I am trying to separate 1-10 micron Nuclear Polyhedrosis Virus
from microsporidia which is only slightly bigger, ca. 15 microns.
I have tried ultra-centrifugation on a sucrose gradient without
I ran some stock NPV on 0.3% and 0.7% agarose gels for
two hours today. There appeared to be a little movement in the 0.3%
gel. But it could just be that the wells are bulging. I'll know
tomorrow when I continue the run.
Meanwhile, I want to mix up some new gels to run along side the
one's I already have. There was an allusion to electrophoresis on a
sucrose gradient in a 1970's text on plant viruses. I'm tempted to mix
some sugar into the TAE buffer I used to make the 0.3% agarose gel.
Perhaps this will make it viscous enough so that I can further reduce the
concentration of agar?
PS. I'm in Bangkok and don't have direct access to news groups. Please
send replied directly to me at
daniel at chulkn.chula.ac.th