From owner-vectors@net.bio.net Fri Feb 06 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Bob Liparulo Newsgroups: bionet.biology.vectors Subject: storing viruses Date: 7 Feb 1998 05:58:37 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Approved: vbmod@klab.agsci.colostate.edu Distribution: world Message-ID: <6bhpad$81h@net.bio.net> NNTP-Posting-Host: net.bio.net I recently read a novel that contained this line: "The virus should be stored at a constant six degrees centigrade, the ideal temperature for keeping the virus static, neither dying nor growing." Does this make sense? Can viruses be made "static" in that sense? At that temerature? How ARE viruses stored for research? If you can help me understand this subject more, please e-mail me. Thank you. Bob liparulo@rmi.net <- please email me From owner-vectors@net.bio.net Sun Feb 08 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Stewart M. Gray Newsgroups: bionet.biology.vectors Subject: Post doctoral Position Available Date: 9 Feb 1998 12:17:19 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 51 Sender: daemon@net.bio.net Approved: vbmod@klab.agsci.colostate.edu Distribution: world Message-ID: <6bno8f$be1@net.bio.net> NNTP-Posting-Host: net.bio.net Re advertisement of a USDA, ARS Postdoctoral Research Associate Position in the Department of Plant Pathology, Cornell University, Ithaca, NY Funding for 1 year with possibility of an additional 2 years. Available immediately. Applicants must be a US citizen and have completed their Ph.D. within the previous 4 years. Research will focus on the genetic basis of Luteovirus transmission by aphids. These viruses circulate in the aphid without replicating. The circulative route of virus movement through the aphid is well described (J. General Virology 1997, 78:495-503; Phytopathology 1993,83:1293-1302) and the role of virus proteins in the transmission process is a continuing focus of the research program (Virology 1995, 206:954-962 & 1996, 219: 57-65). The overall goal of the research program is to understand both the virus and aphid components that regulate transmission; i.e. the movement of virus through the hindgut and salivary tissues of the aphid and the survival of the virus in aphid hemolymph. The postdoctoral position will specifically be involved with the identification of genes in the aphid that are involved in virus transmission. We are investigating differences in vectoring capabilities among clones of one aphid species, Schizaphis graminum. Physical barriers to transmission are being identified and the effects of hemolymph associated factors in the degradation of virus are being examined. The incumbent will be expected to conduct detailed genetic analyses of parental populations of vectors and nonvectors. In addition, they will assist in the development and evaluation of an aphid population that can be used to map genes of interest. Expertise in differential display and related technologies is a must. Extensive skills in molecular biology and virology is also required. A working knowledge of plant virology, virus transmission and entomology is desireable. For further information please contact Dr. Stewart Gray USDA, ARS Department of Plant Pathology Cornell University Ithaca, NY 14853 607-255-7844 Stewart M. Gray Research Plant Pathologist, USDA, ARS Assoc. Professor, Dept. Plant Pathology Cornell University 334 Plant Science Ithaca, NY 14853 607-255-7844 (Off.) 607-255-4596 (Lab) 607-255-2459 (Fax) From owner-vectors@net.bio.net Thu Feb 12 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Benes, Helen Newsgroups: bionet.biology.vectors Subject: Aedes atropalpus libraries Date: 13 Feb 1998 15:23:05 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 18 Sender: daemon@net.bio.net Approved: vbmod@klab.agsci.colostate.edu Distribution: world Message-ID: <6c2kkp$rik@net.bio.net> NNTP-Posting-Host: net.bio.net Does anyone know if there are cDNA and/or genomic librarires available for Aedes atropalpus? Thanks for any information. [If this is inappropriately addressed, please excuse the intrusion.] Helen Benes Department of Biochemistry/Molecular Biology University of Arkansas for Medical Sciences, Slot 516 4301 West Markham St. Little Rock, AR 72205 Tel.: (501) 686-5782 FAX: (501) 686-8169 E-mail: beneshelen@exchange.uams.edu From owner-vectors@net.bio.net Wed Feb 18 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Don Newsgroups: bionet.biology.vectors Subject: Looking for a good set of reference manuals....... Date: 19 Feb 1998 14:53:09 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 25 Sender: daemon@net.bio.net Approved: vbmod@klab.agsci.colostate.edu Distribution: world Message-ID: <6cid4l$l9n@net.bio.net> NNTP-Posting-Host: net.bio.net Hello, I hope to acquire some personal insight into a good set of reference manual relating the general laboratory practices of a virological research laboratory. Information contain therein would be most beneficial if it included detailed information about: Viral culturing, and maintenance. Molecular techniques related to viral work. Reagent preparation and methodologies. Historical vector construction. Comprehensive viral schematics and know biology. Viral target tissues. and what ever else might be available. If you know of a series of manuals or separate manuals that when used in conjunction complete a good base foundation of viral laboratory techniques, I would be greatly appreciative to have the information necessary for their purchase. Don Roberts ecoman1@airmail.net From owner-vectors@net.bio.net Mon Feb 23 22:00:00 1998 Path: biosci!biosci!not-for-mail From: Michal Opas Newsgroups: bionet.biology.vectors Subject: Calreticulin Workshop: Program & Gen Info Date: 24 Feb 1998 08:50:16 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 215 Sender: daemon@net.bio.net Approved: vbmod@klab.agsci.colostate.edu Distribution: world Message-ID: <6cuto8$pqu@net.bio.net> NNTP-Posting-Host: net.bio.net Dear Colleague, We are delighted to announce that Calreticulin Workshop, devoted to the structure and function of calreticulin and related proteins, will take place on March 31 - April 2, 1998 in Banff, Alberta, Canada. The Calreticulin Workshop is a satellite meeting to the 8th Fisher Winternational Symposium on Cellular and Molecular Biology which will be held April 2-5, 1998, also at the Banff Conference Centre. The Workshop will provide unique opportunity to meet and interact with the scientists interested in calreticulin research in spectacular surroundings of Banff National Park in Canadian Rocky Mountains. We are sure that the Banff Calreticulin Workshop will be an important forum to share the latest findings and to develop future interactions. Calreticulin has been implicated to play a role in almost every aspect of cell biology as outlined in a brief overview below. We hope that the Workshop will be useful to sort out some of the latest discoveries and controversies concerning calreticulin and implication of this protein in a variety of biological systems. On the behalf of the Organizing Committee we would like to invite you to participate in the Workshop. Program of the 3rd Calreticulin Workshop "Wine & cheese" reception: March 31, 1998 8:00-10:30 pm Day 1 April 1 9:00-9:20 M. Opas Foreword ER Signalling 9:20-10:00 K. Mikoshiba "Molecular Dynamics in Brain Development" Calcium Chair: N.S. Allen 10:00-10:40 T. Pozzan "Calreticulin as a Ca2+-buffer in the ER: evidence from overexpression studies" 10:40-11:20 KH. Krause "Ligand-gated and store-operated Ca2+ channels" 11:20-11:40 Coffee Break Calcium continued Chair: K. Mikoshiba 12:00-12:40 P. Camacho "Modulation of intracellular Ca2+ release and uptake by Calreticulin, Calnexin and Calmegin in Xenopus oocytes". 12:40-13:40 Lunch Break ER Chaperones Chair: R. Sontheimer 13:40-14:20 J. Bergeron "ER chaperones" 14:20-15:00 W. Nauseef "Roles of calreticulin and calnexin in ER 'quality control'" 15:00-15:40 D. Hebert "Calreticulin and calnexin: ER chaperone partners" 15:40-16:00 Coffee Break Disease Chair: K-H. Krause 16:00-16:40 R. Sontheimer Calreticulin Autoantibody Detection Employing a Ro Autoantigen-Binding Form of Recombinant Human Calreticulin" 16:40-17:20 P. Eggleton "Dual roles of calreticulin in autoimmunity and innate immunity" 17:20-18:00 B. Ritchie "Calreticulin in Blood Coagulation" 18:00-18:40 G. Needham "Tick Calreticulin" Day 2 April 2nd Gene Chair: T. Pozzan 09:00-09:40 R. Clark "Mechanisms of transcriptional regulation of calreticulin expression" 09:40-10:20 R. St.Arnaud "Specific developmental roles for calreticulin revealed by the knock-out mice" 10:20-11:00 D. Llewellyn "Stress-induced