From owner-vectors@net.bio.net Wed Jul 07 13:17:00 1999 Path: biosci!biosci!not-for-mail From: Paul Jasper Newsgroups: bionet.biology.vectors Subject: New Blasticidin Selection Date: 7 Jul 1999 07:17:41 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 29 Sender: daemon@net.bio.net Approved: vbmod@klab.agsci.colostate.edu Distribution: world Message-ID: <7lvni5$f52@net.bio.net> NNTP-Posting-Host: net.bio.net hey all, Invitrogen is marketing a new selection for eukaryotic cells called "Blasticidin" which supposedly selects faster than G418/neomycin. They claim that you can get stables in as little as 4 days.....and it is 1/4 the price of G418.... Has anyone tried this new selection?? How is it? Does it work as well as they say it does ??? let me know what you think. paul -- -- ___ ____ ____ ____ ____________________ ____ ____ ____ _ \ \ / \ \ / \ \ / \ \ / Paul Jasper \ / \ \ / \ \ / \ \ / /\ \ /\ \ /\ \ /\ Micro/Immuno - Loyola U. /\ \ /\ \ /\ \ \__\/ \__\/ \__\/ \__pjasper@orion.luc.edu__\/ \__\/ \__\/ \__\/ From owner-vectors@net.bio.net Fri Jul 16 11:24:00 1999 Path: biosci!biosci!not-for-mail From: ricardo Newsgroups: bionet.biology.vectors Subject: RECIPES for entomologist Date: 16 Jul 1999 05:24:26 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 45 Sender: daemon@net.bio.net Approved: vbmod@klab.agsci.colostate.edu Distribution: world Message-ID: <7mn89q$eum@net.bio.net> NNTP-Posting-Host: net.bio.net Dear colleagues, please be so kind and do not be upset for crossposting. I have just start section RECIPES at www.coleoptera.org in directory at bottom of the section < for collectors> Please be so kind and send to me any recipes of for relaxing specimens, microscopy, mounting of speciemns etc., glue recipes, how to get mould off the specimen, anything waht you feel that could be handy or interesting for entomologists. Thank you very much for your response Keep care and be of good cheer. Regards (name) Vratislav Richard Eugene Maria John Baptiste (surname) of Bejsak (Bayshark)-Collorado-Mansfeld http://www.coleoptera.org Coleoptera - Australia, Tenebrionidae of World (incl. Lagriinae, Alleculinae) University of Sydney The Wentworth Bldg., Box 62 NSW 2006 AUSTRALIA phone : +61 414 540 465 e-mail: vratislav@bigfoot.com ricardo@ans.com.au (before Ricardo@compuserve.com and ricardo@login.cz ) Only after the last tree has been cut down, only after the last river has been poisoned, only after the last fish has been caught, only then will you find that money can not be eaten.' CREE INDIAN PROPHECY. Incoming mail is certified Virus Free. Checked by AVG anti-virus system (http://www.grisoft.com). Version: 6.0.37 / Virus Database: 30 - Release Date: 6.4.1999 From owner-vectors@net.bio.net Fri Jul 16 11:25:00 1999 Path: biosci!biosci!not-for-mail From: AMBID Newsgroups: bionet.biology.vectors Subject: MSc Applied Molecular Biology of Infectious Diseases Date: 16 Jul 1999 05:25:35 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 32 Sender: daemon@net.bio.net Approved: vbmod@klab.agsci.colostate.edu Distribution: world Message-ID: <7mn8bv$f4r@net.bio.net> NNTP-Posting-Host: net.bio.net MSc in the Applied Molecular Biology of Infectious Diseases London School of Hygiene and Tropical Medicine Applications are invited for entry to this course in September 1999. Minimal entry requirements are of a class 2:2 degree or equivalent training and experience. Partial scholarship support might be available for some applicants. The course provides a thorough understanding and practical experience of molecular biology for students who wish to use this approach in clinical diagnosis, epidemiological investigations, or laboratory research on infectious diseases. Background will be given for those who have limited previous theoretical and practical experience of molecular biology. There is an individually designed research project under the supervision of experienced post-doctoral scientists. A high proportion of students subsequently enter PhD programmes. For more information and an application form contact: The Deputy Registrar, London School of Hygiene & Tropical Medicine, Keppel Street, London WC1E 7HT. Telephone: +44 (0) 171 927 2239. Fax: +44 (0) 171 323 0638. Email: registry@lshtm.ac.uk Enquiries for course organiser: Email: m.miles@lshtm.ac.uk; Telephone: +44 (0) 171 927 2249/2639; Fax: +44 (0) 171 636 8739. M. A. Miles The School is an exempt charity dedicated to providing excellence in teaching, learning and research for the benefit of health worldwide, and is an equal opportunities employer From owner-vectors@net.bio.net Sat Jul 17 11:13:00 1999 Path: biosci!biosci!not-for-mail From: news@bt.net Newsgroups: bionet.biology.vectors Subject: An Apology and a Plea for help Date: 17 Jul 1999 05:13:35 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 44 Sender: daemon@net.bio.net Approved: vbmod@klab.agsci.colostate.edu Distribution: world Message-ID: <7mps1f$bt2@net.bio.net> NNTP-Posting-Host: net.bio.net From: "Ellen Southworth" Newsgroups: bionet.biology.vectors,bionet.microbiology,bionet.virology,bionet.women-in-bio Subject: An Apology and a Plea for help Lines: 29 X-Priority: 3 X-MSMail-Priority: Normal X-Newsreader: Microsoft Outlook Express 5.00.2314.1300 X-MimeOLE: Produced By Microsoft MimeOLE V5.00.2314.1300 Message-ID: Date: Fri, 16 Jul 1999 18:55:40 +0100 NNTP-Posting-Host: 195.99.43.47 X-Trace: newreader.ukcore.bt.net 932148460 195.99.43.47 (Fri, 16 Jul 1999 19:07:40 BST) NNTP-Posting-Date: Fri, 16 Jul 1999 19:07:40 BST Firstly, please accept my sincere apologies for the crossposting. Not having a clue about the subject, I really didn't know which group to post to. Next, a little about me. I'm an aspiring writer (as yet unpublished) and I have just begun my third novel. Set in the near future, it centres around a virus/bacterium that begins life as a biological weapon and which is engineered into something far more sinister in the search for a cure. The main facts I need to know involve the potential method of infection (vector?), what such a thing would look like under a microscope, possible symptoms and, probably most important of all, just exactly what happens in a laboratory that deals with deadly bugs? What safety measures would be used, how would they be stored/handled etc. I have attempted to read a number of books on the subject, all of which have left me feeling glazed and confused. Please, if anyone is willing to help me with this, I would be eternally grateful for your e-mails. If you would like to know what this virus nasty becomes, I am willing to mail you a few appropriate passages. Thanking you in anticipation. -- ellen.southworth@lineone.net Sleep is no substitute for coffee (Anon.) From owner-vectors@net.bio.net Wed Jul 28 10:42:00 1999 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.biology.vectors Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 28 Jul 1999 04:42:30 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 234 Sender: daemon@net.bio.net Approved: vbmod@klab.agsci.colostate.edu Distribution: world Message-ID: <7nmqb6$a5c@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms.