Message forwarded from Franco Felici {felici at irbm.it}
>From: IRBMV1::FELICI 16-MAR-1994 12:48:39.09
>To: WALLACE
>CC:
>Subj: se puo' essere di interesse generale...
>> #59 14-MAR-1994 13:05:13.77 PEPLIBS
>From: IRBMV1::FELICI
>To: SMTP%"plyhmb at pln1.nott.ac.uk"
>CC: FELICI
>Subj: RE:fd phage expression
>Dear Mark,
>>from my point of view, it can not be predicted "how large a polypeptide can be
>expressed, fused to the N terminal of the gene III product, without
>significantly affecting its function".
>As you know, the N-terminus of pIII is important for phage infectivity, I
>think that the size of the N-terminal fusion is not the only thing that has
>to be considered, also its expression, folding, capability to be translocated,
>composition, stability in E. coli are very (if not more...) important.
>A "strange" peptide could be worse than a large protein for pIII function.
>>Best wishes for your work.
>> Franco Felici