calreticulin expression" 11:00-11:20 Coffee Break Gene continued Chair: P. Eggleton 11:20-12:00 Nakhashi "Calreticulin and RNA processing" Plants 12:00-12:40 Allen/Wyatt/Tsou/Robertson "Calreticulin: a tool to alter cellular calcium in plant cells" 12:40-13:20 Navazio/Mariani "Calreticulin in plant cells" 13:20-14:20 Lunch Break Trafficking Chair: J. Bergeron 14:20-15:00 Johnson "Calreticulin in neuronal cells" Adhesion 15:00-15:40 Murphy-Ullrich "Calreticulin as a mediator of thrombospondin-stimulated focal adhesion disassembly" 15:40-16:00 Coffee Break Adhesion continued Chair: R. Clark 16:00-16:40 Dedhar "Perturbation of integrin mediated adhesion and Ca signalling in calreticulin-deficient cells. 16:40-17:20 Opas "Modulation of cellular adhesiveness by levels of expression of calreticulin" 17:20 Closing discussion >>> Conclusion M. Michalak SCIENTIFIC PROGRAM The scientific sessions for the Workshop will take place on Wednesday, April 1, 1998 from 9:00 AM - circa 7:00 PM. Slide projector, overhead projector, black-board and VHS VCR will be available for your presentations. Presentations: Each speaker will have ~40 minutes for presentation: please make presentation short enough to leave ~10 minutes for discussion. As you can see from the scientific program we are all working in extremely diverse areas of the biological sciences. Therefore, please make sure you devote at least 5 minutes of your talk to introduce the biological system you are studying. There is no need to send abstracts for the Workshop. Posters: Bring one or more posters if you wish! Posters shall be displayed throughout the Workshop, but we shall not formal poster sessions. In keeping with informal manner of the Workshop we expect posters to be on display throughout the duration of the Workshop to provide ample opportunity to discuss them at any time. REGISTRATION & ACCOMMODATION Schedule arrival date is on Tuesday, March 31, 1998. Upon arrival to the Banff Center please register for your accommodation at the Front Office in the foyer of the first floor of Donald Cameron Hall (Administration Building). Check-in time for your room is 2:30 PM on your day of arrival. Check-out time is 12:00 noon on your departure day. Your account may be paid in cash or by credit card (Visa, American Express, Master Card, EnRoute). RECEPTION There will be a reception ("wine & cheese") held for the Workshop participants on Tuesday, March 31, 1998 from 8:00 PM - 10:30 PM. Registration packets for the Workshop and name tags will be available during the reception. WINTERNATIONAL SYMPOSIUM The Calreticulin Workshop is a satellite meeting to the 8th Fisher Winternational Symposium on Cellular and Molecular Biology entitled "Membrane Proteins in Health and Disease" which will be held April 2-5, 1998, also at the Banff Conference Centre. Information about the meetings and registration forms can be obtained by contacting: Dr. Carol E. Cass, Chair Winternational Symposium Department of Oncology University of Alberta Cross Cancer Institute Edmonton, Alberta T6G 1Z2 (403) 432-8320 phone (403) 432-8425 fax email: sherron.becker@cancerboard.ab.ca GROUND TRANSPORTATION Bus service by Brewster Transportation is available directly from Calgary International Airport to Banff at 12:30 PM, 3:00 PM and 6:00 PM. The travel time is about 2 hours and the current one way fare is $28.00. Upon request buses will drop off passengers at the Banff Center. Brewster Transportation numbers are 403-762-6767 and 1-800-661-1152. If you have any further questions or special requirements please contact: Marek Michalak at (403) 492-2256 phone (403) 492-0886 fax E-mail: marek.michalak@ualberta.ca or Michal Opas at: (416) 978-8947 phone (416) 978-3954 fax E-mail: m.opas@utoronto.ca SEE YOU IN BANFF! Cheers Marek Michalak & Michal Opas From owner-vectors@net.bio.net Fri Feb 27 22:00:00 1998 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.biology.vectors Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 28 Feb 1998 10:29:13 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 234 Sender: daemon@net.bio.net Approved: vbmod@klab.agsci.colostate.edu Distribution: world Message-ID: <6d9l1p$n9i@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! 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The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms.