic reticulum and calcium=20 storage. BioEssays 1990, 12:527-531. 12. Fliegel L, Burns K, MacLennan DH, Reithmeier RAF,=20 Michalak M: Molecular cloning of the high affinity=20 calcium-binding protein (calreticulin) of skeletal muscle=20 sarcoplasmic reticulum. J Biol Chem 1989, 264:21522-21528. 13. Pelham HRB: Control of protein exit from the=20 endoplasmic reticulum. Annu Rev Cell Biol 1989, 5:1-23. 14. S=F6nnichsen B, F=FCllekrug J, Van PN, Diekmann W, Robinson=20 DG, Mieskes G: Retention and retrieval: Both mechanisms=20 cooperate to maintain calreticulin in the endoplasmic=20 reticulum. J Cell Sci 1994, 107:2705-2717. 15. Bastianutto C, Clementi E, Codazzi F, Podini P, De=20 Giorgi F, Rizzuto R, Meldolesi J, Pozzan T: Overexpression=20 of calreticulin increases the Ca2+ capacity of rapidly=20 exchanging Ca2+ stores and reveals aspects of their lumenal=20 microenvironment and function. J Cell Biol 1995,=20 130:847-855. 16. Liu N, Fine RE, Simons E, Johnson RJ: Decreasing=20 calreticulin expression lowers the Ca2+ response to=20 bradykinin and increases sensitivity to ionomycin in=20 NG-108-15 cells. J Biol Chem 1994, 269:28635-28639. 17. Nauseef WM, McCormick SJ, Clark RA: Calreticulin=20 functions as a molecular chaperone in the biosynthesis of=20 myeloperoxidase. J Biol Chem 1995, 270:4741-4747. 18. Wada I, Imai S, Kai M, Sakane F, Kanoh H: Chaperone=20 function of calreticulin when expressed in the endoplasmic=20 reticulum as the membrane-anchored and soluble forms. J Biol=20 Chem 1995, 270:20298-20304. 19. Nigam SK, Goldberg AL, Ho S, Rhode MF, Bush KT, Sherman=20 MY: A set of endoplasmic reticulum proteins possessing=20 properties of molecular chaperones includes Ca2+-binding=20 proteins and members of the thioredoxin superfamily. J Biol=20 Chem 1994, 269:1744-1749. 20. Otteken A, Moss B: Calreticulin interacts with newly=20 synthesized human immunodeficiency virus type 1 envelope=20 glycoprotein, suggesting a chaperone function similar to=20 that of calnexin. J Biol Chem 1996, 271:97-103. 21. Hebert DN, Foellmer B, Helenius A: Calnexin and=20 calreticulin promote folding, delay oligomerization and=20 suppress degradation of influenza hemagglutinin in=20 microsomes. EMBO J 1996, 15:2961-2968. 22. Bergeron JJM, Brenner MB, Thomas DY, Williams DB:=20 Calnexin: a membrane-bound chaperone of the endoplasmic=20 reticulum. Trends Biochem Sci 1994, 19:124-128. 23. Opas M, Szewczenko-Pawlikowski M, Jass GK, Mesaeli N,=20 Michalak M: Calreticulin modulates cell adhesiveness via=20 regulation of vinculin expression. J Cell Biol 1996,=20 135:1913-1923. 24. Burns K, Duggan B, Atkinson EA, Famulski KS, Nemer M,=20 Bleackley RC, Michalak M: Modulation of gene expression by=20 calreticulin binding to the glucocorticoid receptor. Nature=20 1994, 367:476-480. 25. Dedhar S, Rennie PS, Shago M, Leung-Hagesteijn C-Y,=20 Yang H, Filmus J, Hawley RG, Bruchovsky N, Cheng H, Matusik=20 RJ, Gigu=E8re V: Inhibition of nuclear hormone receptor=20 activity by calreticulin. Nature 1994, 367:480-483. 26. Nguyen TQ, Capra JD, Sontheimer RD: Calreticulin is=20 transcriptionally upregulated by heat shock, calcium and=20 heavy metals. Mol Immunol 1996, 33:379-386. 27. Dreher D, Vargas JR, Hochstrasser DF, Junod AF: Effects=20 of oxidative stress and Ca2+ agonists on molecular=20 chaperones in human umbilical vein endothelial cells.=20 Electrophoresis 1995, 16:1205-1214. 28. Conway EM, Liu L, Nowakowski B, Steiner-Mosonyi M,=20 Ribeiro SP, Michalak M: Heat shock-sensitive expression of=20 calreticulin. In vitro and in vivo up-regulation. J Biol=20 Chem 1995, 270:17011-17016. 29. Kuwabara K, Pinsky DJ, Schmidt AM, Benedict C, Brett J,=20 Ogawa S, Broekman MJ, Marcus AJ, Sciacca RR, Michalak M,=20 Wang F, Pan YC, Grunfeld S, Patton S, Malinski T, Stern DM,=20 Ryan J: Calreticulin, an antithrombotic agent which binds to=20 vitamin K-dependent coagulation factors, stimulates=20 endothelial nitric oxide production, and limits thrombosis=20 in canine coronary arteries. J Biol Chem 1995,=20 270:8179-8187. 30. Karska K, Tuckova L, Steiner L, Tlaskalova-Hogenova H,=20 Michalak M: Calreticulin--the potential autoantigen in=20 celiac disease. Biochem Biophys Res Commun 1995,=20 209:597-605. 31. Boehm J, Orth T, Van Nguyen P, S=F6ling H-D: Systemic=20 lupus erythematosus is associated with increased=20 auto-antibody titers against calreticulin and grp94, but=20 calreticulin is not the Ro/SS-A antigen. Eur J Clin Invest=20 1994, 24:248-257. 32. Zhu J, Newkirk MM: Viral induction of the human=20 autoantigen calreticulin. Clin Invest Med 1994, 17:196-205. 33. Ben-Chetrit E: The molecular basis of the SSA/Ro=20 antigens and the clinical significance of their=20 autoantibodies. Br J Rheumatol 1993, 32:396-402. 34. McCauliffe DP, Sontheimer RD: Molecular=20 characterization of the Ro/SS-A autoantigens. J Invest=20 Dermatol 1993, 100:73S-79S. 35. Atreya CD, Singh NK, Nakhasi HL: The rubella virus RNA=20 binding activity of human calreticulin is localized to the=20 N-terminal domain. J Virol 1995, 69:3848-3851. 36. Singh NK, Atreya CD, Nakhasi HL: Identification of=20 calreticulin as a rubella virus RNA binding protein. Proc=20 Natl Acad Sci USA 1994, 91:12770-12774. 37. Dupuis M, Schaerer E, Krause K-H, Tschopp J: The=20 calcium-binding protein calreticulin is a major constituent=20 of lytic granules in cytolytic T lymphocytes. J Exp Med=20 1993, 177:1-7. 38. Stendahl O, Krause K-H, Krischer J, Jerstrom P, Theler=20 JM, Clark RA, Carpentier JL, Lew DP: Redistribution of=20 intracellular Ca2+ stores during phagocytosis in human=20 neutrophils. Science 1994, 265:1439-1441. 39. Nakamura M, Moriya M, Baba T, Michikawa Y, Yamanobe T,=20 Arai K, Okinaga S, Kobayashi T: An endoplasmic reticulum=20 protein, calreticulin, is transported into the acrosome of=20 rat sperm. Exp Cell Res 1993, 205:101-110. 40. Dedhar S: Novel functions for calreticulin: =20 Interaction with integrins and modulation of gene=20 expression. Trends Biochem Sci 1994, 19:269-271. 41. White TK, Zhu Q, Tanzer ML: Cell surface calreticulin=20 is a putative mannoside lectin which triggers mouse melanoma=20 cell spreading. J Biol Chem 1995, 270:15926-15929. 42. Gray AJ, Park PW, Broekelmann TJ, Laurent GJ, Reeves=20 JT, Stenmark KR, Mecham RP: The mitogenic effects of the B =20 chain of fibrinogen are mediated through cell surface=20 calreticulin. J Biol Chem 1995, 270:26602-26606. 43. Krause K-H, Michalak M: Calreticulin. Cell 1997,=20 88:439-443. =20 =20 =20 =20 Dr. Michal Opas Department of Anatomy & Cell Biology University of Toronto 1 King's College Circle Medical Sciences Building Toronto, Ontario, M5S 1A8 Canada =20 phone: (416) 978-8947 fax: (416) 978-3954 e-mail: m.opas@utoronto.ca www homepage: http://www.utoronto.ca/anatomy/opas/start.htm=20 =20 From owner-vectors@net.bio.net Sun Jul 27 23:00:00 1997 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.biology.vectors Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 28 Jul 1997 09:19:26 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 234 Sender: daemon@net.bio.net Approved: vbmod@klab.agsci.colostate.edu Distribution: world Message-ID: <5rigqe$ket@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-vectors@net.bio.net Wed Jul 30 23:00:00 1997 Path: biosci!biosci!not-for-mail From: LOUIS@nefeli.imbb.forth.gr Newsgroups: bionet.biology.vectors Subject: postdoc position Date: 31 Jul 1997 08:18:33 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 34 Sender: daemon@net.bio.net Approved: vbmod@klab.agsci.colostate.edu Distribution: world Message-ID: <5rqac9$4th@net.bio.net> NNTP-Posting-Host: net.bio.net I am posting the following message for some friends in Rome. K. Louis ********** POSTDOCTORAL RESEARCH FELLOW AT THE UNIVERSITY OF ROME, INSTITUTE OF PARASITOLOGY A postdoctoral position for a scientist from one of the EU-member states (excluding Italy) is available in the laboratory of Prof. Mario Coluzzi (Istituto di Parassitologia, Universita' di Roma "La Sapienza") to isolate and characterize cDNAs coding for receptors and secreted molecules from the salivary glands of the malaria vector Anopheles gambiae. The project is part of a TMR research network on "Insect-parasite interactions: molecular aspects of infection and immunity in Diptera" (additional info can be found at the URL http://konops.imbb.forth.gr/TMR/) The network includes the following partner laboratories: Fotis Kafatos (EMBL, Heidelberg), Mario Coluzzi (Ist. Parassitologia, Rome), Andrea Crisanti (Imperial College, London), Jules Hoffmann (CNRS, Strasbourg), Kitsos Louis (IMBB, Heraklion) and Bob Sinden (Imperial College, London). Candidates should have a PhD or equivalent experience and a strong background in molecular biology. Experience in molecular entomology and/or biology of disease vectors would be an advantage. The position is available for 2 years starting October 1997. Applications (including a CV and two letters of recommendation to be sent directly) should be sent to: Dr. Bruno Arca' Istituto di Parassitologia - Universita' di Roma "La Sapienza" P.le Aldo Moro 5, 00185 Roma - Italy. Fax: +39-6-4991-4644 E-mail: b.Arca@Caspur.it