Dear virologists
I'm a Dutch PhD student working on a virus disease of the common
mushroom (Agaricus bisporus). At the moment we're trying to develop
a RNA polymerase assay for the isolated virus particle. For this
purpose we want to disrupt the isometric virus particle without
denaturing the viral proteins. In other words we want to avoid the
use of detergents, high salts etc. Does anyone have any experience on
this subject?
Ted van der Lende
Department of Plant Biology
University of Groningen
Kerklaan 30
9751 NN Haren
The Netherlands
email: LENDETR at biol.rug.nl